LOCUS       sequence-82320-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of Large T Antigen. Used to create 
            immortalized cell lines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM, 
            DeCaprio JA, Weinberg RA
  TITLE     Enumeration of the simian virus 40 early region elements necessary 
            for human cell transformation.
  JOURNAL   Mol Cell Biol 2002 Apr;22(7):2111-23.
  PUBMED    11884599
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "2002-04"; volume: "22(7)"; pages: "2111-23"
COMMENT     Sequence Label: pBABE-neo largeTcDNA
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(58..77)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(711..730)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
       61 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      121 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      181 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      241 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      301 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
      361 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
      421 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
      481 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
      541 ttacgcgtaa ccaccacacc cgccgcatcg atgcatcgat gcgaacccca gagtcccgct
      601 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
      661 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
      721 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
      781 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
      841 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
      901 agcccctgat gctcttcgtc cagatcatcc tgatcg
//
