LOCUS       sequence-82334-e       19587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector for homologous recombination in mouse ES cells; 
            Rosa26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19587)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 19587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: 
            pBT313_pR26-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..19587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1087
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     promoter        1475..1579
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1581..1938
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1581..1772
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1604..1624)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1789..1924
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1973..2767
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1973..2767
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2027..2046)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2637..2656
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2955..2974)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2999..3046
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     RBS             3616..3624
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      8640..8649
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8646..10460
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             10461..10481
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10514..10738
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    10824..15093
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        15142..15635
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     15568..15587
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             15662..16231
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphteria toxin Fragment A"
                     /label=DTA
                     /translation="DDVVILLIFCMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDD
                     DWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELG
                     LSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEIN
                     FETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          16308..16373
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    16447..16671
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(16739..16759)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(16739..16757)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(16778..16794)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16778..16794)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(16791..16813)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    16802..16818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16826..16832,16833..16850,16851..16856))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16871..16892
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(17009..17026)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17180..17768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17260..17279)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17939..18730,18731..18799))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     18562..18581
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18800..18904)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(18930..19385)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(19067..19088)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     19279..19298
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     19512..19534
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     19526..19543
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     19527..19543
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     19553..19572
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        19553..19571
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggascgctt ccggccgacg tctcgtcgct gattggctty ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgn acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttgcccc ctcttcccct cgtgatctgc aactccagtc
     1081 tttctagcct taattaattt aaataggaga tcatgcatcc agatctggtg ataggtggca
     1141 agtggtattc cgtaaggata tcgttttcgg gagtagtgcc ccaactgggg taacctttga
     1201 gttctctcag ttgggggcgt agggtcgccg acgttttcgg gagtagtgcc ccaactgggg
     1261 taacctttga gttctctcag ttgggggcgt agggtcgccg acgttttcgg gagtagtgcc
     1321 ccaactgggg taacctttga gttctctcag ttgggggcgt agggtcgccg acggatcctt
     1381 tgtcgacgaa gttcctattc cgaagttcct attcttcaaa aggtatagga acttcgctag
     1441 cgattattca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1501 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1561 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     1621 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     1681 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     1741 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     1801 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     1861 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     1921 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     1981 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     2041 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     2101 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     2161 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     2221 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     2281 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     2341 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     2401 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     2461 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     2521 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     2581 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     2641 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     2701 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     2761 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     2821 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     2881 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     2941 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     3001 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     3061 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     3121 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     3181 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcaggatc atctctagcc
     3241 tgcaggtgtg ccaccatgcc tggcccagtt tcttgctttg tattataaac tttatagctg
     3301 gtaagactct ggacccacac gtgtttgatt tgtctcttct ctcctgagac attttttccc
     3361 agtaacattt cctgaatttt gtattttatt gtctctgcat ctgtgctgag acataacatc
     3421 ttctgtttct tctatttgcc atgaactggg agttaagtct aagaatttga ttaaagctga
     3481 agttgagttt gaaatcaaac ctctacatgt agcgttttgt atttcccact gtgatacatc
     3541 aaccagcaca tggttcttgg aggtctttgc tttgtggtat aaagttggcc cagtgtgttt
     3601 aaatgttgtc ctctttaagg aggtagctag gtaagatcac acgccggtta cctacctccc
     3661 tactgatgca ctaactggtg tgtctagcct agcctaaggc cctagggtac catgcatccc
     3721 ataatagatt atgtgaggcc caacacattt gtagataaca gcatcaggtc atagtgtcat
     3781 aaggttggac acccctggca gagtttcatg caccacacta tcttcaaaag agctaaagaa
     3841 gtaaggtgga aaaaacaaac atcatagtgt tctgatttta ctgtaacagg aaaagactca
     3901 ctgaaaagat gccaaggccc agatttcccc tcccacaatt ccaagcttca tggagaatga
     3961 tacctccact ttggggatgg agaagggatt aaatcctgac cacagacttg aagcagtaag
     4021 aacttatcac cgtgatgtca gttctgggtg gaaccctatg catcccgtgg ccctgtacct
     4081 gcagatgctg ctgctggacc tgtgatagat ggccagtact acagttagga atctttccga
     4141 caactgcagc ttttagaatg gaaagcaaaa ctccacctac tggatggcac aacatgaaag
     4201 caaggactgt gccttgcagc tcagggccag ccttagtact agtctctgag ggaccgttgc
     4261 taccaccggt tcccaacctg tagcagcaca actatcctgg accagggttc acggatggag
     4321 ctcaagacct tgacatctgt cacagttatg ctctgcacca tgaggaagtt gtctcagttt
     4381 gccttactgc cacagcaccg gctgtgggct cagcattgtg acactcagag ccacagttct
     4441 ggagcaggaa ctagggcagg tccctctggt gattctttct acagttcacg accagaggct
     4501 gttggctttg gaaggcacct ggaggtctgt gcaagccccg gacagaggct tgtgtagatg
     4561 aaggaccctt tataaaaagc tccctaattg agtcttgaaa aactcacccg ctgaggattt
     4621 aggagaaacc taagcttgga tcgcctccta acactgccac aggtaacctg aattttggtg
     4681 ccatatagtg ttagaaacta taggctgaga gagagaaaaa aaaaacattc ttttttcaat
     4741 ttctgaaaac aaacaaaacc aaaacaagcc atattatgaa gaccggaata aatacctaat
     4801 cctttgtcct cgaatgacat cacacatcaa taagaaccaa ggatgtttaa ggaactatga
     4861 cctcatctga cagtataaat ataaaatgcc agaaaccgtc aagatagtaa ccagtgtggg
     4921 tggatgctca tatgtgtact gtattagtta ctttttcatt gttgtgataa aataccacga
     4981 ctaaagcctg acttatagga gaaggaattt gtcctgtgtt ttcagagtga taagactcca
     5041 tcatggcagg gacgcatgga agccagcaac aggtatgatg ctggggcagc aagctgagag
     5101 ctcacatcca ccactgaagg cacaaaacag caagtgtgag ctggaagtgg tgtgaggcta
     5161 tatactagca aaggctgccc tactgatacg ctgtctccag gaggactgcg ttccccaagc
     5221 ttctctgatc atcacaacca actgaggact aaatgttcaa atacctgggt cagtggggat
     5281 catttctcat tcaaacttcc acagataatt aaaagcaaat aaatgaacca ggtttaagga
     5341 aacttactga attccaatag aaaatataga aataaatcaa tactttagta gatataattg
     5401 actgagacat gggggcaaat tttaaaaagt tgaaattctt gataatgaag gtatactaaa
     5461 caaaataaaa ttgcattagg gaagcattga tgttagagta aatcaagcag aagagagaat
     5521 gagtgaattc caagataggc catttgaaaa tgcacagaag agaaaaagaa ttaagaggaa
     5581 gaaatgtact catggtaacc ttggagcaac agtgaaaggg taagtaggtt agtggggata
     5641 tggacgggac ttgagaacac caaaggagtt aatcgatgca actacgttat gatcaaccac
     5701 agtcaggttc caggttctgc agtaaactga atctccagtt ttcacgctat gtagatccat
     5761 ctccatgaat ctggcctggc cttgtgactt tctggtaact gattagttga aagttgcaaa
     5821 tataattgtt taacttctta tgctagaatt caagaagcct tgcaactatt accctggtcc
     5881 tttggagcga tgtccctgac aagtgtgtcc agttgaaatg cagcatcctt acagagtctg
     5941 actgagattg tccaacaacc ttcagagcct aagacagcct cttggaaact attcaattgt
     6001 tctggccatc ccagccccta gatgccagag tagagaagtc accttggatg ttaaacaaac
     6061 tggtattgga ggttggggat aaactcagta gtacagagct tgcctggcac gctcaagtac
     6121 ctaggttcaa actctggcaa tgccaaaaaa gaaaaaagtt gatagcaaac tctcagatga
     6181 ttcaggttct agttagcgtt tgaatggaat tgtatgagat ggtctcctga gccctctcaa
     6241 tccctgagag agaatttttt tgtaagtcaa tatattatgc aataatttgt tatacagttg
     6301 tactggctaa tttgggtcaa gctggagtta tcacagagaa aggagcttca gttggggaaa
     6361 tgcctccatg agatccaact gtaaggcatt ttctcaatta gtgatcaagg gggaaaggcc
     6421 ccttgtgggt aggaccatct ctgggctggt agtcttggtt ctataagaga gcaggctgag
     6481 caagccaggt gaggcaagcc agtaaggaac atccctccat ggcctctgta tcagctcctg
     6541 ctcctgacct gcttgagttc cagtcctgac ttcctttggt gatgaacagc aatgtggaaa
     6601 gtgtaagctg aataaaccct ttcctcccca atttgcttag tggtcatgat gttttgtcca
     6661 ggaatagaaa ccctgactaa gacaatagta ataggtaaca agaagagagc tgtgaactcg
     6721 tcatgtagtt aagataagtg tttgctagat ttcctattgc atagttccta ttttctcttt
     6781 atatttggaa aaaaattgtg gtaacatacc ttggacactt attgttgttc tcccccctga
     6841 cctgcactca atgctgaatt ctcagccaaa ctctagtgat gtcctcccca ttcaaaagct
     6901 tcaaccttct agtctttatt taaaaaaatg ttattattac atttatttat tctgtggtgt
     6961 gtgtgtgtga gagagaggat gggagcaggt gtgtgtgtgt gtgtgtcggt gtgtttgtta
     7021 aatcagaaca tgagtgtgtg tgtaagacag agacggggtg agtgtgtgtg tgtgtgtatg
     7081 ggagcaggtg tgtgtgtgtg tgtgggtcag tggataagtt gtggaagtca gttctttcct
     7141 tcccttactc ggatctcagg gatcaaacgc aagtcctcag gttgccaggt acatgtcacc
     7201 tgtctttgct ttattattat ttagttctgg cttttgagcc taagtgttat gttacatagg
     7261 ttgaccttga attagctatg cactgagctc ctgatcctcc tgcctatatc ttccaagtaa
     7321 tggttacagg aatgggccac tggacctagc aagtgaacct acctggtcac tccaaaaatg
     7381 cacagcaaag aatatacgtt taaaaatagg ttcattttag gaagtttgtc cacattttaa
     7441 atgacagttc tgttaaagta tactgtgttt tgtcctttgt taaatgtgac ttttaaaagc
     7501 aattcacctt aatagcctgg gcgactacag tgctcactgt ataaatgcta gtgtgttttt
     7561 ggtgctgaaa cagaaaggtg gctctagaaa gctggagttc ctcatcttta agttccagac
     7621 tgagatatct agaacttctt caaaaatcca gggaaaggaa agcgatggaa gtgaaaataa
     7681 aaacaagaac atgctttaca tatatttgat tgtgatccct ttggcgggta ctaggaaaac
     7741 cacggatgga attttccttc tagaaagagg tgaggagcag gcagaatgtg aacatctact
     7801 taatgagctg aactggccgg tgccctcaga attgtaaaca ggtacaccac aaatccaggc
     7861 cttggcagac agaccaagtc ttcctctctt cggttttctt tttacagact ggctttcttg
     7921 acaacttcaa gatggagtct catccttgcc ctttttatgg agaggagaag cattgcttct
     7981 agttggtttt agtagaggag tgaagtgcat ttctcagata caaagagagc acttgagacg
     8041 ttcggactca gaatggccaa gcctggcact ttgggaggtc aagaggaggc tggaacggct
     8101 gggagagaaa gcaattagat gttccacccc tttggttttt ctccagacag ggtttctctg
     8161 tgtagccctg actgtcctga agtttgctct gtagaccagg ctggcctcca cccagggatt
     8221 tgcctgcctc tgcctccccg agccccagat ttttattttt atttatatat atattaactt
     8281 ttgaatgaac acaatggaat tgatgagccc ttggagagtg aaacgggatg tcgtgcgtgg
     8341 cagccccggg gatcagctca ctcccacagg cctcacaggc catggagcca agaggcctcc
     8401 ctgcctcggc ctcggcctcg gcctcaacaa aggtggagaa cgcgcaggcc gtccgtccat
     8461 ggggcgggaa gtcgcgcgcc gcggccgctg attggctggc gggcccggtc gcctgatgct
     8521 atttgttgtc cccgcgccaa tgacgcagtc ggcgtccggc gtccacccgc acgtgcagcc
     8581 gtttaagccg cgtcggccgg ccgcgaggag cccggggagc ctggagcgga gagttaaccg
     8641 ccaccatgga tacctacgcc ggagcctacg acagacagag ccgggagaga gagaacagca
     8701 gcgccgccag ccccgccacc cagagaagcg ccaacgagga taaggccgcc gatctgcaga
     8761 gagaggtgga gagggacggc ggcagattca gatttgtggg ccacttcagc gaggcccctg
     8821 gcaccagcgc cttcggcacc gccgagagac ccgagttcga gagaatcctg aacgagtgta
     8881 gggccggcag gctgaacatg atcatcgtgt acgacgtgtc ccggttcagc aggctgaagg
     8941 tgatggacgc catccctatc gtgtccgagc tgctggccct gggcgtgacc atcgtgtcca
     9001 cccaggaagg cgtctttaga cagggcaacg tgatggacct gatccacctg atcatgaggc
     9061 tggacgccag ccacaaggag agcagcctga agagcgccaa gatcctggac accaagaacc
     9121 tgcagaggga gctgggcggc tatgtgggcg gcaaggcccc ctacggcttc gagctggtgt
     9181 ccgagaccaa ggagatcacc cggaacggca ggatggtgaa cgtggtgatc aacaagctgg
     9241 cccacagcac cacccccctg accggcccct tcgagtttga gcccgacgtg atcaggtggt
     9301 ggtggcggga gatcaagacc cacaagcacc tgcctttcaa gcccggcagc caggccgcca
     9361 tccaccccgg cagcatcacc ggcctgtgta agagaatgga cgccgacgcc gtgcccacca
     9421 gaggcgagac catcggcaag aaaaccgcca gcagcgcctg ggaccccgcc accgtgatga
     9481 gaatcctgag ggaccctagg atcgccggct tcgccgccga ggtgatctac aagaagaagc
     9541 ccgacggcac ccccaccacc aagatcgagg gctacagaat ccagagagac cccatcaccc
     9601 tgagacctgt ggagctggac tgtggcccta tcatcgagcc tgccgagtgg tacgagctgc
     9661 aggcctggct ggacggcaga ggcagaggca agggcctgag cagaggccag gccatcctga
     9721 gcgccatgga caagctgtac tgtgagtgtg gcgccgtgat gaccagcaag agaggcgagg
     9781 agagcatcaa ggacagctac cggtgccgga gaagaaaggt ggtggacccc agcgcccctg
     9841 gccagcacga gggcacctgt aatgtgagca tggccgccct ggacaagttc gtggccgagc
     9901 ggatcttcaa caagatccgg cacgccgagg gcgacgagga gaccctggcc ctgctgtggg
     9961 aggccgccag aagattcggc aagctgaccg aggcccccga gaagagcggc gagagggcca
    10021 acctggtggc cgagagagcc gacgccctga acgccctgga ggagctgtac gaggacagag
    10081 ccgccggagc ctatgacggc cctgtgggca ggaagcactt cagaaagcag caggccgccc
    10141 tgaccctgag acagcagggc gccgaggaaa gactggccga gctggaggcc gccgaggccc
    10201 ctaagctgcc cctggatcag tggttccccg aggatgccga cgccgacccc accggcccca
    10261 agtcctggtg gggcagagcc agcgtggacg acaagagggt gttcgtgggc ctgttcgtgg
    10321 ataagatcgt ggtgaccaag agcaccaccg gcaggggcca gggcaccccc atcgagaaga
    10381 gagccagcat cacctgggcc aagcctccca ccgacgacga cgaggatgac gcccaggacg
    10441 gcaccgagga cgtggccgcc cctaagaaaa agcggaaagt gtgatagatc tagagctcgc
    10501 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
    10561 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
    10621 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
    10681 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggac
    10741 gtcctagaga agttcctatt ccgaagttcc tattcttcaa aaggtatagg aacttcgcgg
    10801 ccgcctagta aggggcgcgc cggctagaag atgggcggga gtcttctggg caggcttaaa
    10861 ggctaacctg gtgtgtgggc gttgtcctgc aggggaattg aacaggtgta aaattggagg
    10921 gacaagactt cccacagatt ttcggttttg tcgggaagtt ttttaatagg ggcaaatagg
    10981 aaaatggagg ataggagtca tctggggttt atgcagcaaa actacaggta tattgcttgt
    11041 atccgcctcg gagatttcca tgaggagata aagacatgtc acccgagttt atactctcct
    11101 gcttagatcc tactacagta tgaaatacag tgtygcgagg tagactatgt aagcagattt
    11161 aatcatttta aagagcccag tacttcatat ccatttctcc cgctccttct gcagccttat
    11221 caaaaggtat ttagaacact cattttagcc ccattttcat ttattatact ggcttatcca
    11281 acccctagac agagcattgg cattttccct ttcctgatct tagaagtctg atgactcatg
    11341 aaaccagaca gattagttac atacaccaca aatcgaggct gtagctgggg cctcaacact
    11401 gcagttcttt tataactcct tagtacactt tttgttgatc ctttgccttg atccttaatt
    11461 ttcagtgtct atcacctctc ccgtcaggtg gtgttccaca tttgggccta ttctcagtcc
    11521 agggagtttt acaacaatag atgtattgag aatccaacct aaagcttaac tttccactcc
    11581 catgaatgcc tctctccttt ttctccatta taactgagct atwaccatta atggtttcag
    11641 gtggatgtct cctcccccaa tatacctgat gtatctacat attgccaggc tgatatttta
    11701 agacatwaaa ggtatatttc attattgagc cacatggtat tgattactgc tactaaaatt
    11761 ttgtcattgt acacatctgt aaaaggtggt tccttttgga atgcaaagtt caggtgtttg
    11821 ttgtctttcc tgacctaagg tcttgtgagc ttgtattttt tctatttaag cagtgctttc
    11881 tcttggactg gcttgactca tggcattcta cacgttattg ctggtctaaa tgtgattttg
    11941 ccaagcttct tcaggaccta taattttgct tgacttgtag ccaaacacaa gtaaaatgat
    12001 taagcaacaa atgtatttgt gaagcttggt ttttaggttg ttgtgttgtg tgtgcttgtg
    12061 ctctataata atactatcca ggggctggag aggtggctcg gagttcaaga gcacagactg
    12121 ctcttccaga agtcctgagt tcaattccca gcaaccacat ggtggctcac aaccatctgt
    12181 aatgggatct gatgccctct tctggtgtgt ctgaagacca caagtgtatt cacattaaat
    12241 aaataatcct ccttcttctt cttttttttt ttttaaagag aatwctgtct ccagtagaat
    12301 tactgaagta atgaaatact ttgtgtttgt tccaatatgg wagccaataa tcaaatactc
    12361 ttwagcactg gaaatgtacc aaggaactat tttatttaag tgwactgtgg acagaggagc
    12421 cataactgca gacttgtggg atacagaaga ccaatgcaga cttaatgtct tttctcttac
    12481 actaagcaat aaagaaataa aaattgaact tctagtatcc tatttgttaa actgctagct
    12541 ttactaactt ttgtgcttca tctatacaaa gctgaaagct aagtctgcag ccattactaa
    12601 acatgaaagc aagtaatgat aattttggat ttcaaaaatg tagggccaga gtttagccag
    12661 ccagtggtgg tgcttgcctt tatgccttaa tcccagcact ctggaggcag agacaggcag
    12721 atctctgagt ttgagcccag cctggtctac acatcaagtt ctatctagga tagccaggaa
    12781 tacacacaga aaccctgttg gggagggggg ctctgagatt tcataaaatt ataattgaag
    12841 cattccctaa tgagccacta tggatgtggc taaatccgtc tacctttctg atgagatttg
    12901 ggtattattt tttctgtctc tgctgttggt tgggtctttt gacactgtgg gctttcttaa
    12961 agcctccttc cctgccatgt ggtctcttgt ttgctactaa cttcccatgg cttaaatggc
    13021 atggcttttt gccttctaag ggcagctgct gagwtttgca gcctgatttc cagggtgggg
    13081 ttgggaaatc tttcaaacac taaaattgtc ctttaatttt tttttaaaaa atgggttata
    13141 taataaacct cataaaatag ttatgaggag tgaggtggac taatattaat gagtccctcc
    13201 cctataaaag agctattaag gctttttgtc ttatactaac ttttttttta aatgtggtat
    13261 ctttagaacc aagggtctta gagttttagt atacagaaac tgttgcatcg cttaatcaga
    13321 ttttctagtt tcaaatccag agaatccaaa ttcttcacag ccaaagtcaa attaagaatt
    13381 tctgacttta atgttatttg ctactgtgaa tataaaatga tagcttttcc tgaggcaggg
    13441 tctcactatg tatctctgcc tgatctgcaa caagatatgt agactaaagt tctgcctgct
    13501 tttgtctcct gaatactaag gttaaaatgt agtaatactt ttggaacttg caggtcagat
    13561 tcttttatag gggacacact aagggagctt gggtgatagt tggtaaatgt gtttaagtga
    13621 tgaaaacttg aattattatc accgcaacct actttttaaa aaaaaaagcc aggcctgtta
    13681 gagcatgcta agggatccct aggacttgct gagcacacaa gagtagtact tggcaggctc
    13741 ctggtgagag catatttcaa aaaacaaggc agacaaccaa gaaactacag taaggttacc
    13801 tgtctttaac catctgcata tacacaggga tattaaaata ttccaaataa tatttcattc
    13861 aagttttccc ccatcaaatt gggacatgga tttctccggt gaataggcag agttggaaac
    13921 taaacaaatg ttggttttgt gatttgtgaa attgttttca agtgatagtt aaagcccatg
    13981 agatacagaa caaagctgct atttcgaggt ctcttggtta tactcagaag cacttctttg
    14041 ggtttccctg cactatcctg atcatgtgct aggcctwcct taggctgatt gttgttcaaa
    14101 taacttaagt ttcctgtcag gtgatgtcat atgatttcat atatcaaggc aaaacatgtt
    14161 atatatgtta aacatttgka cttaatgtga aagttaggtc tttgtgggtt ttgattttaa
    14221 tttcaaaacc tgagctaaat aagtcatttt acatgtctta catttggtga attgtatatt
    14281 gtggtttgca ggcaagactc tctgacctag taaccctcct atagagcact ttgctgggtc
    14341 acaagtctag gagtcaagca tttcaccttg aagttgagac gttttgttag tgtatactag
    14401 ttatatgttg gaggacatgt ttatccagaa gatattcagg actatttttg actgggctaa
    14461 ggaattgatt ctgattagca ctgttagtga gcattgagtg gcctttaggc ttgaattgga
    14521 gtcacttgta tatctcaaat aatgctggcc ttttttwaaa agcccttgtt ctttatcacc
    14581 ctgttttcta cataattttt gttcaaagaa atacttgttt ggatctcctt ttgacaacaa
    14641 tagcatgttt tcaagccata ttttttttcc tttttttttt tttttttggt ttttcgagac
    14701 agggtttctc tgtatagccc tggctgtcct ggaactcact ttgtagacca ggctggcctc
    14761 gaactcagaa atccgcctgc ctctgcctcc tgagtgccgg gattaaaggc gtgcaccacc
    14821 acgcctggct aagttggata ttttgtatat aactataacc aatactaact ccactgggtg
    14881 gatttttaat tcagtcagta gtcttaagtg gtctttattg gcccttatta aaatctactg
    14941 ttcactctaa cagaggctgt tggactagtg gsactaagca acttcctacg gatatactag
    15001 cagataaggg tcagggatag aaactagtct agcgttttgt atacctacca gcttatacta
    15061 ccttgttctg atagaaatat ttaggacatc tagcttatcg atccgtcgac ggtatcgata
    15121 agcttgatat cgaattctac cgggtagggg aggcgctttt ccaaggcagt ctgagcatgc
    15181 gcttagcagc cccgctggca cttggcgcta cacaagtggc ctytggcctc gcacacattc
    15241 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
    15301 twctcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt saggacgtga
    15361 caaatggaag tagcacgtct cactagtctc gtcagatgga cagcaccgct gagcaatgga
    15421 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
    15481 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
    15541 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
    15601 cgctgttctc ctcttcctca tctccgggcc tttcgacctg caggtcctcg ccatggatcc
    15661 tgatgatgtt gttattcttc taatcttttg tatggaaaac ttttcttcgt accacgggac
    15721 taaacctggt tatgtagatt ccattcaaaa aggtatacaa aagccaaaat ctggtacaca
    15781 aggaaattat gacgatgatt ggaaagggtt ttatagtacc gacaataaat acgacgctgc
    15841 gggatactct gtagataatg aaaacccgct ctctggaaaa gctggaggcg tggtcaaagt
    15901 gacgtatcca ggactgacga aggttctcgc actaaaagtg gataatgccg aaactattaa
    15961 gaaagagtta ggtttaagtc tcactgaacc gttgatggag caagtcggaa cggaagagtt
    16021 tatcaaaagg ttcggtgatg gtgcttcgcg tgtagtgctc agccttccct tcgctgaggg
    16081 gagttctagc gttgaatata ttaataactg ggaacaggcg aaagcgttaa gcgtagaact
    16141 tgagattaat tttgaaaccc gtggaaaacg tggccaagat gcgatgtatg agtatatggc
    16201 tcaagcctgt gcaggaaatc gtgtcaggcg atctctttgt gaaggaacct tacttctgtg
    16261 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
    16321 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
    16381 cctatggaac tgatgaatgg gagcagtggt ggaatgcaga tcctagagct cgctgatcag
    16441 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
    16501 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
    16561 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
    16621 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
    16681 cggaaagaac cagctggggc tcgacctcga gggggggccc ggtacccagc ttttgttccc
    16741 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    16801 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    16861 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    16921 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    16981 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    17041 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    17101 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    17161 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    17221 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    17281 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    17341 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    17401 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    17461 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    17521 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    17581 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    17641 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    17701 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    17761 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    17821 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    17881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    17941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    18001 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    18061 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    18121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    18181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    18241 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    18301 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    18361 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    18421 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    18481 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    18541 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    18601 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    18661 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    18721 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    18781 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    18841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    18901 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
    18961 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
    19021 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
    19081 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
    19141 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
    19201 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
    19261 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
    19321 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
    19381 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    19441 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    19501 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
    19561 ctcactatag ggcgaattgg agctcca
//
