LOCUS       sequence-82280-e        7746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Right hand TALEN against Aedes aegypti KMO gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Aryan A, Anderson MA, Myles KM, Adelman ZN
  TITLE     TALEN-based gene disruption in the dengue vector Aedes aegypti.
  JOURNAL   PLoS One. 2013;8(3):e60082. doi: 10.1371/journal.pone.0060082. Epub 
            2013 Mar 21.
  PUBMED    23555893
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(3):e60082. doi"; pages: " 
            10.1371/journal.pone.0060082. Epub 2013 Mar 21"
COMMENT     Sequence Label: pSLfa PUb TAL-KMO-R
FEATURES             Location/Qualifiers
     source          1..7746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..29)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(263..282)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(942..1733,1734..1802))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1565..1584
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1803..1907)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1975..1993
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2031..2053)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2153..2172
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2189..2644)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2326..2347)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2538..2557
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2842..2864
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2856..2873
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2857..2873
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4377..4397
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4404..4448
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6597..7184
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     polyA_signal    7369..7450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7406..7425)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7460..7479
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7527..7543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7527..7543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7540..7562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7551..7567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7575..7581,7582..7599,7600..7605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7620..7641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     1981 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2041 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2101 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     2161 gagcagattg tactgagagt gcaccataaa attgtaaacg ttaatatttt gttaaaattc
     2221 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     2281 ccttataaat caaaagaata gcccgagata gggttgagtg ttgttccagt ttggaacaag
     2341 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     2401 gatggcccac tacgtgaacc atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa
     2461 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     2521 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     2581 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     2641 gcgtactatg gttgctttga cgtatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     2701 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     2761 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     2821 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
     2881 tggccggcct aggcgcgcca agcttaaggt gcacggccca cgtggccact agtacttctc
     2941 gagctctgta catgtccgcg gtcgcgacgt acgcgtatct ttacatgtag cttgtgcatt
     3001 gaatccaatt ataatttgcc ttggcaccag ctgagccaga caagaaagaa agcttcccag
     3061 aagtatatcg atttagaagg gttgacgtca cttgctgact gcactaatac agcaaatgat
     3121 gcaattagaa tgattcaagt gaaattccca aattactgat ttttctctgg atttggttat
     3181 cagattacat tcgaagctaa gattagctac cgaaattgtc gatcaaatca ggaaatcctt
     3241 tctctatcga aaaaggcatt cgcacatctt cctctctatg ccatatacac gaagggtagg
     3301 tacattgacg tctttgccag aagttgaact gcatcgttca aggtacagaa tgaacgacta
     3361 acagacacaa gcacgttttg ctgtccattc agacacaggg atggtaccca tattcgatcg
     3421 atatagagcc atccaaccga acagaggtat atgtatgaat gtattgctga aattttctag
     3481 aagtacaacc accactacga cagtgtctat aaaacgcccc tgcaaaggcg aaaccagctc
     3541 aatcgaatac gtttcctagt ggagtgaaca ttacgcggcc caagtaagca gtgccagtgc
     3601 aagtgaagtg aagtctctag tgaaaaagag tgatccaatt agccagagga gaaaatttca
     3661 gagtgaacaa agctttattc aaaggacaat tactattaaa ttggtgaaag tgcatttcgg
     3721 tgaagggaat cttctagtga aggtaggtaa attaattgat gaaattatag ctatgagcga
     3781 aaactagttt ggtgaatgat tcctttgtct ttgaatgagc aaactatttt ccaagatggc
     3841 gactattgag ctttgagtga ttagtgaaaa tttgcaacgc agtttcatca tcattgataa
     3901 aacccaattg tgattcacag cgataatcat atttcgttga atcatcgcta ctaattgaat
     3961 taaatttcta gaataataag aataacgtat ttgctccgtc acatatctaa aataaatatt
     4021 ttgatggtaa ttacccatta aggtaatatt aacacatatc gagaaaaacc ttgaggaaat
     4081 cgtgaaaact tgaagatacg caatttcaaa actacgtagt tcaaagtcga aaacaagtta
     4141 atttttcact taaaagtagg gcgttgttgt gacgtcatca ccttcaagtg tatatttttc
     4201 acttggcctg cgactgcaaa cgcagacaaa gcaaaacaag tttaaaacct gtcgtgtcgt
     4261 gctcgaagcc aaaggcaatg aatcaatatc aaatgagagt ttgcatttca caaccaatta
     4321 ctcaagcgtt tcctcgtttc tttttctgct caacagagat ttcaaccatg ggcgatccta
     4381 aaaagaaacg taaggtcatc gataaggaga ccgccgctgc caagttcgag agacagcaca
     4441 tggacagcat cgatatcgcc gatctacgca cgctcggcta cagccagcag caacaggaga
     4501 agatcaaacc gaaggttcgt tcgacagtgg cgcagcacca cgaggcactg gtcggccacg
     4561 ggtttacaca cgcgcacatc gttgcgttaa gccaacaccc ggcagcgtta gggaccgtcg
     4621 ctgtcaagta tcaggacatg atcgcagcgt tgccagaggc gacacacgaa gcgatcgttg
     4681 gcgtcggcaa acagtggtcc ggcgcacgcg ctctggaggc cttgctcacg gtggcgggag
     4741 agttgagagg tccaccgtta cagttggaca caggccaact tctcaagatt gcaaaacgtg
     4801 gcggcgtgac cgcagtggag gcagtgcatg catggcgcaa tgcactgacg ggtgccccgc
     4861 tcaacttgac cccccagcag gtggtggcca tcgccagcaa taatggtggc aagcaggcgc
     4921 tggagacggt ccagcggctg ttgccggtgc tgtgccaggc ccacggcttg accccggagc
     4981 aggtggtggc catcgccagc aatattggtg gcaagcaggc gctggagacg gtgcaggcgc
     5041 tgttgccggt gctgtgccag gcccacggct tgacccccca gcaggtggtg gccatcgcca
     5101 gcaatggcgg tggcaagcag gcgctggaga cggtccagcg gctgttgccg gtgctgtgcc
     5161 aggcccacgg cttgaccccc cagcaggtgg tggccatcgc cagcaatggc ggtggcaagc
     5221 aggcgctgga gacggtccag cggctgttgc cggtgctgtg ccaggcccac ggcttgaccc
     5281 cccagcaggt ggtggccatc gccagcaata atggtggcaa gcaggcgctg gagacggtcc
     5341 agcggctgtt gccggtgctg tgccaggccc acggcttgac cccggagcag gtggtggcca
     5401 tcgccagcaa tattggtggc aagcaggcgc tggagacggt gcaggcgctg ttgccggtgc
     5461 tgtgccaggc ccacggcttg accccggagc aggtggtggc catcgccagc aatattggtg
     5521 gcaagcaggc gctggagacg gtgcaggcgc tgttgccggt gctgtgccag gcccacggct
     5581 tgaccccgga gcaggtggtg gccatcgcca gccacgatgg cggcaagcag gcgctggaga
     5641 cggtccagcg gctgttgccg gtgctgtgcc aggcccacgg cttgaccccg gagcaggtgg
     5701 tggccatcgc cagcaatatt ggtggcaagc aggcgctgga gacggtgcag gcgctgttgc
     5761 cggtgctgtg ccaggcccac ggcttgaccc cggagcaggt ggtggccatc gccagcaata
     5821 ttggtggcaa gcaggcgctg gagacggtgc aggcgctgtt gccggtgctg tgccaggccc
     5881 acggcttgac cccggagcag gtggtggcca tcgccagcca cgatggcggc aagcaggcgc
     5941 tggagacggt ccagcggctg ttgccggtgc tgtgccaggc ccacggcttg accccccagc
     6001 aggtggtggc catcgccagc aatggcggtg gcaagcaggc gctggagacg gtccagcggc
     6061 tgttgccggt gctgtgccag gcccacggct tgaccccgga gcaggtggtg gccatcgcca
     6121 gccacgatgg cggcaagcag gcgctggaga cggtccagcg gctgttgccg gtgctgtgcc
     6181 aggcccacgg cttgaccccc cagcaggtgg tggccatcgc cagcaataat ggtggcaagc
     6241 aggcgctgga gacggtccag cggctgttgc cggtgctgtg ccaggcccac ggcttgaccc
     6301 cccagcaggt ggtggccatc gccagcaata atggtggcaa gcaggcgctg gagacggtcc
     6361 agcggctgtt gccggtgctg tgccaggccc acggcttgac ccctcagcag gtggtggcca
     6421 tcgccagcaa tggcggcggc aggccggcgc tggagagcat tgttgcccag ttatctcgcc
     6481 ctgatccggc gttggccgcg ttgaccaacg accacctcgt cgccttggcc tgcctcggcg
     6541 ggcgtcctgc gctggatgca gtgaaaaagg gattggggga tcctatcagc cgttcccagc
     6601 tggtgaagtc cgagctggag gagaagaaat ccgagttgag gcacaagctg aagtacgtgc
     6661 cccacgagta catcgagctg atcgagatcg cccggaacag cacccaggac cgtatcctgg
     6721 agatgaaggt gatggagttc ttcatgaagg tgtacggcta caggggcaag cacctgggcg
     6781 gctccaggaa gcccgacggc gccatctaca ccgtgggctc ccccatcgac tacggcgtga
     6841 tcgtggacac caaggcctac tccggcggct acaacctgcc catcggccag gccgacgaaa
     6901 tgcagaggta cgtggaggag aaccagacca ggaacaagca catcaacccc aacgagtggt
     6961 ggaaggtgta cccctccagc gtgaccgagt tcaagttcct gttcgtgtcc ggccacttca
     7021 agggcaacta caaggcccag ctgaccaggc tgaaccacat caccaactgc aacggcgccg
     7081 tgctgtccgt ggaggagctc ctgatcggcg gcgagatgat caaggccggc accctgaccc
     7141 tggaggaggt gaggaggaag ttcaacaacg gcgagatcaa cttcgcggcc gactgataac
     7201 tcgagcgatc ctctagacga gctcctcgag cctgcagatc tgtcgacgcg gccgcgactc
     7261 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     7321 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     7381 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7441 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatcttg aattcggccg
     7501 gcctaggcgc gccaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     7561 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7621 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7681 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7741 gggcgc
//
