LOCUS       sequence-82374-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human SRP14A5 and SRP9 proteins as a fusion protein in 
            mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  TITLE     Strub Lab 14-9VN plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p14-9VNA5
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..64
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             336..344
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      791..800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(842..863)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cagagctcgt ttagtgaacc gtcagaattg atctaccatg gactacaaag acgatgacga
       61 caagcttgcg gccgcgaatt ctatggtgtt gttggagagc gagcagttcc tgacggagct
      121 gaccagactt ttccagaagt gtcggacgtc gggcagcgtc tatatcacct tgaagaagta
      181 tgacggtcga accaaaccca ttccaaagaa aggtactgtg gagggctttg agcccgcaga
      241 caacaagtgt ctgttaagag ctaccgatgg gaagaagaag atcagcactg tggtgagctc
      301 caaggaagtg aataagtttc agatggctta ttcaaacctc cttagagcta acatggatgg
      361 gttgaaggca gcagcagcag caaacaaaac taagaagacc aaagcagcag caacagcagc
      421 agcagcagca cctgccgcag cagcaacagc agcaacaaca gcagcaacaa cagcagcaac
      481 agcagcacag tcccccgggg gaatgccgca gtaccagacc tgggaggagt tcagccgcgc
      541 tgccgagaag ctttacctcg ctgaccctat gaaggcacgt gtggttctca aatataggca
      601 ttctgatggg aacttgtgtg ttaaagcaac agatgattta gtttgtttgg tgtataaaac
      661 agaccaagct caagatgtaa agaagattga gaaattccac agtcaactaa tgcgacttat
      721 ggtagccaag gaagcccgca atgttaccat ggaaactgag ggggtaccag tcgactctag
      781 aagatccatc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      841 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      901 gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc
      961 cgtgccc
//
