LOCUS       sequence-82377-e        6844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ae aegypti PUb promoter Single Stranded Annealing  reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Aryan A, Anderson MA, Myles KM, Adelman ZN
  TITLE     TALEN-based gene disruption in the dengue vector Aedes aegypti.
  JOURNAL   PLoS One. 2013;8(3):e60082. doi: 10.1371/journal.pone.0060082. Epub 
            2013 Mar 21.
  PUBMED    23555893
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(3):e60082. doi"; pages: " 
            10.1371/journal.pone.0060082. Epub 2013 Mar 21"
COMMENT     Sequence Label: pGL3-PUb-SSA
FEATURES             Location/Qualifiers
     source          1..6844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2114..3766
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3807..3928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3818..3837)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3872..3891
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4176..4193)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4347..4935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4427..4446)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5106..5897,5898..5966))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5729..5748
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5967..6071)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6098..6553
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6185..6204)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6395..6416
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    6684..6732
                     /note="synthetic polyadenylation signal"
     misc_feature    6746..6837
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6786..6805
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt atctttacat gtagcttgtg cattgaatcc aattataatt
       61 tgccttggca ccagctgagc cagacaagaa agaaagcttc ccagaagtat atcgatttag
      121 aagggttgac gtcacttgct gactgcacta atacagcaaa tgatgcaatt agaatgattc
      181 aagtgaaatt cccaaattac tgatttttct ctggatttgg ttatcagatt acattcgaag
      241 ctaagattag ctaccgaaat tgtcgatcaa atcaggaaat cctttctcta tcgaaaaagg
      301 cattcgcaca tcttcctctc tatgccatat acacgaaggg taggtacatt gacgtctttg
      361 ccagaagttg aactgcatcg ttcaaggtac agaatgaacg actaacagac acaagcacgt
      421 tttgctgtcc attcagacac agggatggta cccatattcg atcgatatag agccatccaa
      481 ccgaacagag gtatatgtat gaatgtattg ctgaaatttt ctagaagtac aaccaccact
      541 acgacagtgt ctataaaacg cccctgcaaa ggcgaaacca gctcaatcga atacgtttcc
      601 tagtggagtg aacattacgc ggcccaagta agcagtgcca gtgcaagtga agtgaagtct
      661 ctagtgaaaa agagtgatcc aattagccag aggagaaaat ttcagagtga acaaagcttt
      721 attcaaagga caattactat taaattggtg aaagtgcatt tcggtgaagg gaatcttcta
      781 gtgaaggtag gtaaattaat tgatgaaatt atagctatga gcgaaaacta gtttggtgaa
      841 tgattccttt gtctttgaat gagcaaacta ttttccaaga tggcgactat tgagctttga
      901 gtgattagtg aaaatttgca acgcagtttc atcatcattg ataaaaccca attgtgattc
      961 acagcgataa tcatatttcg ttgaatcatc gctactaatt gaattaaatt tctagaataa
     1021 taagaataac gtatttgctc cgtcacatat ctaaaataaa tattttgatg gtaattaccc
     1081 attaaggtaa tattaacaca tatcgagaaa aaccttgagg aaatcgtgaa aacttgaaga
     1141 tacgcaattt caaaactacg tagttcaaag tcgaaaacaa gttaattttt cacttaaaag
     1201 tagggcgttg ttgtgacgtc atcaccttca agtgtatatt tttcacttgg cctgcgactg
     1261 caaacgcaga caaagcaaaa caagtttaaa acctgtcgtg tcgtgctcga agccaaaggc
     1321 aatgaatcaa tatcaaatga gagtttgcat ttcacaacca attactcaag cgtttcctcg
     1381 tttctttttc tgctcaacag agatttcaac catggaagac gccaaaaaca taaagaaagg
     1441 cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat
     1501 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga
     1561 catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
     1621 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
     1681 gccggtgttg ggcgcgttat ttatcggagt aattgattag agatcttccc atcgtgctgc
     1741 ctaacggggg atccgaaata gaacttgctc tactgactgc agttatttga ggaggtttct
     1801 ctgtaaataa tggaatgaat gccggattcg aggattgtac tgtgttgacc gagttgttca
     1861 atcaaccaaa acgtcgtgag acagtttggt cctctcccgg ccctagcaaa gatgaagttc
     1921 tttgtgactt tgggactaat caacgttagg gataacaggg taatagtaca actacaacag
     1981 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2041 caataatcgt agcagctcac ggttaacacc atcttcttca aggacgacgg catgattaat
     2101 taagctcgag accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
     2161 gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
     2221 tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta
     2281 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
     2341 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
     2401 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
     2461 cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat
     2521 tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac
     2581 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
     2641 taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat
     2701 gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg
     2761 cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
     2821 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
     2881 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag
     2941 ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc
     3001 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg
     3061 cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat
     3121 caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga
     3181 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct
     3241 ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat
     3301 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg
     3361 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga
     3421 ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc
     3481 catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga
     3541 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa
     3601 agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt
     3661 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
     3721 agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agagtcgggg
     3781 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     3841 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3901 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     3961 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     4021 aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     4081 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     4141 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4201 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4261 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4321 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4381 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4441 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4501 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4561 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4621 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4681 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4741 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4801 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4861 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4921 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4981 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5041 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5101 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5161 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5221 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5281 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5341 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5401 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5461 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5521 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5581 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5641 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5701 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5761 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5821 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5881 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5941 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6001 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6061 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     6121 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6181 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6241 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6301 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6361 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6421 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6481 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6541 acgcttacaa tttgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     6601 cgggcctctt cgctattacg ccagcccaag ctaccatgat aagtaagtaa tattaaggta
     6661 cgggaggtac ttggagcggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
     6721 gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa
     6781 acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctatc
     6841 gata
//
