LOCUS       sequence-82359-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of humanized Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ222
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(90..101)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cttccgttgt gtgatcagtt tggcgttcag cagctgccgc caataatttt tcattttctt
       61 gacaacttct tctgagggga cgttatcact cttccctcta tttttatcgg atcttgtcaa
      121 cactttatta tcaatagaat catctttgag aaaagactgg ggcacgatat gatccacgtc
      181 gtagtcggag agccgattga tgtccagttc ctgatccacg tacatgtccc tgccgttctg
      241 caggtagtac aggtagagct tctcattctg aagctgggtg ttttcaactg ggtgttcctt
      301 aaggatttgg gaccccagtt cttttatacc ctcttcaatc ctcttcatcc tttccctact
      361 gttcttctgt cccttctggg tagtttggtt ctctcgggcc atctcgataa cgatattctc
      421 gggcttatgc cttcccatta ctttgacgag ttcatccacg accttaacgg tctgcagtat
      481 tccctttttg atagctgggc tacctgcaag attagcgatg tgctcgtgaa gactgtcccc
      541 ctggccagaa acttgtgctt tctggatgtc ctccttaaag gtgagagagt catcatggat
      601 caactgcatg aagttccggt tggcaaatcc atcggactta agaaaatcca ggattgtctt
      661 tccagactgc ttatctcgga tcccattgat cagttttctt gacagccgcc cccatcctgt
      721 atatcggcgc ctcttgagct gtttcatgac tttgtcgtcg aagagatgag cgtaagtttt
      781 caagcgttct tcaatcatct ccctatcttc aaacaacgta agggtgagga caatgt
//
