LOCUS       sequence-82387-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses human Syndecan-1 serine 15 and 25 mutated to alanine 
            (numbering excludes signal peptide) in HEK293-EBNA1 (293E) 
            suspension culture. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Zhang Y, Wang N, Raab RW, McKown RL, Irwin JA, Kwon I, van Kuppevelt
            TH, Laurie GW
  TITLE     Targeting of heparanase-modified syndecan-1 by prosecretory mitogen 
            lacritin requires conserved core GAGAL plus heparan and chondroitin 
            sulfate as a novel hybrid binding site that enhances selectivity.
  JOURNAL   J Biol Chem. 2013 Apr 26;288(17):12090-101. doi: 
            10.1074/jbc.M112.422717. Epub 2013 Mar 15.
  PUBMED    23504321
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.422717"; journalName: "J Biol Chem"; date: 
            "2013-04-26- 26"; volume: "288"; issue: "17"; pages: "12090-101"
COMMENT     Sequence Label: pTT5-hSDC1-S15/25A
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     510..529
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcc tcactctctt ccgcatcgct gtctgcgagg
       61 gccagctgtt gggctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga
      121 tcggaaaccc gtcggcctcc gaacggtact ccgccaccga gggacctgag cgagtccgca
      181 tcgaccggat cggaaaacct ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg
      241 ctgagcaccg tggcgggcgg cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg
      301 ctgatgatgt aattaaagta ggcggtcttg agacggcgga tggtcgaggt gaggtgtggc
      361 aggcttgaga tccagctgtt ggggtgagta ctccctctca aaagcgggca ttacttctgc
      421 gctaagattg tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgatctggc
      481 catacacttg agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca
      541 ggtccaagtt taaacggatc tctagcgaat tccctctaga gggcccgttt ctgctagcaa
      601 gcttatgagg cgcgcggcgc tctggctctg gctgtgcgcg ctggcgctga gcctgcagct
      661 ggccctgccg caaattgtgg ctactaattt gccccctgaa gatcaagatg gcgctgggga
      721 tgactctgac aacttctccg gcgcaggtgc aggtgctttg caagatatca ccttgtcaca
      781 gcagaccccc tccacttgga aggacacgca gctcctgacg gctattccca cgtctccaga
      841 acccaccggc ctggaggcta cagctgcctc cacctccacc ctgccggctg gagaggggcc
      901 caaggaggga gaggctgtag tcctgccaga agtggagcct ggcctcacc
//
