LOCUS       sequence-82363-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of humanized Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ222
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(206..611)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 ccttcttgga ggcttccagc gcctcatctg gaagtggaac acctgtagca tcgatagcag
       61 caccaccaat taaatgattt tcgaaatcga acttgacatt ggaacgaaca tcagaaatag
      121 ctttaagaac cttaatggct tcggctgtga tttcttgacc aacgtggtca cctggcaaaa
      181 cgacgatctt cttaggggca gacattagaa tggtatatcc ttgaaatata tatatatatt
      241 gctgaaatgt aaaaggtaag aaaagttaga aagtaagacg attgctaacc acctattgga
      301 aaaaacaata ggtccttaaa taatattgtc aacttcaagt attgtgatgc aagcatttag
      361 tcatgaacgc ttctctattc tatatgaaaa gccggttccg gcgctctcac ctttcctttt
      421 tctcccaatt tttcagttga aaaaggtata tgcgtcaggc gacctctgaa attaacaaaa
      481 aatttccagt catcgaattt gattctgtgc gatagcgccc ctgtgtgttc tcgttatgtt
      541 gaggaaaaaa ataatggttg ctaagagatt cgaactcttg catcttacga tacctgagta
      601 ttcccacagt taactgcggt caagatattt cttgaatcag gcgccttaga ccgctcggcc
      661 aaacaaccaa ttacttgttg agaaatagag tataattatc ctataaatat aacgtttttg
      721 aacacacatg aacaaggaag tacaggacaa ttgattttga agagaatgtg gattttgatg
      781 taattgttgg gattccattt ttaataaggc aataatatta ggtatgtaga tat
//
