LOCUS       sequence-82341-e         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV plasmid with CAGGS promoter driving expression of EmGFP. 
            Contains WPRE..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS2058
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(43..62)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          45..177
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(319..341)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcggccgc ctgggagtgg acacctgtgg agagaaaggc
       61 aaagtggatg tcagtaagac caataggtgc ctatcagaaa cgcaagagtc ttctctgtct
      121 cgacaagccc agtttctatt ggtctcctta aacctgtctt gtaaccttga tacttacctg
      181 cccagtgcct cacgaccaac ttcggcgcgc caattctttg ccaaaatgat gagacagcac
      241 aacaaccagc acgttgccca ggagctgtag gaaaaagaag aaggcatgaa catggttagc
      301 agaggctcta gagccgccgg tcacacgcca gaagccgaac cccgccctgc cccgtccccc
      361 ccgaaggcag ccgtccccct gcggcagccc cgaggctgga gatggagaag gggacggcgg
      421 cgcggcgacg cacgaaggcc ctccccgccc atttccttcc tgccggcgcc gcaccgcttc
      481 gcccgcgccc gctagagggg gtgcggcggc gcctcccaga tttcggctcc gccagatttg
      541 ggacaaagga agtccctgcg ccctctcgca cgattaccat aaaaggcaat ggctgcggct
      601 cgccgcgcct cgacagccgc cggcgctccg gggccgccgc gcc
//
