LOCUS       sequence-82379-e       19626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector for homologous recombination in mouse ES cells; 
            Rosa26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19626)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 19626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: 
            pBT314_pR26-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..19626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1087
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     protein_bind    1245..1344
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     promoter        1514..1618
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1620..1977
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1620..1811
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1643..1663)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1828..1963
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2012..2806
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2012..2806
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2066..2085)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2676..2695
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2994..3013)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3038..3085
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     RBS             3655..3663
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      8679..8688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8685..10499
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             10500..10520
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10553..10777
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    10863..15132
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        15181..15674
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     15607..15626
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             15701..16270
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphteria toxin Fragment A"
                     /label=DTA
                     /translation="DDVVILLIFCMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDD
                     DWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELG
                     LSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEIN
                     FETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          16347..16412
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    16486..16710
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(16778..16798)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(16778..16796)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(16817..16833)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16817..16833)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(16830..16852)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    16841..16857
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16865..16871,16872..16889,16890..16895))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16910..16931
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(17048..17065)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17219..17807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17299..17318)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17978..18769,18770..18838))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     18601..18620
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18839..18943)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(18969..19424)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(19106..19127)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     19318..19337
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     19551..19573
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     19565..19582
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     19566..19582
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     19592..19611
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        19592..19610
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggascgctt ccggccgacg tctcgtcgct gattggctty ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgn acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttgcccc ctcttcccct cgtgatctgc aactccagtc
     1081 tttctagcct taattaattt aaataggaga tcatgcatcc agatctggtg ataggtggca
     1141 agtggtattc cgtaaggata tcggatcttt tctcgactag tactgacgga cacaccgaag
     1201 ccccggcggc aaccctcagc ggatgccccg gggcttcacg ttttcccagg tcagaagcgg
     1261 ttttcgggag tagtgcccca actggggtaa cctttgagtt ctctcagttg ggggcgtagg
     1321 gtcgccgaca tgacacaagg ggttgtgacc ggggtggaca cgtacgcggg tgcttacgac
     1381 cgtcagtcgc gcgagcgcga ctagtcgaga aggatccttt gtcgacgaag ttcctattcc
     1441 gaagttccta ttcttcaaaa ggtataggaa cttcgctagc gattattcag gtggcacttt
     1501 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1561 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     1621 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     1681 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     1741 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     1801 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     1861 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     1921 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     1981 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     2041 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     2101 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     2161 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     2221 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     2281 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     2341 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     2401 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     2461 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     2521 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     2581 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     2641 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     2701 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     2761 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     2821 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     2881 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     2941 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     3001 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     3061 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     3121 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     3181 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     3241 cctgccatag cctcaggtta ctcaggatca tctctagcct gcaggtgtgc caccatgcct
     3301 ggcccagttt cttgctttgt attataaact ttatagctgg taagactctg gacccacacg
     3361 tgtttgattt gtctcttctc tcctgagaca ttttttccca gtaacatttc ctgaattttg
     3421 tattttattg tctctgcatc tgtgctgaga cataacatct tctgtttctt ctatttgcca
     3481 tgaactggga gttaagtcta agaatttgat taaagctgaa gttgagtttg aaatcaaacc
     3541 tctacatgta gcgttttgta tttcccactg tgatacatca accagcacat ggttcttgga
     3601 ggtctttgct ttgtggtata aagttggccc agtgtgttta aatgttgtcc tctttaagga
     3661 ggtagctagg taagatcaca cgccggttac ctacctccct actgatgcac taactggtgt
     3721 gtctagccta gcctaaggcc ctagggtacc atgcatccca taatagatta tgtgaggccc
     3781 aacacatttg tagataacag catcaggtca tagtgtcata aggttggaca cccctggcag
     3841 agtttcatgc accacactat cttcaaaaga gctaaagaag taaggtggaa aaaacaaaca
     3901 tcatagtgtt ctgattttac tgtaacagga aaagactcac tgaaaagatg ccaaggccca
     3961 gatttcccct cccacaattc caagcttcat ggagaatgat acctccactt tggggatgga
     4021 gaagggatta aatcctgacc acagacttga agcagtaaga acttatcacc gtgatgtcag
     4081 ttctgggtgg aaccctatgc atcccgtggc cctgtacctg cagatgctgc tgctggacct
     4141 gtgatagatg gccagtacta cagttaggaa tctttccgac aactgcagct tttagaatgg
     4201 aaagcaaaac tccacctact ggatggcaca acatgaaagc aaggactgtg ccttgcagct
     4261 cagggccagc cttagtacta gtctctgagg gaccgttgct accaccggtt cccaacctgt
     4321 agcagcacaa ctatcctgga ccagggttca cggatggagc tcaagacctt gacatctgtc
     4381 acagttatgc tctgcaccat gaggaagttg tctcagtttg ccttactgcc acagcaccgg
     4441 ctgtgggctc agcattgtga cactcagagc cacagttctg gagcaggaac tagggcaggt
     4501 ccctctggtg attctttcta cagttcacga ccagaggctg ttggctttgg aaggcacctg
     4561 gaggtctgtg caagccccgg acagaggctt gtgtagatga aggacccttt ataaaaagct
     4621 ccctaattga gtcttgaaaa actcacccgc tgaggattta ggagaaacct aagcttggat
     4681 cgcctcctaa cactgccaca ggtaacctga attttggtgc catatagtgt tagaaactat
     4741 aggctgagag agagaaaaaa aaaacattct tttttcaatt tctgaaaaca aacaaaacca
     4801 aaacaagcca tattatgaag accggaataa atacctaatc ctttgtcctc gaatgacatc
     4861 acacatcaat aagaaccaag gatgtttaag gaactatgac ctcatctgac agtataaata
     4921 taaaatgcca gaaaccgtca agatagtaac cagtgtgggt ggatgctcat atgtgtactg
     4981 tattagttac tttttcattg ttgtgataaa ataccacgac taaagcctga cttataggag
     5041 aaggaatttg tcctgtgttt tcagagtgat aagactccat catggcaggg acgcatggaa
     5101 gccagcaaca ggtatgatgc tggggcagca agctgagagc tcacatccac cactgaaggc
     5161 acaaaacagc aagtgtgagc tggaagtggt gtgaggctat atactagcaa aggctgccct
     5221 actgatacgc tgtctccagg aggactgcgt tccccaagct tctctgatca tcacaaccaa
     5281 ctgaggacta aatgttcaaa tacctgggtc agtggggatc atttctcatt caaacttcca
     5341 cagataatta aaagcaaata aatgaaccag gtttaaggaa acttactgaa ttccaataga
     5401 aaatatagaa ataaatcaat actttagtag atataattga ctgagacatg ggggcaaatt
     5461 ttaaaaagtt gaaattcttg ataatgaagg tatactaaac aaaataaaat tgcattaggg
     5521 aagcattgat gttagagtaa atcaagcaga agagagaatg agtgaattcc aagataggcc
     5581 atttgaaaat gcacagaaga gaaaaagaat taagaggaag aaatgtactc atggtaacct
     5641 tggagcaaca gtgaaagggt aagtaggtta gtggggatat ggacgggact tgagaacacc
     5701 aaaggagtta atcgatgcaa ctacgttatg atcaaccaca gtcaggttcc aggttctgca
     5761 gtaaactgaa tctccagttt tcacgctatg tagatccatc tccatgaatc tggcctggcc
     5821 ttgtgacttt ctggtaactg attagttgaa agttgcaaat ataattgttt aacttcttat
     5881 gctagaattc aagaagcctt gcaactatta ccctggtcct ttggagcgat gtccctgaca
     5941 agtgtgtcca gttgaaatgc agcatcctta cagagtctga ctgagattgt ccaacaacct
     6001 tcagagccta agacagcctc ttggaaacta ttcaattgtt ctggccatcc cagcccctag
     6061 atgccagagt agagaagtca ccttggatgt taaacaaact ggtattggag gttggggata
     6121 aactcagtag tacagagctt gcctggcacg ctcaagtacc taggttcaaa ctctggcaat
     6181 gccaaaaaag aaaaaagttg atagcaaact ctcagatgat tcaggttcta gttagcgttt
     6241 gaatggaatt gtatgagatg gtctcctgag ccctctcaat ccctgagaga gaattttttt
     6301 gtaagtcaat atattatgca ataatttgtt atacagttgt actggctaat ttgggtcaag
     6361 ctggagttat cacagagaaa ggagcttcag ttggggaaat gcctccatga gatccaactg
     6421 taaggcattt tctcaattag tgatcaaggg ggaaaggccc cttgtgggta ggaccatctc
     6481 tgggctggta gtcttggttc tataagagag caggctgagc aagccaggtg aggcaagcca
     6541 gtaaggaaca tccctccatg gcctctgtat cagctcctgc tcctgacctg cttgagttcc
     6601 agtcctgact tcctttggtg atgaacagca atgtggaaag tgtaagctga ataaaccctt
     6661 tcctccccaa tttgcttagt ggtcatgatg ttttgtccag gaatagaaac cctgactaag
     6721 acaatagtaa taggtaacaa gaagagagct gtgaactcgt catgtagtta agataagtgt
     6781 ttgctagatt tcctattgca tagttcctat tttctcttta tatttggaaa aaaattgtgg
     6841 taacatacct tggacactta ttgttgttct cccccctgac ctgcactcaa tgctgaattc
     6901 tcagccaaac tctagtgatg tcctccccat tcaaaagctt caaccttcta gtctttattt
     6961 aaaaaaatgt tattattaca tttatttatt ctgtggtgtg tgtgtgtgag agagaggatg
     7021 ggagcaggtg tgtgtgtgtg tgtgtcggtg tgtttgttaa atcagaacat gagtgtgtgt
     7081 gtaagacaga gacggggtga gtgtgtgtgt gtgtgtatgg gagcaggtgt gtgtgtgtgt
     7141 gtgggtcagt ggataagttg tggaagtcag ttctttcctt cccttactcg gatctcaggg
     7201 atcaaacgca agtcctcagg ttgccaggta catgtcacct gtctttgctt tattattatt
     7261 tagttctggc ttttgagcct aagtgttatg ttacataggt tgaccttgaa ttagctatgc
     7321 actgagctcc tgatcctcct gcctatatct tccaagtaat ggttacagga atgggccact
     7381 ggacctagca agtgaaccta cctggtcact ccaaaaatgc acagcaaaga atatacgttt
     7441 aaaaataggt tcattttagg aagtttgtcc acattttaaa tgacagttct gttaaagtat
     7501 actgtgtttt gtcctttgtt aaatgtgact tttaaaagca attcacctta atagcctggg
     7561 cgactacagt gctcactgta taaatgctag tgtgtttttg gtgctgaaac agaaaggtgg
     7621 ctctagaaag ctggagttcc tcatctttaa gttccagact gagatatcta gaacttcttc
     7681 aaaaatccag ggaaaggaaa gcgatggaag tgaaaataaa aacaagaaca tgctttacat
     7741 atatttgatt gtgatccctt tggcgggtac taggaaaacc acggatggaa ttttccttct
     7801 agaaagaggt gaggagcagg cagaatgtga acatctactt aatgagctga actggccggt
     7861 gccctcagaa ttgtaaacag gtacaccaca aatccaggcc ttggcagaca gaccaagtct
     7921 tcctctcttc ggttttcttt ttacagactg gctttcttga caacttcaag atggagtctc
     7981 atccttgccc tttttatgga gaggagaagc attgcttcta gttggtttta gtagaggagt
     8041 gaagtgcatt tctcagatac aaagagagca cttgagacgt tcggactcag aatggccaag
     8101 cctggcactt tgggaggtca agaggaggct ggaacggctg ggagagaaag caattagatg
     8161 ttccacccct ttggtttttc tccagacagg gtttctctgt gtagccctga ctgtcctgaa
     8221 gtttgctctg tagaccaggc tggcctccac ccagggattt gcctgcctct gcctccccga
     8281 gccccagatt tttattttta tttatatata tattaacttt tgaatgaaca caatggaatt
     8341 gatgagccct tggagagtga aacgggatgt cgtgcgtggc agccccgggg atcagctcac
     8401 tcccacaggc ctcacaggcc atggagccaa gaggcctccc tgcctcggcc tcggcctcgg
     8461 cctcaacaaa ggtggagaac gcgcaggccg tccgtccatg gggcgggaag tcgcgcgccg
     8521 cggccgctga ttggctggcg ggcccggtcg cctgatgcta tttgttgtcc ccgcgccaat
     8581 gacgcagtcg gcgtccggcg tccacccgca cgtgcagccg tttaagccgc gtcggccggc
     8641 cgcgaggagc ccggggagcc tggagcggag agttaaccgc caccatggat acctacgccg
     8701 gagcctacga cagacagagc cgggagagag agaacagcag cgccgccagc cccgccaccc
     8761 agagaagcgc caacgaggat aaggccgccg atctgcagag agaggtggag agggacggcg
     8821 gcagattcag atttgtgggc cacttcagcg aggcccctgg caccagcgcc ttcggcaccg
     8881 ccgagagacc cgagttcgag agaatcctga acgagtgtag ggccggcagg ctgaacatga
     8941 tcatcgtgta cgacgtgtcc cggttcagca ggctgaaggt gatggacgcc atccctatcg
     9001 tgtccgagct gctggccctg ggcgtgacca tcgtgtccac ccaggaaggc gtctttagac
     9061 agggcaacgt gatggacctg atccacctga tcatgaggct ggacgccagc cacaaggaga
     9121 gcagcctgaa gagcgccaag atcctggaca ccaagaacct gcagagggag ctgggcggct
     9181 atgtgggcgg caaggccccc tacggcttcg agctggtgtc cgagaccaag gagatcaccc
     9241 ggaacggcag gatggtgaac gtggtgatca acaagctggc ccacagcacc acccccctga
     9301 ccggcccctt cgagtttgag cccgacgtga tcaggtggtg gtggcgggag atcaagaccc
     9361 acaagcacct gcctttcaag cccggcagcc aggccgccat ccaccccggc agcatcaccg
     9421 gcctgtgtaa gagaatggac gccgacgccg tgcccaccag aggcgagacc atcggcaaga
     9481 aaaccgccag cagcgcctgg gaccccgcca ccgtgatgag aatcctgagg gaccctagga
     9541 tcgccggctt cgccgccgag gtgatctaca agaagaagcc cgacggcacc cccaccacca
     9601 agatcgaggg ctacagaatc cagagagacc ccatcaccct gagacctgtg gagctggact
     9661 gtggccctat catcgagcct gccgagtggt acgagctgca ggcctggctg gacggcagag
     9721 gcagaggcaa gggcctgagc agaggccagg ccatcctgag cgccatggac aagctgtact
     9781 gtgagtgtgg cgccgtgatg accagcaaga gaggcgagga gagcatcaag gacagctacc
     9841 ggtgccggag aagaaaggtg gtggacccca gcgcccctgg ccagcacgag ggcacctgta
     9901 atgtgagcat ggccgccctg gacaagttcg tggccgagcg gatcttcaac aagatccggc
     9961 acgccgaggg cgacgaggag accctggccc tgctgtggga ggccgccaga agattcggca
    10021 agctgaccga ggcccccgag aagagcggcg agagggccaa cctggtggcc gagagagccg
    10081 acgccctgaa cgccctggag gagctgtacg aggacagagc cgccggagcc tatgacggcc
    10141 ctgtgggcag gaagcacttc agaaagcagc aggccgccct gaccctgaga cagcagggcg
    10201 ccgaggaaag actggccgag ctggaggccg ccgaggcccc taagctgccc ctggatcagt
    10261 ggttccccga ggatgccgac gccgacccca ccggccccaa gtcctggtgg ggcagagcca
    10321 gcgtggacga caagagggtg ttcgtgggcc tgttcgtgga taagatcgtg gtgaccaaga
    10381 gcaccaccgg caggggccag ggcaccccca tcgagaagag agccagcatc acctgggcca
    10441 agcctcccac cgacgacgac gaggatgacg cccaggacgg caccgaggac gtggccgccc
    10501 ctaagaaaaa gcggaaagtg tgatagatct agagctcgct gatcagcctc gactgtgcct
    10561 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
    10621 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
    10681 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
    10741 aatagcaggc atgctgggga tgcggtgggc tctatggacg tcctagagaa gttcctattc
    10801 cgaagttcct attcttcaaa aggtatagga acttcgcggc cgcctagtaa ggggcgcgcc
    10861 ggctagaaga tgggcgggag tcttctgggc aggcttaaag gctaacctgg tgtgtgggcg
    10921 ttgtcctgca ggggaattga acaggtgtaa aattggaggg acaagacttc ccacagattt
    10981 tcggttttgt cgggaagttt tttaataggg gcaaatagga aaatggagga taggagtcat
    11041 ctggggttta tgcagcaaaa ctacaggtat attgcttgta tccgcctcgg agatttccat
    11101 gaggagataa agacatgtca cccgagttta tactctcctg cttagatcct actacagtat
    11161 gaaatacagt gtygcgaggt agactatgta agcagattta atcattttaa agagcccagt
    11221 acttcatatc catttctccc gctccttctg cagccttatc aaaaggtatt tagaacactc
    11281 attttagccc cattttcatt tattatactg gcttatccaa cccctagaca gagcattggc
    11341 attttccctt tcctgatctt agaagtctga tgactcatga aaccagacag attagttaca
    11401 tacaccacaa atcgaggctg tagctggggc ctcaacactg cagttctttt ataactcctt
    11461 agtacacttt ttgttgatcc tttgccttga tccttaattt tcagtgtcta tcacctctcc
    11521 cgtcaggtgg tgttccacat ttgggcctat tctcagtcca gggagtttta caacaataga
    11581 tgtattgaga atccaaccta aagcttaact ttccactccc atgaatgcct ctctcctttt
    11641 tctccattat aactgagcta twaccattaa tggtttcagg tggatgtctc ctcccccaat
    11701 atacctgatg tatctacata ttgccaggct gatattttaa gacatwaaag gtatatttca
    11761 ttattgagcc acatggtatt gattactgct actaaaattt tgtcattgta cacatctgta
    11821 aaaggtggtt ccttttggaa tgcaaagttc aggtgtttgt tgtctttcct gacctaaggt
    11881 cttgtgagct tgtatttttt ctatttaagc agtgctttct cttggactgg cttgactcat
    11941 ggcattctac acgttattgc tggtctaaat gtgattttgc caagcttctt caggacctat
    12001 aattttgctt gacttgtagc caaacacaag taaaatgatt aagcaacaaa tgtatttgtg
    12061 aagcttggtt tttaggttgt tgtgttgtgt gtgcttgtgc tctataataa tactatccag
    12121 gggctggaga ggtggctcgg agttcaagag cacagactgc tcttccagaa gtcctgagtt
    12181 caattcccag caaccacatg gtggctcaca accatctgta atgggatctg atgccctctt
    12241 ctggtgtgtc tgaagaccac aagtgtattc acattaaata aataatcctc cttcttcttc
    12301 tttttttttt tttaaagaga atwctgtctc cagtagaatt actgaagtaa tgaaatactt
    12361 tgtgtttgtt ccaatatggw agccaataat caaatactct twagcactgg aaatgtacca
    12421 aggaactatt ttatttaagt gwactgtgga cagaggagcc ataactgcag acttgtggga
    12481 tacagaagac caatgcagac ttaatgtctt ttctcttaca ctaagcaata aagaaataaa
    12541 aattgaactt ctagtatcct atttgttaaa ctgctagctt tactaacttt tgtgcttcat
    12601 ctatacaaag ctgaaagcta agtctgcagc cattactaaa catgaaagca agtaatgata
    12661 attttggatt tcaaaaatgt agggccagag tttagccagc cagtggtggt gcttgccttt
    12721 atgccttaat cccagcactc tggaggcaga gacaggcaga tctctgagtt tgagcccagc
    12781 ctggtctaca catcaagttc tatctaggat agccaggaat acacacagaa accctgttgg
    12841 ggaggggggc tctgagattt cataaaatta taattgaagc attccctaat gagccactat
    12901 ggatgtggct aaatccgtct acctttctga tgagatttgg gtattatttt ttctgtctct
    12961 gctgttggtt gggtcttttg acactgtggg ctttcttaaa gcctccttcc ctgccatgtg
    13021 gtctcttgtt tgctactaac ttcccatggc ttaaatggca tggctttttg ccttctaagg
    13081 gcagctgctg agwtttgcag cctgatttcc agggtggggt tgggaaatct ttcaaacact
    13141 aaaattgtcc tttaattttt ttttaaaaaa tgggttatat aataaacctc ataaaatagt
    13201 tatgaggagt gaggtggact aatattaatg agtccctccc ctataaaaga gctattaagg
    13261 ctttttgtct tatactaact ttttttttaa atgtggtatc tttagaacca agggtcttag
    13321 agttttagta tacagaaact gttgcatcgc ttaatcagat tttctagttt caaatccaga
    13381 gaatccaaat tcttcacagc caaagtcaaa ttaagaattt ctgactttaa tgttatttgc
    13441 tactgtgaat ataaaatgat agcttttcct gaggcagggt ctcactatgt atctctgcct
    13501 gatctgcaac aagatatgta gactaaagtt ctgcctgctt ttgtctcctg aatactaagg
    13561 ttaaaatgta gtaatacttt tggaacttgc aggtcagatt cttttatagg ggacacacta
    13621 agggagcttg ggtgatagtt ggtaaatgtg tttaagtgat gaaaacttga attattatca
    13681 ccgcaaccta ctttttaaaa aaaaaagcca ggcctgttag agcatgctaa gggatcccta
    13741 ggacttgctg agcacacaag agtagtactt ggcaggctcc tggtgagagc atatttcaaa
    13801 aaacaaggca gacaaccaag aaactacagt aaggttacct gtctttaacc atctgcatat
    13861 acacagggat attaaaatat tccaaataat atttcattca agttttcccc catcaaattg
    13921 ggacatggat ttctccggtg aataggcaga gttggaaact aaacaaatgt tggttttgtg
    13981 atttgtgaaa ttgttttcaa gtgatagtta aagcccatga gatacagaac aaagctgcta
    14041 tttcgaggtc tcttggttat actcagaagc acttctttgg gtttccctgc actatcctga
    14101 tcatgtgcta ggcctwcctt aggctgattg ttgttcaaat aacttaagtt tcctgtcagg
    14161 tgatgtcata tgatttcata tatcaaggca aaacatgtta tatatgttaa acatttgkac
    14221 ttaatgtgaa agttaggtct ttgtgggttt tgattttaat ttcaaaacct gagctaaata
    14281 agtcatttta catgtcttac atttggtgaa ttgtatattg tggtttgcag gcaagactct
    14341 ctgacctagt aaccctccta tagagcactt tgctgggtca caagtctagg agtcaagcat
    14401 ttcaccttga agttgagacg ttttgttagt gtatactagt tatatgttgg aggacatgtt
    14461 tatccagaag atattcagga ctatttttga ctgggctaag gaattgattc tgattagcac
    14521 tgttagtgag cattgagtgg cctttaggct tgaattggag tcacttgtat atctcaaata
    14581 atgctggcct tttttwaaaa gcccttgttc tttatcaccc tgttttctac ataatttttg
    14641 ttcaaagaaa tacttgtttg gatctccttt tgacaacaat agcatgtttt caagccatat
    14701 tttttttcct tttttttttt ttttttggtt tttcgagaca gggtttctct gtatagccct
    14761 ggctgtcctg gaactcactt tgtagaccag gctggcctcg aactcagaaa tccgcctgcc
    14821 tctgcctcct gagtgccggg attaaaggcg tgcaccacca cgcctggcta agttggatat
    14881 tttgtatata actataacca atactaactc cactgggtgg atttttaatt cagtcagtag
    14941 tcttaagtgg tctttattgg cccttattaa aatctactgt tcactctaac agaggctgtt
    15001 ggactagtgg sactaagcaa cttcctacgg atatactagc agataagggt cagggataga
    15061 aactagtcta gcgttttgta tacctaccag cttatactac cttgttctga tagaaatatt
    15121 taggacatct agcttatcga tccgtcgacg gtatcgataa gcttgatatc gaattctacc
    15181 gggtagggga ggcgcttttc caaggcagtc tgagcatgcg cttagcagcc ccgctggcac
    15241 ttggcgctac acaagtggcc tytggcctcg cacacattcc acatccaccg gtaggcgcca
    15301 accggctccg ttctttggtg gccccttcgc gccaccttct wctcctcccc tagtcaggaa
    15361 gttccccccc gccccgcagc tcgcgtcgts aggacgtgac aaatggaagt agcacgtctc
    15421 actagtctcg tcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
    15481 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
    15541 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
    15601 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
    15661 ctccgggcct ttcgacctgc aggtcctcgc catggatcct gatgatgttg ttattcttct
    15721 aatcttttgt atggaaaact tttcttcgta ccacgggact aaacctggtt atgtagattc
    15781 cattcaaaaa ggtatacaaa agccaaaatc tggtacacaa ggaaattatg acgatgattg
    15841 gaaagggttt tatagtaccg acaataaata cgacgctgcg ggatactctg tagataatga
    15901 aaacccgctc tctggaaaag ctggaggcgt ggtcaaagtg acgtatccag gactgacgaa
    15961 ggttctcgca ctaaaagtgg ataatgccga aactattaag aaagagttag gtttaagtct
    16021 cactgaaccg ttgatggagc aagtcggaac ggaagagttt atcaaaaggt tcggtgatgg
    16081 tgcttcgcgt gtagtgctca gccttccctt cgctgagggg agttctagcg ttgaatatat
    16141 taataactgg gaacaggcga aagcgttaag cgtagaactt gagattaatt ttgaaacccg
    16201 tggaaaacgt ggccaagatg cgatgtatga gtatatggct caagcctgtg caggaaatcg
    16261 tgtcaggcga tctctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
    16321 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
    16381 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
    16441 agcagtggtg gaatgcagat cctagagctc gctgatcagc ctcgactgtg ccttctagtt
    16501 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
    16561 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
    16621 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
    16681 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
    16741 cgacctcgag ggggggcccg gtacccagct tttgttccct ttagtgaggg ttaattgcgc
    16801 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    16861 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    16921 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    16981 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    17041 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    17101 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    17161 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    17221 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    17281 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    17341 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    17401 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    17461 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    17521 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    17581 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    17641 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
    17701 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    17761 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    17821 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    17881 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    17941 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    18001 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    18061 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    18121 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    18181 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    18241 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    18301 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    18361 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    18421 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    18481 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    18541 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    18601 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    18661 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    18721 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    18781 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    18841 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    18901 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    18961 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
    19021 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
    19081 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
    19141 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
    19201 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
    19261 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
    19321 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
    19381 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
    19441 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    19501 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    19561 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattgga
    19621 gctcca
//
