LOCUS       sequence-82310-e        5190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Aedes aegypti ubiquitin promoter driving firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Anderson MA, Gross TL, Myles KM, Adelman ZN
  TITLE     Validation of novel promoter sequences derived from two endogenous 
            ubiquitin genes in transgenic Aedes aegypti.
  JOURNAL   Insect Mol Biol. 2010 Aug;19(4):441-9. doi: 
            10.1111/j.1365-2583.2010.01005.x. Epub 2010 Apr 26.
  PUBMED    20456509
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2583.2010.01005.x"; journalName: "Insect Mol Biol"; 
            date: "2010-08"; volume: "19"; issue: "4"; pages: "441-9"
COMMENT     Sequence Label: pGL3-Ub(L40)
FEATURES             Location/Qualifiers
     source          1..5190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             460..2112
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(526..544)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2153..2274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2164..2183)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2218..2237
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2522..2539)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2693..3281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2773..2792)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3452..4243,4244..4312))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4075..4094
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4313..4417)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4444..4899
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4531..4550)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4741..4762
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5030..5078
                     /note="synthetic polyadenylation signal"
     misc_feature    5092..5183
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5132..5151
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt acggcgcgcc aagcttagcg tgaaccttgc atgcgtgcac
       61 agaatttgat tgcatttcat gaccgggtat ccattcggcg ccggccactg aactgaaatt
      121 cagttttgac agctccactg acttgaagct ctttttcacc gaaacacatc tcggaagaga
      181 cacgttggga aagagtaagt ttaatttttg tcgtttttcg tggattttta gtgataattc
      241 gtggtttttc gttgttagca atctaaatca ttgatagatc aagttagaaa ttgtgaaata
      301 ttgctaaaat acctacatta ttcgtagttt attcgatttg ttgtagttga tgcattctaa
      361 cctcaatgtg gtttcgtctc cgtccaattc cgatgttatt gtccagttcg ttaatttttg
      421 gtgttttctc cccgactttt ccacagactg atccaagcca tggaagacgc caaaaacata
      481 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
      541 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
      601 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
      661 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
      721 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
      781 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
      841 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa
      901 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
      961 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat
     1021 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt
     1081 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt
     1141 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
     1201 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
     1261 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg
     1321 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct
     1381 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt
     1441 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca
     1501 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
     1561 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga
     1621 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg
     1681 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
     1741 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat
     1801 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca
     1861 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
     1921 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
     1981 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
     2041 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
     2101 atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac
     2161 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     2221 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     2281 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     2341 aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga tgcccttgag
     2401 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     2461 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt
     2521 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2581 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2641 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2701 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2761 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2821 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2881 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2941 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3001 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3061 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3121 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3181 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3241 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3301 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3361 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3421 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3481 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3541 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3601 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3661 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3721 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3781 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3841 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3901 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3961 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4021 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4081 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4141 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4201 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4261 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4321 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4381 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4441 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4501 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4561 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4621 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4681 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4741 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4801 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4861 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc
     4921 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac
     4981 catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc
     5041 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
     5101 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
     5161 tgcaggtgcc agaacatttc tctatcgata
//
