LOCUS       sequence-8231-ex        6651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Chih B, Gollan L, Scheiffele P
  TITLE     Alternative splicing controls selective trans-synaptic interactions 
            of the neuroligin-neurexin complex.
  JOURNAL   Neuron. 2006 Jul 20. 51(2):171-8.
  PUBMED    16846852
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2006 Jul 20. 51(2):171-8."
COMMENT     Sequence Label: pNICE-NL2A
FEATURES             Location/Qualifiers
     source          1..6651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             776..802
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3439..3560
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3476..3495)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3530..3549
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3567..4022)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3704..3725)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3916..3935
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4049..4153
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4155..4512
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4155..4346
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4178..4198)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4363..4498
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4547..5341
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4547..5341
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4601..4620)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5211..5230
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5529..5548)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5573..5620
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5949..6537
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6438..6457
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caaagcttgg gaccatggca cttcccagat gcatgtggcc
      661 aaattatgtt tggagagcta tgatggcatg tgtggtccac aggggatccg gtgccccatt
      721 gactctctgc ttgttgggat gtttgctaca gacttttcac gtactctctc aaaagtatcc
      781 atacgacgtt ccggactacg cacaacgggg aggagggggt cccggcggcg gcgccccggg
      841 cggcccaggc ctgggcctcg gcagcctcgg ggaggagcgc ttccccgtgg tgaacacagc
      901 ctacgggcga gtgcgcggtg tgcggcgcga gctcaacaac gagatcctgg gcccggtcgt
      961 gcagttcttg ggcgtgccct acgccacgcc gcccttgggc gcccgccgct tccagccgcc
     1021 tgaggcacct gcctcgtggc ccggcgtgcg caacgccacc accctgccgc ccgcctgccc
     1081 gcagaacctg cacggggccc tgccggccat catgctgcct gtgtggttca ccgacaactt
     1141 ggaggcggcc gccacctacg tgcagaacca gagcgaggac tgcctgtacc tcaacctcta
     1201 cgtgcccact gaggacggtc cgctcacaaa aaaacgtgac gaggcgacgc tcaatccgcc
     1261 agacacagat atccgggact ctgggaagaa accggtcatg ctgtttctac acggcggctc
     1321 ctacatggaa ggcaccggga acatgtttga cggctcagtc ctggctgcct atggcaatgt
     1381 catcgtagcc acactcaact accgtcttgg ggtgctcggt tttctcagca ctggtgacca
     1441 ggctgcaaaa ggcaactatg ggctcctgga ccagatccag gccctgcgct ggctcagtga
     1501 aaacattgcc cactttggag gtgaccctga acgcatcact atctttgggt ctggtgcagg
     1561 ggcctcctgt gtcaacttgc tgatcctttc ccaccactca gaaggactgt tccagaaggc
     1621 cattgctcaa agtggtactg ccatttccag ctggtctgtc aactaccagc cgctcaagta
     1681 cacgcggctg ctggcggcca aagtgggctg tgaccgagaa gacagcactg aagctgtgga
     1741 gtgtctgcgc cggaagtctt cccgggagct agtggaccag gatgtacagc ctgcccgcta
     1801 ccacattgcc tttgggcctg tggtggacgg cgacgtagtc cctgatgacc ccgagatcct
     1861 catgcaacag ggggaattcc tcaactacga catgctcatt ggtgtcaacc agggagaggg
     1921 tctcaagttc gtggaggact ctgcagagag tgaggacggt gtgtctgcca gcgcctttga
     1981 cttcaccgtc tccaactttg tggacaactt gtacgggtac ccagaaggca aggacgtgct
     2041 tcgagagacc atcaagttca tgtacacgga ctgggctgac agggacaatg gcgagatgcg
     2101 gcggaagacc ctgctggcgc tctttaccga ccaccagtgg gtcgccccgg ctgtggccac
     2161 cgccaagctg catgccgact accagtcccc cgtctacttt tacactttct accaccactg
     2221 ccaggcagag ggccggccag agtgggcaga cgcagcgcac ggggacgagc tgccctacgt
     2281 ctttggtgtc cccatggtgg gcgccactga cctcttcccc tgcaacttct ccaagaacga
     2341 tgtcatgctc agcgcagtag tcatgaccta ctggaccaac ttcgccaaga ctggtgaccc
     2401 caaccagcct gtgccacagg acaccaagtt catccacacc aagcccaacc gctttgaaga
     2461 ggtagtgtgg agcaagttca acagcaagga aaagcagtat ctgcacatag gcttgaaacc
     2521 acgcgtgcgc gacaactacc gtgccaacaa ggtggccttc tggctggagc tcgtgcccca
     2581 cctgcacaac ctgcacacag agctcttcac caccaccact cgcctgcctc cctatgccac
     2641 acgctggcca cctcgcacac ctggtcctgg cacttccggc acacgccgtc ctcccccacc
     2701 tgccactctg ccacctgagt ctgatattga cctaggccca agggcctatg accgcttccc
     2761 cggtgactcg agggactact ccacggagct aagcgtgact gtggcagtgg gtgcctccct
     2821 cctcttcctc aacatccttg cctttgccgc cctctattac aagcgggacc ggcgccagga
     2881 gctgcggtgc cggaggctta gcccaccagg aggctcaggc tcaggtgtgc ctggtggggg
     2941 ccccctgctt cccactgctg gccgtgagct acccccggag gaggagctag tatcgctgca
     3001 gctgaagcgg ggtggtggtg ttggggcgga ccctgctgag gccctgcgcc ctgcctgtcc
     3061 acccgactat accctggcct tgcgccgggc accggacgat gtgcctctct tggcccccgg
     3121 ggccctaacc ctgctgccta gtggcctggg gcccccgccc ccgcccccac ccccttctct
     3181 ccatcccttt gggcccttcc cacccccacc ccctactgct accagccaca acaacacgct
     3241 accccatccc cactccacca ctcgggtata gggggcggct ttgggaggtc tgcctcccta
     3301 accctccaat cactagtgcg gccgcgactc tagatcataa tcagccatac cacatttgta
     3361 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3421 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3481 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3541 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     3601 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     3661 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     3721 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     3781 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     3841 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     3901 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     3961 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     4021 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4081 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4141 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     4201 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4261 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4321 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4381 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4441 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4501 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     4561 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4621 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4681 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     4741 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4801 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4861 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4921 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4981 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     5041 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     5101 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     5161 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5221 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5281 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5341 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     5401 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5461 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     5521 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     5581 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     5641 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     5701 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     5761 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     5821 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5881 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5941 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6001 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6061 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     6121 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6181 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6301 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6481 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6541 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6601 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
