LOCUS       sequence-82324-e        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Sancak Y, Bar-Peled L, Zoncu R, Markhard AL, Nada S, Sabatini DM
  TITLE     Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is
            necessary for its activation by amino acids.
  JOURNAL   Cell. 2010 Apr 16. 141(2):290-303.
  PUBMED    20381137
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 16. 141(2):290-303."
COMMENT     Sequence Label: pLJM1 Flag Raptor Rheb15
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..80
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtatggact
       61 acaaggatga cgatgacaag ggtggtggtg gtggtgcgtc gactatggag tccgaaatgc
      121 tgcaatcgcc tcttctgggc ctgggggagg aagatgaggc tgatcttaca gactggaacc
      181 tacctttggc ttttatgaaa aagaggcact gtgagaaaat tgaaggctcc aaatccttag
      241 ctcagagctg gaggatgaag gatcggatga agacagtcag tgttgcctta gttttgtgcc
      301 tgaatgttgg tgtggaccct cccgatgtgg tgaagaccac gccctgtgca cgcttggaat
      361 gctggatcga tcctctgtcg atgggtcctc agaaagctct ggaaaccatc ggtgcaaatt
      421 tacagaagca gtacgagaac tggcagccaa gggcccggta caagcagagc cttgacccaa
      481 ctgtggatga agtcaagaag ctctgcacgt ccttacgtcg caacgccaag gaggagcgag
      541 tcctctttca ctacaatggc cacggggtgc cccggcccac agtcaacggg gaggtctggg
      601 tcttcaacaa gaactacacg cagtacatcc ctctgtccat atatgacctg cagacgtgga
      661 tgggcagccc gtcgatcttc gtctacgact gctccaatgc tggcttgatc gtcaagtcct
      721 tcaagcagtt cgcactacag cgggagcagg agctggaggt agctgcaatc aacccaaatc
      781 accctcttgc tcagatgcct ttgcctccgt cgatgaaaaa ctgcatccag ctggcagcct
      841 gcgaggccac cgagctgctg cccatgatcc ccgacctccc ggctgaccta ttcacctcct
      901 gcctcaccac ccccatcaag atcgccctgc gctggttttg catgcagaaa tgtgtcagtc
      961 tggtgcctgg cgtcacactg gatttgatag aaaagatccc tggccgcctg aacgacagga
     1021 ggacgcccct gggtgaactg aactggatct tcacagccat cacagacacc atcgcgt
//
