LOCUS       sequence-82295-e        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses APEX2 in the cytosol of mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: pcDNA3 APEX2-NES
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    123..347
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     336..353
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        373..391
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     373..390
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(428..454)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
       61 gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga
      121 agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt
      181 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
      241 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
      301 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca
      361 gcgagctcta gcatttaggt gacactatag aatagggccc tctagatgca tgctcgagct
      421 attagtccag ggtcaggcgc tccaggggag gcagctgcag ggcatcagca aacccaagct
      481 cggaaagctt ttggtgagcc tcagcgtaat cagcaaagaa ggcatcttcg tccgctgcat
      541 atttgtcaac gagagggcgg aatacagggt cagacaaaag agccttgtca gaaggtagct
      601 gaaggagacc ttccttctca ccactcaaca actccgtgaa gtatgagttg tcgaaaataa
      661 gaggattaga ggtccaggga ccctcaaatc cagaacgctc cttgtgtgca gctccaatag
      721 tgtgaccccc agatagagca acgatatctt ggtcagtaag ccccatagct ttgccaaaca
      781 catctctcaa atggtcagaa cccttagtgg gatcgggcaa gcgaccctct ggtggtggct
      841 caggcttgtc ctctcttcca gggtggaatg gaaccttagg tccacccgtg acctcaacgg
      901 caacaacgcc agccaactgg tagaaatcgg cgtagctcaa aatagggaac tccgccttga
      961 gtggctccaa aagcctaaca gcgatgtcaa gaccgttgtt agcgctgtga gccagttcgg
     1021 cagggtgctt gatggttccg aagggtccac cggtcttcgt gcccttgtca aaggttccag
     1081 cagagtggaa tgccaaacgg agcattagag gagcgcatct cttctcag
//
