LOCUS       sequence-82376-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human SRP14 and SRP9-3A proteins as a fusion protein in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  TITLE     Strub Lab 14-9VN plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p14-9VN9-3A
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..47
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             319..327
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      774..783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(825..846)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 accgtcagaa ttgatctacc atggactaca aagacgatga cgacaagctt gcggccgcga
       61 attctatggt gttgttggag agcgagcagt tcctgacgga gctgaccaga cttttccaga
      121 agtgtcggac gtcgggcagc gtctatatca ccttgaagaa gtatgacggt cgaaccaaac
      181 ccattccaaa gaaaggtact gtggagggct ttgagcccgc agacaacaag tgtctgttaa
      241 gagctaccga tgggaagaag aagatcagca ctgtggtgag ctccaaggaa gtgaataagt
      301 ttcagatggc ttattcaaac ctccttagag ctaacatgga tgggttgaag aagagagaca
      361 aaaagaacaa aactaagaag accaaagcag cagcaacagc agcagcagca gcacctgccg
      421 cagcagcaac agcagcaaca acagcagcaa caacagcagc aacagcagca cagtcccccg
      481 ggggaatgcc gcagtaccag acctgggagg agttcagccg cgctgccgag aagctttacc
      541 tcgctgaccc tatgaaggca cgtgtggttc tcaaatatag gcattctgat gggaacttgt
      601 gtgttaaagc aacagatgat ttagtttgtt tggtgtataa aacagaccaa gctcaagatg
      661 tagcagcaat tgaggccttc cacagtcaac taatgcgact tatggtagcc aaggaagccc
      721 gcaatgttac catggaaact gagggggtac cagtcgactc tagaagatcc atcgccacca
      781 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      841 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      901 gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc tgg
//
