LOCUS       sequence-82342-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV plasmid with CAGGS promoter driving expression of EmGFP. 
            Contains WPRE..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS2058
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(71..91)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(489..500)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    621..742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(632..651)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     686..705
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agtaagcggc cgcgatcgct caacctctgg attacaaaat ttgtgaaaga ttgactggta
       61 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
      121 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
      181 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
      241 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
      301 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
      361 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
      421 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
      481 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
      541 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
      601 ccccgcgatc gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      661 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      721 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
      781 gggggagatg tggga
//
