LOCUS       sequence-82349-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of sgRNA precursor with CspCI placeholder for target 
            cloning (see comments)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ283
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(27..78)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     promoter        complement(197..553)
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     primer_bind     complement(590..606)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(590..606)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(603..625)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    614..630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(638..644,645..662,663..668))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    683..704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(821..838)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgactctaga ggatccccac ggtacccctg tttcgtcctc acggactcat cagaccggaa
       61 agcacatccg gtgacagggc accaccgact cggtgccact cacttttttc aagttgataa
      121 cggactagcc ttattttaac ttgctatttc tagctctaaa acagagagat aacccacaag
      181 aattgagtta agcccattct tcggtacagg ttatgttttt tggcaacagc aactattcaa
      241 atcaactgaa agttttttag aattcaacaa cttccaatac tcaagtgtcc aagctagcaa
      301 actgtttaat aatcgtgacc caacattcct ttcatgcgca ccctcagcgt cacataaatc
      361 aacactttac aacacctatt caaagcttct atatgccttc gtcatttaaa tgtctaacat
      421 ttcaaaaaag tgtttgctaa tggtaaattc ttgagttttc ttggaagcac atatataacg
      481 agtgtgactg ttctacgctc cttgctgact agtcatcagt ctacgcatgc caactaccac
      541 caacataagc aaaaggtacc cggggtaccg agctcgaatt cgtaatcatg tcatagctgt
      601 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      661 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      721 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      781 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
      841 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggt
//
