LOCUS       sequence-82357-e         521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Artificial transcription factor with VP16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Senecal A, Munsky B, Proux F, Ly N, Braye FE, Zimmer C, Mueller F, 
            Darzacq X
  TITLE     Transcription Factors Modulate c-Fos Transcriptional Bursts.
  JOURNAL   Cell Rep. 2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053.
  PUBMED    24981864
REFERENCE   2  (bases 1 to 521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2014 Jun 25. pii: S2211-1247(14)00447-1. doi: 
            10.1016/j.celrep.2014.05.053."
COMMENT     Sequence Label: pAF1-VP16
FEATURES             Location/Qualifiers
     source          1..521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttgccacca tggaccccat
      121 tcgtccgcgc aggccaagtc ctgcccgcga gcttctgccc ggaccccaac cggatagggt
      181 tcagccgact gcagatcgtg gggtgtctgc gcctgctggc agccctctgg atggcttgcc
      241 cgctcggcgg acggtgtccc ggacccggct gccatctccc cctgcgccct cacctgcgtt
      301 ctcggcgggc agcttcagcg atctgctccg tccgttcgat ccgtcgcttc ttgatacatc
      361 gcttcttgat tcgatgcctg ccgtcggcac gccgcataca gcggctgccc cagcagagtg
      421 ggatgaggcg caatcggctc tgcgtgcagc cgatgacccg ccacccaccg tgcgtgtcgc
      481 tgtcactgcc gcgcggccgc cgcgcgccaa gccggccccg c
//
