LOCUS       sequence-8232-ex        7393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Chih B, Gollan L, Scheiffele P
  TITLE     Alternative splicing controls selective trans-synaptic interactions 
            of the neuroligin-neurexin complex.
  JOURNAL   Neuron. 2006 Jul 20. 51(2):171-8.
  PUBMED    16846852
REFERENCE   2  (bases 1 to 7393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2006 Jul 20. 51(2):171-8."
COMMENT     Sequence Label: pCAG-NL1(-)
FEATURES             Location/Qualifiers
     source          1..7393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        368..645
                     /label=chicken beta-actin promoter
     intron          647..1664
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1588..1610
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1672..1691
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1910..1936
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      2332..2341
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4275..4284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4397..4416)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4462..4517
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4516..4535)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4878..4894)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4878..4894)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4891..4913)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4902..4918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4926..4932,4933..4950,4951..4956))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4971..4992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5051..5246
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5097..5232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5252..5386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5289..5308)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5343..5362
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5454..5471)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5625..6213)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5705..5724)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6384..7175,7176..7244))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7007..7026
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7245..7349)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(7380..7393,1..366)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      481 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      541 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac
      661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
      721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
      781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
      841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
      901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
      961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1681 tggttattgt gctgtctcat cattttggca aagaattcgc ggccgctagc gctaccggac
     1741 tcagatctcg agctcaaagc ttgggaccat ggcacttccc agatgcatgt ggccaaatta
     1801 tgtttggaga gctatgatgg catgtgtggt ccacagggga tccggtgccc cattgactct
     1861 ctgcttgttg ggatgtttgc tacagacttt tcacgtactc tctcaaaagt atccatacga
     1921 cgttccggac tacgcattgg atgatgtaga cccattggtt actactaact ttggcaagat
     1981 taggggaatt aagaaagaac tcaataatga aattttgggt cctgtcattc agtttcttgg
     2041 ggttccatat gccgctccac caacaggaga acatcgtttc cagcctccag aaccaccatc
     2101 tccctggtct gacatccgga acgccactca gtttgctcct gtatgtcccc agaatatcat
     2161 tgatggcaga ttgcctgaag ttatgcttcc tgtgtggttc actaataact tggatgtggt
     2221 ttcatcatac gtccaagacc agagtgaaga ctgtctatac ttaaacatct atgtcccaac
     2281 tgaagatgat attcgggaca gtgggggtcc caaaccagtg atggtgtaca tccatggcgg
     2341 ctcttacatg gaaggtactg gaaatctgta tgatgggagt gtcttggcaa gctatggcaa
     2401 tgtgatcgtc atcacagtca actatcggct tggggtactt ggcttcttga gcacagggga
     2461 tcaggctgcc aaaggaaact acgggctcct tgacctcatc caggccctaa gatggaccag
     2521 cgagaacatt gggttctttg gtggtgaccc cttgcgaatc actgtgtttg gatcaggcgc
     2581 tgggggttca tgtgtcaacc tgctgacttt atcccattat tctgaaggac tttttcaacg
     2641 agcaatagct cagagtggaa cagccctttc cagctgggct gttagtttcc agcctgcaaa
     2701 atacgctaga attctggcca caaaagttgg ctgcaatgtt tcagatacag tagagttagt
     2761 agaatgcctg cagaagaagc cttacaaaga acttgttgat caagatgttc aaccagcccg
     2821 ataccacata gcctttggac ctgtgatcga tggtgatgta ataccagatg accctcagat
     2881 actgatggaa caaggagagt tcctcaacta tgatataatg ttgggagtta accaagggga
     2941 agggttgaag tttgtcgaaa atatagtaga tagtgatgat ggtgtatcag ccagtgattt
     3001 cgactttgct gtttctaatt ttgttgataa tttatatgga tatcctgaag gcaaagatgt
     3061 tttgagagaa accattaaat tcatgtatac tgactgggct gatcgccata atcctgaaac
     3121 tagaaggaag acattgttgg ctttgtttac ggaccatcaa tgggtagcac ctgctgtggc
     3181 cacagcagac cttcactcga actttggctc acctacatac ttctatgcct tttatcatca
     3241 ttgccaaaca gatcaagttc cagcttgggc tgatgcagct catggggatg aggttcccta
     3301 tgtgttggga atccccatga ttggccctac agagttattt ccttgcaatt tctccaagaa
     3361 tgatgtgatg ttgagtgcag tagtaatgac atactggacg aattttgcta aaactggtga
     3421 cccaaatcaa ccagttcctc aagacacaaa attcatccat accaaaccca accgctttga
     3481 agaagtagca tggaccagat attcccagaa agaccagctt tatctccata ttggattaaa
     3541 accgagagtt aaagagcatt acagagccaa taaggtaaat ctctggctgg agctggtacc
     3601 tcatctgcat aatctcaatg acatttctca gtatacctcg acaacaacta aagtgccatc
     3661 cacggacatc actctcagac ctacaaggaa gaattccaca ccagtcacat cagcctttcc
     3721 cactgccaaa caggatgatc ccaagcaaca accaagcccc ttctcggtgg atcagaggga
     3781 ctactccaca gagctaagtg tcactatcgc agtgggggcc tctctgctgt ttctcaacat
     3841 cttggctttt gcagccctgt actacaagaa ggataagagg agacatgatg tccaccggag
     3901 gtgcagccct cagcgcacga ccaccaacga cctaacccat gctccagaag aggaaattat
     3961 gtctctccaa atgaagcaca ctgacttgga tcacgagtgt gagtccatcc atccacatga
     4021 ggtggttctt cggaccgcct gtcccccaga ttatactcta gctatgagga ggtcacctga
     4081 tgatattcca ctaatgacac ctaacaccat cacaatgatt cccaacacta taccagggat
     4141 tcagccctta catacattca acacatttac tggaggacag aataatacac tgccccatcc
     4201 ccacccacac ccccattcac attcaacaac cagggtatag ccagatgaga gagacaaact
     4261 gattaatccc gcggccatgg cggccgggag catgcgacgt cgggcccggg gagctcttca
     4321 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     4381 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     4441 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     4501 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     4561 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     4621 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     4681 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     4741 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     4801 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
     4861 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     4921 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     4981 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5041 cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5101 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5161 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     5221 tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta
     5281 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5341 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga
     5401 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5461 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5521 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5581 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5641 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5701 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5761 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5821 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5881 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5941 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6001 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6061 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6121 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6181 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6241 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6301 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6361 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6421 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6481 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6541 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6601 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6661 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6721 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6781 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6841 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6901 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6961 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7021 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7081 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7141 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7201 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7261 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7321 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg
     7381 acattgatta ttg
//
