LOCUS       sequence-82373-e        5935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mos1 transformation vector containing MCS, loxP, attP, 2 x 5HE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Anderson MA, Gross TL, Myles KM, Adelman ZN
  TITLE     Validation of novel promoter sequences derived from two endogenous 
            ubiquitin genes in transgenic Aedes aegypti.
  JOURNAL   Insect Mol Biol. 2010 Aug;19(4):441-9. doi: 
            10.1111/j.1365-2583.2010.01005.x. Epub 2010 Apr 26.
  PUBMED    20456509
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2583.2010.01005.x"; journalName: "Insect Mol Biol"; 
            date: "2010-08"; volume: "19"; issue: "4"; pages: "441-9"
COMMENT     Sequence Label: pMos-3xP3DsRed-attP
FEATURES             Location/Qualifiers
     source          1..5935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..19)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        87..191
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(192..260,261..1052)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(410..429)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1223..1811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1712..1731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1965..1982
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2099..2120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2135..2140,2141..2158,2159..2165)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2173..2189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2178..2200
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2197..2213
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2197..2213
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2234..2252
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      3699..3708
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3705..3707,3708..3710,3711..4385)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(3888..3908)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4106..4125
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4300..4323
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    4506..4627
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4543..4562)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4597..4616
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(4646..4679)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(4961..4994)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     5532..5553
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5757..5776)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5876..5898
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
       61 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      121 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      181 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      241 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      301 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      361 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      421 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      481 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      541 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      601 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      661 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      721 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      781 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      841 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      901 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      961 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1021 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1081 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1141 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1201 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1261 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1321 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1381 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1441 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1501 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1561 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1621 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1681 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1741 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1801 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1861 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1921 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1981 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2041 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2101 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2161 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2221 acgccaagct cggaattaac cctcactaaa gggaacaaaa gctttatggt ttatgattat
     2281 caatttcgtt cccattgctg gaatatcttt cgggttgttt gttatgcctc gcttttcact
     2341 gcgtgctagc agttcccttt tccttaggaa tgaccatgcg cacttccact ttcgtgggat
     2401 aaaaatggta aatccggctt tttcttaaag ctaccaaatg atgtagatag gcatcccaca
     2461 gtacgggctc cagtcggtga atatagaaac tatcaggtgt acaagtatga aatgtcgttt
     2521 tttttaaatc aaaaaacacg ttaaattttg tggaaaaaga aaaaatcatt tattcaaagt
     2581 atttgccgtc gctagctaca catttttccc atctctcggg caatttgtgg attccacgcc
     2641 agtagaactc atcgtctttt gcggcgaacc attcatcgag ccattttttc acactttcgt
     2701 aagaatcgaa gcgctgctca gcgagtgcgt gtcccatcga agcgaatagg tggtaatcgg
     2761 atggggccag gtctggtgag taagccgcat gcggaagcac ttcccaattg agtgtttcca
     2821 acgtgtcgcg aaccgctctt gccgtatgtg atggagcgtt gtcatggaga aaaatgaccc
     2881 tgtgttgtct tttttgatat tccggtcgtt ttctctgaag cgcacggttc aaattgatca
     2941 attgttgttg gtagcgtgcc gtattcaccg tttcgccggg tttcaagagc tcatagtaaa
     3001 tgacaccgct ctgatcccac caaacacaga gcatcgtctt cttgccaaag cgattcggtc
     3061 gagcagtcga tgtggccggt tgtccaggat caacgtatga ctttttacgt ttaggattaa
     3121 caaaaaagat ccatttttca tctccagtaa cgatacgatg caaaaacgac ttccttttgt
     3181 atcgtgaaag caaaatttcg catgtgtttt tgcgcctctc catctgcctc tcgttcaact
     3241 catgtggcac ccatctaccg accttctgaa tctttcccat ctctcgcaag cgattggaaa
     3301 ctgcttgttg acttacttcc aactgctctg cgagttgttt ttgcgtttga gcatcgtctt
     3361 catccaataa tgcttgcagt tcggcgtctt cgtacctttt tggcggtttt ccgtgctctt
     3421 tgtcgtcgaa attcgagctc gcccggggat ctaattcaat tagagactaa ttcaattaga
     3481 gctaattcaa ttaggatcca agcttatcga tttcgaaccc tcgaccgccg gagtataaat
     3541 agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct
     3601 aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc ggggtaccgc
     3661 tagagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg cgctcctcca
     3721 agaacgtcat caaggagttc atgcgcttca aggtgcgcat ggagggcacc gtgaacggcc
     3781 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggccac aacaccgtga
     3841 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
     3901 tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac tacaagaagc
     3961 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     4021 tgaccgtgac ccaggactcc tccctgcagg acggctgctt catctacaag gtgaagttca
     4081 tcggcgtgaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     4141 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc cacaaggccc
     4201 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac atggccaaga
     4261 agcccgtgca gctgcccggc tactactacg tggactccaa gctggacatc acctcccaca
     4321 acgaggacta caccatcgtg gagcagtacg agcgcaccga gggccgccac cacctgttcc
     4381 tgtagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
     4441 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     4501 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4561 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4621 tatcttaaag cttatcgata cgcgtataac ttcgtatagc atacattata cgaagttata
     4681 tcgtagcagc tcacggttag ggataacagg gtaattattt gaggaggttt ctctgtaaat
     4741 aatgactctc ttaaggtagc ctagaacaaa acgtcgtgag acagtttggt ttaattaacg
     4801 cgtacggcgc gccactagtg atatctactc gagtgaattc ttatttgagg aggtttctct
     4861 gtaaataatg atcgtagcag ctcacggtta caaaacgtcg tgagacagtt tggtagggat
     4921 aacagggtaa tgactctctt aaggtagcct agaaagatct ataacttcgt atagcataca
     4981 ttatacgaag ttatgtagtg ccccaactgg ggtaaccttt gagttctctc agttgggggc
     5041 gtagcctagg ccggccgatc tcgtcgacgt caaaatcacc acttttgaag cgttgaaacc
     5101 accgttcaca cgttttcaca gttggtactt gttcgccaaa ggcttcaaca agcattcggt
     5161 gcgattccgc agctgttttc ttcaaatgaa aacagaaaat taatactgtc cgcgtttgct
     5221 ctttattcgg cacgaaactc gacatgttga ctgcactgag agtaaacaat tatgacgctc
     5281 aattcgcgcc aaactatggt ggttcgacag tcaaggttga cacttcacaa ggtcaaagtt
     5341 ttatgacaat cgataaatat ttacgtttgc gagacatcta tatgttcgaa ccgacattcc
     5401 ctacttgtac acctggtaaa aaattaaaac taggtccccg ttcttacgca tatatacaca
     5461 ggtacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5521 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     5581 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     5641 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt cctgatgcgg
     5701 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     5761 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     5821 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     5881 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagg
//
