LOCUS       sequence-82331-e       12626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of FRT5-flanked Neo; homologous recombination targeting 
            vector cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12626)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 12626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: 
            pBT310_pBS-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..12626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(143..164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     355..374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    742..966
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     955..972
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(999..1019)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1020..2834)
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     regulatory      2831..2840
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             7856..7864
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    8434..8481
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     8506..8525
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(8713..9507)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(8713..9507)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8824..8843)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9434..9453
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(9542..9899)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9556..9691
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        9708..9899
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     9856..9876
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(9901..10005)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    10175..10274
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     promoter        complement(10441..10459)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10480..10496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10480..10496)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10493..10515)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10504..10520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10528..10534,10535..10552,10553..10558))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10573..10594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10711..10728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10882..11470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10962..10981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11641..12432,12433..12501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12264..12283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12502..12606)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacagg
      661 cgcgcccctt actaggcggc cgcgaagttc ctataccttt tgaagaatag gaacttcgga
      721 ataggaactt ctctaggacg tccatagagc ccaccgcatc cccagcatgc ctgctattgt
      781 cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc
      841 tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac
      901 cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag
      961 gcacagtcga ggctgatcag cgagctctag atctatcaca ctttccgctt tttcttaggg
     1021 gcggccacgt cctcggtgcc gtcctgggcg tcatcctcgt cgtcgtcggt gggaggcttg
     1081 gcccaggtga tgctggctct cttctcgatg ggggtgccct ggcccctgcc ggtggtgctc
     1141 ttggtcacca cgatcttatc cacgaacagg cccacgaaca ccctcttgtc gtccacgctg
     1201 gctctgcccc accaggactt ggggccggtg gggtcggcgt cggcatcctc ggggaaccac
     1261 tgatccaggg gcagcttagg ggcctcggcg gcctccagct cggccagtct ttcctcggcg
     1321 ccctgctgtc tcagggtcag ggcggcctgc tgctttctga agtgcttcct gcccacaggg
     1381 ccgtcatagg ctccggcggc tctgtcctcg tacagctcct ccagggcgtt cagggcgtcg
     1441 gctctctcgg ccaccaggtt ggccctctcg ccgctcttct cgggggcctc ggtcagcttg
     1501 ccgaatcttc tggcggcctc ccacagcagg gccagggtct cctcgtcgcc ctcggcgtgc
     1561 cggatcttgt tgaagatccg ctcggccacg aacttgtcca gggcggccat gctcacatta
     1621 caggtgccct cgtgctggcc aggggcgctg gggtccacca cctttcttct ccggcaccgg
     1681 tagctgtcct tgatgctctc ctcgcctctc ttgctggtca tcacggcgcc acactcacag
     1741 tacagcttgt ccatggcgct caggatggcc tggcctctgc tcaggccctt gcctctgcct
     1801 ctgccgtcca gccaggcctg cagctcgtac cactcggcag gctcgatgat agggccacag
     1861 tccagctcca caggtctcag ggtgatgggg tctctctgga ttctgtagcc ctcgatcttg
     1921 gtggtggggg tgccgtcggg cttcttcttg tagatcacct cggcggcgaa gccggcgatc
     1981 ctagggtccc tcaggattct catcacggtg gcggggtccc aggcgctgct ggcggttttc
     2041 ttgccgatgg tctcgcctct ggtgggcacg gcgtcggcgt ccattctctt acacaggccg
     2101 gtgatgctgc cggggtggat ggcggcctgg ctgccgggct tgaaaggcag gtgcttgtgg
     2161 gtcttgatct cccgccacca ccacctgatc acgtcgggct caaactcgaa ggggccggtc
     2221 aggggggtgg tgctgtgggc cagcttgttg atcaccacgt tcaccatcct gccgttccgg
     2281 gtgatctcct tggtctcgga caccagctcg aagccgtagg gggccttgcc gcccacatag
     2341 ccgcccagct ccctctgcag gttcttggtg tccaggatct tggcgctctt caggctgctc
     2401 tccttgtggc tggcgtccag cctcatgatc aggtggatca ggtccatcac gttgccctgt
     2461 ctaaagacgc cttcctgggt ggacacgatg gtcacgccca gggccagcag ctcggacacg
     2521 atagggatgg cgtccatcac cttcagcctg ctgaaccggg acacgtcgta cacgatgatc
     2581 atgttcagcc tgccggccct acactcgttc aggattctct cgaactcggg tctctcggcg
     2641 gtgccgaagg cgctggtgcc aggggcctcg ctgaagtggc ccacaaatct gaatctgccg
     2701 ccgtccctct ccacctctct ctgcagatcg gcggccttat cctcgttggc gcttctctgg
     2761 gtggcggggc tggcggcgct gctgttctct ctctcccggc tctgtctgtc gtaggctccg
     2821 gcgtaggtat ccatggtggc ggttaactct ccgctccagg ctccccgggc tcctcgcggc
     2881 cggccgacgc ggcttaaacg gctgcacgtg cgggtggacg ccggacgccg actgcgtcat
     2941 tggcgcgggg acaacaaata gcatcaggcg accgggcccg ccagccaatc agcggccgcg
     3001 gcgcgcgact tcccgcccca tggacggacg gcctgcgcgt tctccacctt tgttgaggcc
     3061 gaggccgagg ccgaggcagg gaggcctctt ggctccatgg cctgtgaggc ctgtgggagt
     3121 gagctgatcc ccggggctgc cacgcacgac atcccgtttc actctccaag ggctcatcaa
     3181 ttccattgtg ttcattcaaa agttaatata tatataaata aaaataaaaa tctggggctc
     3241 ggggaggcag aggcaggcaa atccctgggt ggaggccagc ctggtctaca gagcaaactt
     3301 caggacagtc agggctacac agagaaaccc tgtctggaga aaaaccaaag gggtggaaca
     3361 tctaattgct ttctctccca gccgttccag cctcctcttg acctcccaaa gtgccaggct
     3421 tggccattct gagtccgaac gtctcaagtg ctctctttgt atctgagaaa tgcacttcac
     3481 tcctctacta aaaccaacta gaagcaatgc ttctcctctc cataaaaagg gcaaggatga
     3541 gactccatct tgaagttgtc aagaaagcca gtctgtaaaa agaaaaccga agagaggaag
     3601 acttggtctg tctgccaagg cctggatttg tggtgtacct gtttacaatt ctgagggcac
     3661 cggccagttc agctcattaa gtagatgttc acattctgcc tgctcctcac ctctttctag
     3721 aaggaaaatt ccatccgtgg ttttcctagt acccgccaaa gggatcacaa tcaaatatat
     3781 gtaaagcatg ttcttgtttt tattttcact tccatcgctt tcctttccct ggatttttga
     3841 agaagttcta gatatctcag tctggaactt aaagatgagg aactccagct ttctagagcc
     3901 acctttctgt ttcagcacca aaaacacact agcatttata cagtgagcac tgtagtcgcc
     3961 caggctatta aggtgaattg cttttaaaag tcacatttaa caaaggacaa aacacagtat
     4021 actttaacag aactgtcatt taaaatgtgg acaaacttcc taaaatgaac ctatttttaa
     4081 acgtatattc tttgctgtgc atttttggag tgaccaggta ggttcacttg ctaggtccag
     4141 tggcccattc ctgtaaccat tacttggaag atataggcag gaggatcagg agctcagtgc
     4201 atagctaatt caaggtcaac ctatgtaaca taacacttag gctcaaaagc cagaactaaa
     4261 taataataaa gcaaagacag gtgacatgta cctggcaacc tgaggacttg cgtttgatcc
     4321 ctgagatccg agtaagggaa ggaaagaact gacttccaca acttatccac tgacccacac
     4381 acacacacac acctgctccc atacacacac acacacactc accccgtctc tgtcttacac
     4441 acacactcat gttctgattt aacaaacaca ccgacacaca cacacacaca cctgctccca
     4501 tcctctctct cacacacaca caccacagaa taaataaatg taataataac atttttttaa
     4561 ataaagacta gaaggttgaa gcttttgaat ggggaggaca tcactagagt ttggctgaga
     4621 attcagcatt gagtgcaggt caggggggag aacaacaata agtgtccaag gtatgttacc
     4681 acaatttttt tccaaatata aagagaaaat aggaactatg caataggaaa tctagcaaac
     4741 acttatctta actacatgac gagttcacag ctctcttctt gttacctatt actattgtct
     4801 tagtcagggt ttctattcct ggacaaaaca tcatgaccac taagcaaatt ggggaggaaa
     4861 gggtttattc agcttacact ttccacattg ctgttcatca ccaaaggaag tcaggactgg
     4921 aactcaagca ggtcaggagc aggagctgat acagaggcca tggagggatg ttccttactg
     4981 gcttgcctca cctggcttgc tcagcctgct ctcttataga accaagacta ccagcccaga
     5041 gatggtccta cccacaaggg gcctttcccc cttgatcact aattgagaaa atgccttaca
     5101 gttggatctc atggaggcat ttccccaact gaagctcctt tctctgtgat aactccagct
     5161 tgacccaaat tagccagtac aactgtataa caaattattg cataatatat tgacttacaa
     5221 aaaaattctc tctcagggat tgagagggct caggagacca tctcatacaa ttccattcaa
     5281 acgctaacta gaacctgaat catctgagag tttgctatca acttttttct tttttggcat
     5341 tgccagagtt tgaacctagg tacttgagcg tgccaggcaa gctctgtact actgagttta
     5401 tccccaacct ccaataccag tttgtttaac atccaaggtg acttctctac tctggcatct
     5461 aggggctggg atggccagaa caattgaata gtttccaaga ggctgtctta ggctctgaag
     5521 gttgttggac aatctcagtc agactctgta aggatgctgc atttcaactg gacacacttg
     5581 tcagggacat cgctccaaag gaccagggta atagttgcaa ggcttcttga attctagcat
     5641 aagaagttaa acaattatat ttgcaacttt caactaatca gttaccagaa agtcacaagg
     5701 ccaggccaga ttcatggaga tggatctaca tagcgtgaaa actggagatt cagtttactg
     5761 cagaacctgg aacctgactg tggttgatca taacgtagtt gcatcgatta actcctttgg
     5821 tgttctcaag tcccgtccat atccccacta acctacttac cctttcactg ttgctccaag
     5881 gttaccatga gtacatttct tcctcttaat tctttttctc ttctgtgcat tttcaaatgg
     5941 cctatcttgg aattcactca ttctctcttc tgcttgattt actctaacat caatgcttcc
     6001 ctaatgcaat tttattttgt ttagtatacc ttcattatca agaatttcaa ctttttaaaa
     6061 tttgccccca tgtctcagtc aattatatct actaaagtat tgatttattt ctatattttc
     6121 tattggaatt cagtaagttt ccttaaacct ggttcattta tttgctttta attatctgtg
     6181 gaagtttgaa tgagaaatga tccccactga cccaggtatt tgaacattta gtcctcagtt
     6241 ggttgtgatg atcagagaag cttggggaac gcagtcctcc tggagacagc gtatcagtag
     6301 ggcagccttt gctagtatat agcctcacac cacttccagc tcacacttgc tgttttgtgc
     6361 cttcagtggt ggatgtgagc tctcagcttg ctgccccagc atcatacctg ttgctggctt
     6421 ccatgcgtcc ctgccatgat ggagtcttat cactctgaaa acacaggaca aattccttct
     6481 cctataagtc aggctttagt cgtggtattt tatcacaaca atgaaaaagt aactaataca
     6541 gtacacatat gagcatccac ccacactggt tactatcttg acggtttctg gcattttata
     6601 tttatactgt cagatgaggt catagttcct taaacatcct tggttcttat tgatgtgtga
     6661 tgtcattcga ggacaaagga ttaggtattt attccggtct tcataatatg gcttgttttg
     6721 gttttgtttg ttttcagaaa ttgaaaaaag aatgtttttt ttttctctct ctcagcctat
     6781 agtttctaac actatatggc accaaaattc aggttacctg tggcagtgtt aggaggcgat
     6841 ccaagcttag gtttctccta aatcctcagc gggtgagttt ttcaagactc aattagggag
     6901 ctttttataa agggtccttc atctacacaa gcctctgtcc ggggcttgca cagacctcca
     6961 ggtgccttcc aaagccaaca gcctctggtc gtgaactgta gaaagaatca ccagagggac
     7021 ctgccctagt tcctgctcca gaactgtggc tctgagtgtc acaatgctga gcccacagcc
     7081 ggtgctgtgg cagtaaggca aactgagaca acttcctcat ggtgcagagc ataactgtga
     7141 cagatgtcaa ggtcttgagc tccatccgtg aaccctggtc caggatagtt gtgctgctac
     7201 aggttgggaa ccggtggtag caacggtccc tcagagacta gtactaaggc tggccctgag
     7261 ctgcaaggca cagtccttgc tttcatgttg tgccatccag taggtggagt tttgctttcc
     7321 attctaaaag ctgcagttgt cggaaagatt cctaactgta gtactggcca tctatcacag
     7381 gtccagcagc agcatctgca ggtacagggc cacgggatgc atagggttcc acccagaact
     7441 gacatcacgg tgataagttc ttactgcttc aagtctgtgg tcaggattta atcccttctc
     7501 catccccaaa gtggaggtat cattctccat gaagcttgga attgtgggag gggaaatctg
     7561 ggccttggca tcttttcagt gagtcttttc ctgttacagt aaaatcagaa cactatgatg
     7621 tttgtttttt ccaccttact tctttagctc ttttgaagat agtgtggtgc atgaaactct
     7681 gccaggggtg tccaacctta tgacactatg acctgatgct gttatctaca aatgtgttgg
     7741 gcctcacata atctattatg ggatgcatgg taccctaggg ccttaggcta ggctagacac
     7801 accagttagt gcatcagtag ggaggtaggt aaccggcgtg tgatcttacc tagctacctc
     7861 cttaaagagg acaacattta aacacactgg gccaacttta taccacaaag caaagacctc
     7921 caagaaccat gtgctggttg atgtatcaca gtgggaaata caaaacgcta catgtagagg
     7981 tttgatttca aactcaactt cagctttaat caaattctta gacttaactc ccagttcatg
     8041 gcaaatagaa gaaacagaag atgttatgtc tcagcacaga tgcagagaca ataaaataca
     8101 aaattcagga aatgttactg ggaaaaaatg tctcaggaga gaagagacaa atcaaacacg
     8161 tgtgggtcca gagtcttacc agctataaag tttataatac aaagcaagaa actgggccag
     8221 gcatggtggc acacctgcag gctagagatg atcctgagta acctgaggct atggcagggc
     8281 ctgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg
     8341 gggaaaagga agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag
     8401 agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt
     8461 tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg
     8521 tttcagttag cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga
     8581 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
     8641 cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc
     8701 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
     8761 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
     8821 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
     8881 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
     8941 gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag
     9001 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
     9061 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
     9121 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
     9181 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
     9241 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
     9301 ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt
     9361 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
     9421 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
     9481 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat
     9541 ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     9601 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     9661 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
     9721 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     9781 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     9841 ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg
     9901 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9961 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10021 agtgccacct gaataatcgc tagcgaagtt cctatacctt ttgaagaata ggaacttcgg
    10081 aataggaact tcgtcgacaa aggatccttc tcgactagtc gcgctcgcgc gactgacggt
    10141 cgtaagcacc cgcgtacgtg tccaccccgg tcacaacccc ttgtgtcatg tcggcgaccc
    10201 tacgccccca actgagagaa ctcaaaggtt accccagttg gggcactact cccgaaaacc
    10261 gcttctgacc tgggaaaacg tgaagccccg gggcatccgc tgagggttgc cgccggggct
    10321 tcggtgtgtc cgtcagtact agtcgagaaa agatccgata tccttacgga ataccacttg
    10381 ccacctatca ccagatctgg atgcatgatc tcctatttaa atcagctcca gcttttgttc
    10441 cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
    10501 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    10561 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    10621 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    10681 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    10741 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    10801 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    10861 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    10921 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    10981 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    11041 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    11101 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    11161 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    11221 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    11281 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    11341 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    11401 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    11461 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    11521 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    11581 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    11641 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    11701 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    11761 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    11821 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    11881 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    11941 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    12001 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    12061 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    12121 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    12181 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    12241 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    12301 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    12361 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    12421 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    12481 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    12541 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    12601 tccgcgcaca tttccccgaa aagtgc
//
