LOCUS       sequence-82307-e        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Envelope protein for producing lentiviral and MuLV retroviral 
            particles. Use in conjunction with a packaging vector such as 
            pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, 
            Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
  TITLE     Lentivirus-delivered stable gene silencing by RNAi in primary cells.
  JOURNAL   RNA 2003 Apr;9(4):493-501.
  PUBMED    12649500
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2003-04"; volume: "9(4)"; pages: "493-501"
COMMENT     Sequence Label: pCMV-VSV-G
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        104..483
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        484..687
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     638..658
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     684..708
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cgcggtggcg gccgctctag agagcttggc ccattgcata cgttgtatcc atatcataat
       61 atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg attattgact
      121 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      181 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      241 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      301 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      361 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      421 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      481 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      541 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      601 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      661 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      721 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggtcgacc
      781 gatcctgaga acttcagggt gagtttgggg acccttgatt gttctttctt tttcgctatt
      841 gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag aatgggaaga
      901 tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt tctactctgt
      961 tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc gttaaacttt
     1021 agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga ttgtaagtac
     1081 tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac ttc
//
