LOCUS       sequence-8229-ex        2710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Nashmi R, Dickinson ME, McKinney S, Jareb M, Labarca C, Fraser SE, 
            Lester HA
  TITLE     Assembly of alpha4beta2 nicotinic acetylcholine receptors assessed 
            with functional fluorescently labeled subunits: effects of 
            localization, trafficking, and nicotine-induced upregulation in 
            clonal mammalian cells and in cultured midbrain neurons.
  JOURNAL   J Neurosci. 2003 Dec 17. 23(37):11554-67.
  PUBMED    14684858
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2003 Dec 17. 23(37):11554-67."
COMMENT     Sequence Label: nAChR alpha4 CFP
FEATURES             Location/Qualifiers
     source          1..2710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1287..1316
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1317..1319,1320..1322,1323..2033)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1362..1383)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1623..1642)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1970..1991
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccatggagat cgggggctcc ggggcgccgc cgccgctgct gctcctgctg ctcctgctgc
       61 tcttagggac cggcctcttg cctgctagca gccacataga gacccgggcc catgcggagg
      121 agcggctcct gaagagactc ttctctggct acaacaagtg gtctcggcca gtagccaata
      181 tctcagatgt ggtccttgtc cgctttggct tgtcgattgc tcagctcatt gatgtggatg
      241 agaaaaacca gatgatgacg accaacgtgt gggtgaagca ggagtggcat gactacaaac
      301 tgcgctggga ccctggtgac tacgagaatg tcacctccat ccgcatccca tctgaactca
      361 tctggaggcc tgacatcgtc ctctacaaca acgcggacgg gaactttgca gtcacccacc
      421 taaccaaagc ccacctgttc tatgatgggc gtgtacagtg gacacccccg gccatctata
      481 agagctcctg cagcatcgac gtcaccttct tccccttcga ccagcagaac tgtaccatga
      541 agtttgggtc ctggacctac gacaaggcca agattgactt ggtgagcatg cacagccgtg
      601 tggaccaact ggacttctgg gaaagtgggg agtgggtcat tgtggatgcc gtgggcacct
      661 acaacaccag gaagtatgaa tgctgtgccg agatctatcc tgacatcacc tacgccttca
      721 tcatccgccg actgccactg ttctacacca tcaaccttat catcccgtgc ctgctcatct
      781 cctgcctcac cgtgctggtc ttctatctgc cctcggagtg cggcgagaag gtcacgctgt
      841 gcatctcggt gctgctttct ctcaccgtct tcctgctgct catcaccgag atcatcccgt
      901 ccacctcgct ggtcatcccg ctcatcggcg agtacctgct cttcaccatg atcttcgtca
      961 ccctctccat tgtcatcacg gtcttcgtgc tcaatgtaca ccaccgctca ccacgcacac
     1021 acaccatgcc cgcctgggtg cgcagagtct tcctggacat tgtgccccgt ctcctcttca
     1081 tgaagcgccc atctgtggtc aaagacaact gccggagact tatcgaatcc atgcacaaga
     1141 tggccaacgc ccctcgtttc tggccagagc ctgagagtga gcccggcatc ttgggtgaca
     1201 tctgcaacca aggcctgtca cctgccccaa ctttctgcaa ccgcatggac acagcagtcg
     1261 agacccagcc tacatgcagg tcacccgagc agaagctgat ctcagaggag gatctgatgg
     1321 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1381 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1441 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1501 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     1561 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1621 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1681 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1741 tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
     1801 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1861 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1921 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1981 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagaggtcac
     2041 cctcccacaa ggtccctgac ttgaagacat cagaggttga gaaggccagt ccctgtccat
     2101 cacctggctc ctgtcaccca cccaatagca gtggggcccc agtgctcatc aaagccaggt
     2161 ccctgagcgt ccagcatgtg cccagctccc aggaagcagc cgagggcagc atccgctgcc
     2221 ggtctcggag tatccagtac tgtgtttccc aagatggagc tgcttccctg actgagagca
     2281 agcccactgg ctccccagcc tccctgaaga cccgtccatc ccagcttcca gtgtcagacc
     2341 agacctctcc atgcaaatgc acatgcaagg aaccatctcc tgtgtccccc atcactgtgc
     2401 tcaaggctgg aggcaccaaa gcacctcccc aacacctgcc cctgtcacca gccctgacac
     2461 gggcagtaga aggcgtccag tacattgcag accacctcaa ggcagaagac acagacttct
     2521 cggtgaagga ggactggaaa tacgtggcca tggtcattga ccgaatcttc ctctggatgt
     2581 tcatcattgt ctgccttctg ggcactgtgg gactcttcct gcctccatgg ttggctggta
     2641 tgatctaggg aatagcggca cctagctccc aggtctctac agggccatgc gactcgtcag
     2701 tccacccaca
//
