LOCUS       sequence-82277-e         144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker 
            (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein
            is cloned in between PmeI/SalI.Confers ampicillin resistance to 
            DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 144)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)
FEATURES             Location/Qualifiers
     source          1..144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..129
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
ORIGIN
        1 atgaacactg aagccgccag gcgttctcgt gcgagaaagt tgcaaagaat gaaacaactt
       61 gaagacaagg ttgaagaatt gctttcgaaa aattatcact tggaaaatga ggttgccaga
      121 ttaaagaaat tagttggcga acgc
//
