LOCUS       sequence-82393-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses human Syndecan-1 point mutant at S184A and S194A in 
            HEK293-EBNA1 (293E) suspension culture..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Zhang Y, Wang N, Raab RW, McKown RL, Irwin JA, Kwon I, van Kuppevelt
            TH, Laurie GW
  TITLE     Targeting of heparanase-modified syndecan-1 by prosecretory mitogen 
            lacritin requires conserved core GAGAL plus heparan and chondroitin 
            sulfate as a novel hybrid binding site that enhances selectivity.
  JOURNAL   J Biol Chem. 2013 Apr 26;288(17):12090-101. doi: 
            10.1074/jbc.M112.422717. Epub 2013 Mar 15.
  PUBMED    23504321
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.422717"; journalName: "J Biol Chem"; date: 
            "2013-04-26- 26"; volume: "288"; issue: "17"; pages: "12090-101"
COMMENT     Sequence Label: pTT5-hSDC1-CS1/CS2
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     511..530
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc ctcactctct tccgcatcgc tgtctgcgag
       61 ggccagctgt tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg
      121 atcggaaacc cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc
      181 atcgaccgga tcggaaaacc tctcgagaaa ggcgtctaac cagtcacagt cgcaaggtag
      241 gctgagcacc gtggcgggcg gcagcgggtg gcggtcgggg ttgtttctgg cggaggtgct
      301 gctgatgatg taattaaagt aggcggtctt gagacggcgg atggtcgagg tgaggtgtgg
      361 caggcttgag atccagctgt tggggtgagt actccctctc aaaagcgggc attacttctg
      421 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgatctgg
      481 ccatacactt gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc
      541 aggtccaagt ttaaacggat ctctagcgaa ttccctctag agggcccgtt tctgctagca
      601 agcttatgag gcgcgcggcg ctctggctct ggctgtgcgc gctggcgctg agcctgcagc
      661 tggccctgcc gcaaattgtg gctactaatt tgccccctga agatcaagat ggctctgggg
      721 atgactctga caacttctcc ggctcaggtg caggtgcttt gcaagatatc accttgtcac
      781 agcagacccc ctccacttgg aaggacacgc agctcctgac ggctattcc
//
