LOCUS       sequence-82358-e        1098 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Panagrolaimus superbus DJ-1. Potent antioxidant and 
            molecular chaperone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1098)
  TITLE     PsuDJ1.1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PsuDJ-1.1
FEATURES             Location/Qualifiers
     source          1..1098
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     73..92
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        73..91
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    92..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             131..153
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             164..181
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             191..208
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             215..259
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             275..289
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             924..941
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(994..1012)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1008..1055
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atcgatctcg
       61 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
      121 tagaaataat tttgtttaac tttaagaagg agatatacat atgcaccatc atcatcatca
      181 ttcttctggt ctggtgccac gcggttctgg tatgaaagaa accgctgctg ctaaattcga
      241 acgccagcac atggacagcc cagatctggg taccgatgac gacgacaaga tgacgacaaa
      301 aattttggtt attggatttc cagaaactga agaaacagaa ttaattataa ctgttgatat
      361 tcttcgccgt gctgaattaa atgtaacaat cgctaatttg aatgacgaag aatattttac
      421 ttgtgttcaa aaaaccacaa ttaaagctga caaattattt aaagatgttg aaaatgaaac
      481 ttttgatgct gtaattattc caggcggccc gggatcttat aaagttgcga ataatgatcg
      541 ccttgtaaca ttccttaaaa agcatgatga agctggaaaa cttttagctg caatttgtgg
      601 agcgccagtt atttttgcac aaaataaaat tggtgaaggt ggaaaaatga catcatatcc
      661 aaatgataaa gaaaaaattg aaaaagctgg ttttgtttat gatgaattgg atgttgttgt
      721 ttcaaataat attgtaacga gtcgagctcc aggaacagct tttgaatttg ctttaaaact
      781 tgttgaaatt ttagtgggtg aaacgacttc tatggatctt gccaaaacac ttctatatgt
      841 tcaataaacc gggcttctcc tcaaccatgg cgatatcgga tccgaattcg agctccgtcg
      901 acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca
      961 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     1021 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
     1081 tggcgaatgg gacgcgcc
//
