LOCUS       sequence-82355-e         597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Artificial transcription factor with no activator domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Senecal A, Munsky B, Proux F, Ly N, Braye FE, Zimmer C, Mueller F, 
            Darzacq X
  TITLE     Transcription Factors Modulate c-Fos Transcriptional Bursts.
  JOURNAL   Cell Rep. 2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053.
  PUBMED    24981864
REFERENCE   2  (bases 1 to 597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053."
COMMENT     Sequence Label: pAF1
FEATURES             Location/Qualifiers
     source          1..597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttgcca ccatggaccc
      121 cattcgtccg cgcaggccaa gtcctgcccg cgagcttctg cctggacccc aaccggatag
      181 ggttcagccg actgcagatc gtggggtgtc tgcgcctgct ggcagccctc tggatggctt
      241 gcccgctcgg cggacggtgt cccggacccg gctgccatct ccccctgcgc cctcacctgc
      301 gttctcggcg ggcagcttca gcgatctgct ccgtccgttc gatccgtcgc ttcttgatac
      361 atcgcttctt gattcgatgc ctgccgtcgg cacgccgcat acagcggctg ccccagcaga
      421 gtgggatgag gcgcaatcgg ctctgcgtgc agccgatgac ccgccaccca ccgtgcgtgt
      481 cgctgtcact gccgcgcggc cgccgcgcgc caagccggcc ccgcgacggc gtgctgcgca
      541 accctccgac gcttcgccgg ccgcgcaggt ggatctacgc acgctcggct acagtca
//
