LOCUS       sequence-8230-ex        6600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Chih B, Gollan L, Scheiffele P
  TITLE     Alternative splicing controls selective trans-synaptic interactions 
            of the neuroligin-neurexin complex.
  JOURNAL   Neuron. 2006 Jul 20. 51(2):171-8.
  PUBMED    16846852
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2006 Jul 20. 51(2):171-8."
COMMENT     Sequence Label: pNICE-NL2(-)
FEATURES             Location/Qualifiers
     source          1..6600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             776..802
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3388..3509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3425..3444)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3479..3498
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3516..3971)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3653..3674)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3865..3884
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3998..4102
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4104..4461
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4104..4295
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4127..4147)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4312..4447
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4496..5290
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4496..5290
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4550..4569)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5160..5179
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5478..5497)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5522..5569
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5898..6486
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6387..6406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caaagcttgg gaccatggca cttcccagat gcatgtggcc
      661 aaattatgtt tggagagcta tgatggcatg tgtggtccac aggggatccg gtgccccatt
      721 gactctctgc ttgttgggat gtttgctaca gacttttcac gtactctctc aaaagtatcc
      781 atacgacgtt ccggactacg cacaacgggg aggagggggt cccggcggcg gcgccccggg
      841 cggcccaggc ctgggcctcg gcagcctcgg ggaggagcgc ttccccgtgg tgaacacagc
      901 ctacgggcga gtgcgcggtg tgcggcgcga gctcaacaac gagatcctgg gcccggtcgt
      961 gcagttcttg ggcgtgccct acgccacgcc gcccttgggc gcccgccgct tccagccgcc
     1021 tgaggcacct gcctcgtggc ccggcgtgcg caacgccacc accctgccgc ccgcctgccc
     1081 gcagaacctg cacggggccc tgccggccat catgctgcct gtgtggttca ccgacaactt
     1141 ggaggcggcc gccacctacg tgcagaacca gagcgaggac tgcctgtacc tcaacctcta
     1201 cgtgcccact gaggacgata tccgggactc tgggaagaaa ccggtcatgc tgtttctaca
     1261 cggcggctcc tacatggaag gcaccgggaa catgtttgac ggctcagtcc tggctgccta
     1321 tggcaatgtc atcgtagcca cactcaacta ccgtcttggg gtgctcggtt ttctcagcac
     1381 tggtgaccag gctgcaaaag gcaactatgg gctcctggac cagatccagg ccctgcgctg
     1441 gctcagtgaa aacattgccc actttggagg tgaccctgaa cgcatcacta tctttgggtc
     1501 tggtgcaggg gcctcctgtg tcaacttgct gatcctttcc caccactcag aaggactgtt
     1561 ccagaaggcc attgctcaaa gtggtactgc catttccagc tggtctgtca actaccagcc
     1621 gctcaagtac acgcggctgc tggcggccaa agtgggctgt gaccgagaag acagcactga
     1681 agctgtggag tgtctgcgcc ggaagtcttc ccgggagcta gtggaccagg atgtacagcc
     1741 tgcccgctac cacattgcct ttgggcctgt ggtggacggc gacgtagtcc ctgatgaccc
     1801 cgagatcctc atgcaacagg gggaattcct caactacgac atgctcattg gtgtcaacca
     1861 gggagagggt ctcaagttcg tggaggactc tgcagagagt gaggacggtg tgtctgccag
     1921 cgcctttgac ttcaccgtct ccaactttgt ggacaacttg tacgggtacc cagaaggcaa
     1981 ggacgtgctt cgagagacca tcaagttcat gtacacggac tgggctgaca gggacaatgg
     2041 cgagatgcgg cggaagaccc tgctggcgct ctttaccgac caccagtggg tcgccccggc
     2101 tgtggccacc gccaagctgc atgccgacta ccagtccccc gtctactttt acactttcta
     2161 ccaccactgc caggcagagg gccggccaga gtgggcagac gcagcgcacg gggacgagct
     2221 gccctacgtc tttggtgtcc ccatggtggg cgccactgac ctcttcccct gcaacttctc
     2281 caagaacgat gtcatgctca gcgcagtagt catgacctac tggaccaact tcgccaagac
     2341 tggtgacccc aaccagcctg tgccacagga caccaagttc atccacacca agcccaaccg
     2401 ctttgaagag gtagtgtgga gcaagttcaa cagcaaggaa aagcagtatc tgcacatagg
     2461 cttgaaacca cgcgtgcgcg acaactaccg tgccaacaag gtggccttct ggctggagct
     2521 cgtgccccac ctgcacaacc tgcacacaga gctcttcacc accaccactc gcctgcctcc
     2581 ctatgccaca cgctggccac ctcgcacacc tggtcctggc acttccggca cacgccgtcc
     2641 tcccccacct gccactctgc cacctgagtc tgatattgac ctaggcccaa gggcctatga
     2701 ccgcttcccc ggtgactcga gggactactc cacggagcta agcgtgactg tggcagtggg
     2761 tgcctccctc ctcttcctca acatccttgc ctttgccgcc ctctattaca agcgggaccg
     2821 gcgccaggag ctgcggtgcc ggaggcttag cccaccagga ggctcaggct caggtgtgcc
     2881 tggtgggggc cccctgcttc ccactgctgg ccgtgagcta cccccggagg aggagctagt
     2941 atcgctgcag ctgaagcggg gtggtggtgt tggggcggac cctgctgagg ccctgcgccc
     3001 tgcctgtcca cccgactata ccctggcctt gcgccgggca ccggacgatg tgcctctctt
     3061 ggcccccggg gccctaaccc tgctgcctag tggcctgggg cccccgcccc cgcccccacc
     3121 cccttctctc catccctttg ggcccttccc acccccaccc cctactgcta ccagccacaa
     3181 caacacgcta ccccatcccc actccaccac tcgggtatag ggggcggctt tgggaggtct
     3241 gcctccctaa ccctccaatc actagtgcgg ccgcgactct agatcataat cagccatacc
     3301 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     3361 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     3421 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     3481 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt
     3541 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     3601 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     3661 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     3721 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     3781 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     3841 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     3901 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     3961 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4021 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4081 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     4141 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4201 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4261 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     4321 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4381 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     4441 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     4501 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     4561 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     4621 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     4681 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     4741 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     4801 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     4861 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     4921 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4981 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     5041 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     5101 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     5161 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     5221 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     5281 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     5341 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     5401 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     5461 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     5521 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     5581 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     5641 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     5701 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     5761 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5821 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5881 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5941 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6001 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6061 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6121 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6181 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6241 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6301 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6361 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6421 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6481 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6541 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
