LOCUS       sequence-82385-e        5779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosophila pseudoobscura hsp82 promoter driving firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Aryan A, Anderson MA, Myles KM, Adelman ZN
  TITLE     Germline excision of transgenes in Aedes aegypti by homing 
            endonucleases.
  JOURNAL   Sci Rep. 2013;3:1603. doi: 10.1038/srep01603.
  PUBMED    23549343
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013"; volume: "3:1603. doi"; pages: " 10.1038/srep01603"
COMMENT     Sequence Label: pGL3-hsp82
FEATURES             Location/Qualifiers
     source          1..5779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1049..2701
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1115..1133)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2742..2863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2753..2772)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2807..2826
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3111..3128)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3282..3870)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3362..3381)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4041..4832,4833..4901))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4664..4683
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4902..5006)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5033..5488
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5120..5139)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5330..5351
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5619..5667
                     /note="synthetic polyadenylation signal"
     misc_feature    5681..5772
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5721..5740
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttat
       61 ggatttttac catattatta ttatttctag ccacgttgca actctatgtc agtaccggaa
      121 atagcagccc tggagtctct tagcctctag aaacggctag aacattctac gcttgtggtt
      181 ggttttcatt gaaagcaggc gtcttttata tactttacgg tatatagcta catgtatata
      241 atggtatact tcatcaatat catcaatcta tgaattttaa tttttaagag tacatatata
      301 aattaacatg ggggatatag ttctcaatac ccaagtattt gaattttcca tctctcatcg
      361 ggggtaattc atgaaccggt tccagccgaa aaatgaacga aattcatgag agattatttt
      421 ttcgggattg cttgccaata catttcggaa aaacaaaatg tactacattt ttgtcatctc
      481 agggtgctcc aattaattat gaatgctacg acactacaaa gcagcttgga aatccgaatt
      541 ttaacaataa ttaaaggaaa tagggtatag cgtatatagg gtatcatagc tgaaacgggt
      601 ataccaacaa taatgacgca gcacttacgt ttcactccgt actcacttac gatttatgct
      661 tataattttt gttcacctct tttacttaaa cctcacttta aaaacaatca aataaatggg
      721 agtatttatg tatatttcta agattacggc ggtattgttc tgctgtctgc ggtcacactg
      781 gttttcagcc tcggtgcaac tctgtttcag taccggaaat agcagccctg gattctcgta
      841 gcctctagaa acgtctagaa aattctacgc ttggggttgg tttgctataa aagcaggcgg
      901 gccgactgtt gccggctcga gtcttgaaaa atttttgtcc agtgaaggtg cgtttgctta
      961 gagcgcagtg caacaaagtg aatttattct acacaaatcg aagtgaaaat atatatatat
     1021 ttttatctct gctgttaaat taaaacccat ggaagacgcc aaaaacataa agaaaggccc
     1081 ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa
     1141 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat
     1201 cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg
     1261 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc
     1321 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga
     1381 acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa
     1441 ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat
     1501 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca
     1561 tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac
     1621 aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc
     1681 tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat
     1741 cattccggat actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac
     1801 tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga
     1861 gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct
     1921 attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga
     1981 aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt
     2041 ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat
     2101 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc
     2161 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg
     2221 tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
     2281 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
     2341 cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc
     2401 cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg
     2461 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa
     2521 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa
     2581 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga
     2641 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta
     2701 attctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     2761 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2821 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     2881 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     2941 ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga gccttcaacc
     3001 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     3061 tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc ctcgctcact
     3121 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3181 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3241 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3301 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3361 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3421 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3481 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3541 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3601 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3661 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3721 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3781 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3841 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3901 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3961 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4021 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4081 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4141 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4201 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4261 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4321 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4381 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4441 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4501 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4561 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4621 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4681 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4741 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4801 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4861 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4921 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4981 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     5041 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5101 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5161 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5221 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5281 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5341 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5401 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5461 tttaacaaaa tattaacgct tacaatttgc cattcgccat tcaggctgcg caactgttgg
     5521 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc ccaagctacc atgataagta
     5581 agtaatatta aggtacggga ggtacttgga gcggccgcaa taaaatatct ttattttcat
     5641 tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa
     5701 acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca
     5761 gaacatttct ctatcgata
//
