LOCUS       sequence-82300-e         532 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Lowney P, Corral J, Detmer K, LeBeau MM, Deaven L, Lawrence HJ, 
            Largman C
  TITLE     A human Hox 1 homeobox gene exhibits myeloid-specific expression of 
            alternative transcripts in human hematopoietic cells.
  JOURNAL   Nucleic Acids Res 1991 Jun 25;19(12):3443-9.
  PUBMED    1676505
REFERENCE   2  (bases 1 to 532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "1991-06-25"; volume: "19(12)"; pages: "3443-9"
COMMENT     Sequence Label: HOXA10 (human) bluescript
FEATURES             Location/Qualifiers
     source          1..532
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..230
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    347..368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(383..388,389..406,407..413)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    421..437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     426..448
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     445..461
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     445..461
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     480..500
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        482..500
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ccctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
       61 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      121 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
      181 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
      241 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
      301 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      361 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      421 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      481 caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc gg
//
