LOCUS       sequence-82276-e        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary retroviral pl. (puromycin). Cat(PacI/ClaI):Linker 
            (GGGGS)X2+IFP-F[2]. In the second MCS, the Plum fluorescent protein 
            is cloned in between PmeI/SalI.Confers ampicillin resistance to 
            DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: pLpC-Cat-L-IFP-F[1]+Plum (mammalian)
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgggcaacg ccgccgccgc caagaagggc agcgagcagg agagcgtgaa agagttccta
       61 gccaaagcca aggaagattt cctgaaaaaa tgggagaccc cttctcagaa tacagcccag
      121 ttggatcagt ttgatagaat caagaccctt ggcaccggct cctttgggcg agtgatgctg
      181 gtgaagcaca aggagagtgg gaaccactac gccatgaaga tcttagacaa gcagaaggtg
      241 gtgaagctaa agcagatcga gcacactctg aatgagaagc gcatcctgca ggccgtcaac
      301 ttcccgttcc tggtcaaact tgaattctcc ttcaaggaca actcaaacct gtacatggtc
      361 atggagtatg tagctggtgg cgagatgttc tcccacctac ggcggattgg aaggttcagc
      421 gagccccatg cccgtttcta cgcggcgcag atcgtcctga cctttgagta tctgcactcc
      481 ctggacctca tctaccggga cctgaagccc gagaatcttc tcatcgacca gcagggctat
      541 attcaggtga cagacttcgg ttttgccaag cgtgtgaaag gccgtacttg gaccttgtgt
      601 gggacccctg agtacttggc ccccgagatt atcctgagca aaggctacaa caaggctgtg
      661 gactggtggg ctctcggagt cctcatctac gagatggctg ctggttaccc acccttcttc
      721 gctgaccagc ctatccagat ctatgagaaa atcgtctctg ggaaggtgcg gttcccatcc
      781 cacttcagct ctgacttgaa ggacctgctg cggaaccttc tgcaggtgga tctcaccaag
      841 cgctttggga acctcaagaa cggggtcaat gacatcaaga accacaagtg gtttgccacg
      901 actgactgga ttgccatcta tcagagaaag gtggaagctc ccttcatacc aaagtttaaa
      961 ggccctgggg acacgagtaa ctttgacgac tatgaggagg aagagatccg ggtctccatc
     1021 aatgagaagt gtggcaagga gtttactgag ttt
//
