LOCUS       sequence-82308-e        6142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Aedes aegypti polyubiquitin promoter driving firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Anderson MA, Gross TL, Myles KM, Adelman ZN
  TITLE     Validation of novel promoter sequences derived from two endogenous 
            ubiquitin genes in transgenic Aedes aegypti.
  JOURNAL   Insect Mol Biol. 2010 Aug;19(4):441-9. doi: 
            10.1111/j.1365-2583.2010.01005.x. Epub 2010 Apr 26.
  PUBMED    20456509
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2583.2010.01005.x"; journalName: "Insect Mol Biol"; 
            date: "2010-08"; volume: "19"; issue: "4"; pages: "441-9"
COMMENT     Sequence Label: pGL3-PUb
FEATURES             Location/Qualifiers
     source          1..6142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1412..3064
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1478..1496)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3105..3226
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3116..3135)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3170..3189
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3474..3491)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3645..4233)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3725..3744)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4404..5195,5196..5264))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5027..5046
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5265..5369)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5396..5851
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5483..5502)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5693..5714
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5982..6030
                     /note="synthetic polyadenylation signal"
     misc_feature    6044..6135
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6084..6103
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt atctttacat gtagcttgtg cattgaatcc aattataatt
       61 tgccttggca ccagctgagc cagacaagaa agaaagcttc ccagaagtat atcgatttag
      121 aagggttgac gtcacttgct gactgcacta atacagcaaa tgatgcaatt agaatgattc
      181 aagtgaaatt cccaaattac tgatttttct ctggatttgg ttatcagatt acattcgaag
      241 ctaagattag ctaccgaaat tgtcgatcaa atcaggaaat cctttctcta tcgaaaaagg
      301 cattcgcaca tcttcctctc tatgccatat acacgaaggg taggtacatt gacgtctttg
      361 ccagaagttg aactgcatcg ttcaaggtac agaatgaacg actaacagac acaagcacgt
      421 tttgctgtcc attcagacac agggatggta cccatattcg atcgatatag agccatccaa
      481 ccgaacagag gtatatgtat gaatgtattg ctgaaatttt ctagaagtac aaccaccact
      541 acgacagtgt ctataaaacg cccctgcaaa ggcgaaacca gctcaatcga atacgtttcc
      601 tagtggagtg aacattacgc ggcccaagta agcagtgcca gtgcaagtga agtgaagtct
      661 ctagtgaaaa agagtgatcc aattagccag aggagaaaat ttcagagtga acaaagcttt
      721 attcaaagga caattactat taaattggtg aaagtgcatt tcggtgaagg gaatcttcta
      781 gtgaaggtag gtaaattaat tgatgaaatt atagctatga gcgaaaacta gtttggtgaa
      841 tgattccttt gtctttgaat gagcaaacta ttttccaaga tggcgactat tgagctttga
      901 gtgattagtg aaaatttgca acgcagtttc atcatcattg ataaaaccca attgtgattc
      961 acagcgataa tcatatttcg ttgaatcatc gctactaatt gaattaaatt tctagaataa
     1021 taagaataac gtatttgctc cgtcacatat ctaaaataaa tattttgatg gtaattaccc
     1081 attaaggtaa tattaacaca tatcgagaaa aaccttgagg aaatcgtgaa aacttgaaga
     1141 tacgcaattt caaaactacg tagttcaaag tcgaaaacaa gttaattttt cacttaaaag
     1201 tagggcgttg ttgtgacgtc atcaccttca agtgtatatt tttcacttgg cctgcgactg
     1261 caaacgcaga caaagcaaaa caagtttaaa acctgtcgtg tcgtgctcga agccaaaggc
     1321 aatgaatcaa tatcaaatga gagtttgcat ttcacaacca attactcaag cgtttcctcg
     1381 tttctttttc tgctcaacag agatttcaac catggaagac gccaaaaaca taaagaaagg
     1441 cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat
     1501 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga
     1561 catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
     1621 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
     1681 gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa
     1741 tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa
     1801 aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat
     1861 tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc
     1921 tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa
     1981 gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct
     2041 gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca
     2101 aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt
     2161 tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga
     2221 agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac
     2281 cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca
     2341 cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag
     2401 gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct
     2461 gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga
     2521 agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact
     2581 gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc
     2641 cttgattgac aaggatggat ggctacattc tggagacata gcttactggg acgaagacga
     2701 acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc
     2761 tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg caggtgtcgc
     2821 aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg
     2881 aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa
     2941 aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact
     3001 cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt
     3061 gtaattctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     3121 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     3181 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     3241 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     3301 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
     3361 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     3421 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
     3481 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3541 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3601 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3661 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3721 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3781 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3841 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3901 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3961 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4021 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4081 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4141 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4201 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4261 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4321 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4381 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4441 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4501 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4561 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4621 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4681 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4741 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4801 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4861 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4921 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4981 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5041 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5101 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5161 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5221 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5281 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5341 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     5401 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5461 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5521 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5581 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5641 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5701 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5761 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5821 aattttaaca aaatattaac gcttacaatt tgccattcgc cattcaggct gcgcaactgt
     5881 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct accatgataa
     5941 gtaagtaata ttaaggtacg ggaggtactt ggagcggccg caataaaata tctttatttt
     6001 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     6061 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     6121 ccagaacatt tctctatcga ta
//
