LOCUS       sequence-82343-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived 
            from the human PCP2 gene and designed for expression in ON Bipolar 
            cells of the retina..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Korecki AJ, Cueva-Vargas JL, Fornes O, Agostinone J, Farkas RA, 
            Hickmott JW, Lam SL, Mathelier A, Zhou M, Wasserman WW, Di Polo A, 
            Simpson EM
  TITLE     Human MiniPromoters for ocular-rAAV expression in ON bipolar, cone, 
            corneal, endothelial, Muller glial, and PAX6 cells.
  JOURNAL   Gene Ther. 2021 Feb 2. pii: 10.1038/s41434-021-00227-z. doi: 
            10.1038/s41434-021-00227-z.
  PUBMED    33531684
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene Ther. 
            2021 Feb 2. pii: 10.1038/s41434-021-00227-z. doi: 
            10.1038/s41434-021-00227-z."
COMMENT     Sequence Label: pEMS2115
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(36..57)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(297..316)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     644..665
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(776..796)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
       61 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      121 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      181 ccttcaccta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
      241 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      301 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      361 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      421 acaactacaa cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
      481 tgaacttcaa gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      541 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
      601 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      661 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gcggccgcga
      721 tcgctcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
      781 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
      841 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatga
//
