LOCUS       sequence-82371-e        5836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  baculovirus IE-1 promoter driving firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Anderson MA, Gross TL, Myles KM, Adelman ZN
  TITLE     Validation of novel promoter sequences derived from two endogenous 
            ubiquitin genes in transgenic Aedes aegypti.
  JOURNAL   Insect Mol Biol. 2010 Aug;19(4):441-9. doi: 
            10.1111/j.1365-2583.2010.01005.x. Epub 2010 Apr 26.
  PUBMED    20456509
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2583.2010.01005.x"; journalName: "Insect Mol Biol"; 
            date: "2010-08"; volume: "19"; issue: "4"; pages: "441-9"
COMMENT     Sequence Label: pGL3-IE1
FEATURES             Location/Qualifiers
     source          1..5836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        22..504
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        508..1099
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             1106..2758
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1172..1190)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2799..2920
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2810..2829)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2864..2883
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3168..3185)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3339..3927)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3419..3438)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4098..4889,4890..4958))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4721..4740
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4959..5063)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5090..5545
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5177..5196)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5387..5408
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5676..5724
                     /note="synthetic polyadenylation signal"
     misc_feature    5738..5829
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5778..5797
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgc ccgcgtaaaa cacaatcaag tatgagtcat aagctgatgt
       61 catgttttgc acacggctca taaccgaact ggctttacga gtagaattct acttgtaacg
      121 cacgatcagt ggatgatgtc atttgttttt caaatcgaga tgatgtcatg ttttgcacac
      181 ggctcataaa ctcgctttac gagtagaatt ctacgtgtaa cgcacgatcg attgatgagt
      241 catttgtttt gcaatatgat atcatacaat atgactcatt tgtttttcaa aaccgaactt
      301 gatttacggg tagaattcta cttgtaaagc acaatcaaaa agatgatgtc atttgttttt
      361 caaaactgaa ctcgctttac gagtagaatt ctacgtgtaa aacacaatca agaaatgatg
      421 tcatttgtta taaaaataaa agctgatgtc atgttttgca catggctcat aactaaactc
      481 gctttacggg tagaattcta cgcgcgtcga tgtctttgtg atgcgcgcga catttttgta
      541 ggttattgat aaaatgaacg gatacgttgc ccgacattat cattaaatcc ttggcgtaga
      601 atttgtcggg tccattgtcc gtgtgcgcta gcatgcccgt aacggacctc gtacttttgg
      661 cttcaaaggt tttgcgcaca gacaaaatgt gccacacttg cagctctgca tgtgtgcgcg
      721 ttaccacaaa tcccaacggc gcagtgtact tgttgtatgc aaataaatct cgataaaggc
      781 gcggcgcgcg aatgcagctg atcacgtacg ctcctcgtgt tccgttcaag gacggtgtta
      841 tcgacctcag attaatgttt atcggccgac tgttttcgta tccgctcacc aaacgcgttt
      901 ttgcattaac attgtatgtc ggcggatgtt ctatatctaa tttgaataaa taaacgataa
      961 ccgcgttggt tttagagggc ataataaaag aaatattgtt atcgtgttcg ccattagggc
     1021 agtataaatt gacgttcatg ttggatattg tttcagttgc aagttgacac tggcggcgac
     1081 aagatcgtga acaaccaagt gacccatgga agacgccaaa aacataaaga aaggcccggc
     1141 gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag
     1201 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac
     1261 ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct
     1321 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt
     1381 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg
     1441 tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg
     1501 gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat
     1561 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct
     1621 acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat
     1681 tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca
     1741 tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat
     1801 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac
     1861 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct
     1921 gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt
     1981 ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat
     2041 tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca
     2101 tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac
     2161 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa
     2221 ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt
     2281 gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat
     2341 tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt
     2401 cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc
     2461 tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct
     2521 tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac
     2581 gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt
     2641 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc
     2701 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaatt
     2761 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg
     2821 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     2881 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     2941 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     3001 caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag
     3061 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     3121 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac
     3181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4021 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4081 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4141 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4201 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4261 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4321 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4381 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4441 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4501 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4561 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4621 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4681 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4741 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4801 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4861 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4921 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4981 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5041 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     5101 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5161 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5221 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5281 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5341 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5401 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5461 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5521 aacaaaatat taacgcttac aatttgccat tcgccattca ggctgcgcaa ctgttgggaa
     5581 gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt
     5641 aatattaagg tacgggaggt acttggagcg gccgcaataa aatatcttta ttttcattac
     5701 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
     5761 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
     5821 catttctcta tcgata
//
