LOCUS       sequence-82317-e        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Jin J, Ang XL, Shirogane T, Wade Harper J
  TITLE     Identification of substrates for F-box proteins.
  JOURNAL   Methods Enzymol. 2005;399:287-309. doi: 
            10.1016/S0076-6879(05)99020-4.
  PUBMED    16338364
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2005"; volume: "399:287-309. doi"; pages: " 
            10.1016/S0076-6879(05)99020-4"
COMMENT     Sequence Label: pcDNA3-DN-hCUL1-FLAG
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc accatgtcgt
      121 caacccggag ccagaacccc cacggcctga agcagattgg cctggaccag atctgggacg
      181 acctcagagc cggcatccag caggtgtaca cacggcagag catggccaag tccagatata
      241 tggagctcta cactcatgtt tataactact gtactagtgt tcaccagtca aaccaagcac
      301 gaggagctgg agttcctcct tctaagtcga aaaaggggca gacacctgga ggagctcagt
      361 ttgttggcct ggaattatat aaacgactta aggaattttt gaagaattac ttgacaaatc
      421 ttcttaagga tggagaagat ttgatggatg agagtgtact gaaattctac actcaacaat
      481 gggaagatta tcgattttca agcaaagtgc tgaatggaat ttgtgcctac ctcaatagac
      541 attgggttcg ccgtgaatgt gacgaaggac gaaaaggaat atatgaaatc tattcgcttg
      601 cattggtgac ttggagagac tgtctgttca ggccactgaa taaacaggta acaaatgctg
      661 ttttaaagct gattgaaaag gaaaggaatg gtgaaaccat caatacaaga ttgattagtg
      721 gagttgtaca gtcttacgtg gaattggggc tgaatgaaga tgatgcattt gcaaagggcc
      781 ctacgttaac agtgtataaa gaatcctttg aatctcaatt tttggctgac acagagagat
      841 tttataccag agagagtact gaattcttgc agcagaaccc agttactgaa tatatgaaaa
      901 aggcagaggc tcgtctgctt gaggaacaac gaagagttca ggtttacctt catgaaagca
      961 cacaagatga attagcaagg aaatgtgaac aagtcctcat tgaaaaacac ttggaaattt
     1021 tccacacaga atttcagaat ttattggatg ctgacaaaaa t
//
