LOCUS       sequence-82361-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human SRP14 and SRP9 proteins as a fusion protein in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  TITLE     Strub Lab 14-9VN plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p14-9VN
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..66
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             338..346
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      793..802
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(844..865)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagaat tgatctacca tggactacaa agacgatgac
       61 gacaagcttg cggccgcgaa ttctatggtg ttgttggaga gcgagcagtt cctgacggag
      121 ctgaccagac ttttccagaa gtgtcggacg tcgggcagcg tctatatcac cttgaagaag
      181 tatgacggtc gaaccaaacc cattccaaag aaaggtactg tggagggctt tgagcccgca
      241 gacaacaagt gtctgttaag agctaccgat gggaagaaga agatcagcac tgtggtgagc
      301 tccaaggaag tgaataagtt tcagatggct tattcaaacc tccttagagc taacatggat
      361 gggttgaaga agagagacaa aaagaacaaa actaagaaga ccaaagcagc agcaacagca
      421 gcagcagcag cacctgccgc agcagcaaca gcagcaacaa cagcagcaac aacagcagca
      481 acagcagcac agtcccccgg gggaatgccg cagtaccaga cctgggagga gttcagccgc
      541 gctgccgaga agctttacct cgctgaccct atgaaggcac gtgtggttct caaatatagg
      601 cattctgatg ggaacttgtg tgttaaagca acagatgatt tagtttgttt ggtgtataaa
      661 acagaccaag ctcaagatgt aaagaagatt gagaaattcc acagtcaact aatgcgactt
      721 atggtagcca aggaagcccg caatgttacc atggaaactg agggggtacc agtcgactct
      781 agaagatcca tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      841 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      901 ga
//
