LOCUS       sequence-82394-e       13912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for PhiC31 integrase-mediated integration of pHb9-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13912)
  AUTHORS   Tasic B, Hippenmeyer S, Wang C, Gamboa M, Zong H, Chen-Tsai Y, Luo L
  TITLE     Site-specific integrase-mediated transgenesis in mice via pronuclear
            injection.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7902-7. doi: 
            10.1073/pnas.1019507108. Epub 2011 Apr 4.
  PUBMED    21464299
REFERENCE   2  (bases 1 to 13912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019507108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7902-7"
COMMENT     Sequence Label: pBT366_pattB-Hb9-GFP-pA-FRT5
FEATURES             Location/Qualifiers
     source          1..13912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(143..164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     355..374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    695..764
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        1010..1028
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     intron          10262..10491
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     10474..10493
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      10538..10547
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(10544..10546,10547..10549,10550..11260)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(10544..10546,10547..10549,10550..11260)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10589..10610)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10850..10869)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11197..11218
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(11381..11398)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11387..11611
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(11727..11745)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11766..11782)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11766..11782)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11779..11801)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11790..11806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11814..11820,11821..11838,11839..11844))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11859..11880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11997..12014)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12168..12756)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12248..12267)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12927..13718,13719..13787))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13550..13569
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13788..13892)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg atgtaggtca cggtctcgaa gccgcggtgc gggtgccagg
      721 gcgtgccctt gggctccccg ggcgcgtact ccacctcacc catctggtcc atcatgatga
      781 acgggtcgag gtggcggtag ttgatcccgg cgaacgcgcg gcgcaccggg aagccctcgc
      841 cctcgaaacc gctgggcgcg gtggtcacgg tgagcacggg acgtgcgacg gcgtcggcgg
      901 gtgcggatac gcggggcagc gtcagcgggt tctcgacggt cacggcgggc atgtcgacgg
      961 tatcgataag cttcccttaa ttaaccgttt tcgaggcggc cgcgagctca attaaccctc
     1021 actaaaggga gtcgactcga tcctgtctcc ctggccagga gtctggggtc cgcagatata
     1081 ttacaacaaa attactaatt ggaaaatgaa attgtcaata aagcacaccc cacgcgtgta
     1141 ctatatcttt aaatattatg atgtcttatt ggtcccattt ccttacaata atttattgca
     1201 ataaaacaga cactgcaggt ccccttaaat tgtcttttca taatgaataa atttaagcag
     1261 gctaattaat atataaacta gctcaatttg tcaagttgat ttgtatttta gttaattgtg
     1321 aaagtaatta ccacatggtc aaattaacag ctttctggaa atgaccaagc ctgaggtttt
     1381 atttccttcc tgggtgaaga aaattcattt ttccaagctc ttgatgtgat gaataaaagt
     1441 cataaatctg ggtgattggt gcaggcagag tctaaatggc ttcatatttc attttaggtt
     1501 taatagaaat attcatgctc tgttttaatg aaattaaatt gaagggggat ggggctctgg
     1561 aggcggccct ggctgggggc ttaaagggac agtgcacttt tcctttcttc atgcaagggc
     1621 cagggctaga ggtccctgga ctcagtcaag aagtggagag agctgaggga ggcctgggag
     1681 gcccttattc tgtacccagt gtagcacacc tgcagccctg attggggggg ggggggggcg
     1741 ggggcaattc acaccctgta ttctcaccag gctaaagttt cttctatctg gttccttctc
     1801 tccaaaaaat cagtcagaag ggacctccct tggctgagag tagaagtgag gtggtcagcg
     1861 tgctgcagac aagcctgtcc tccatctgcc taaggggtct gatatcctac accaagcccc
     1921 ccctacccct tctggagaca cataggagat aagcattttc aaaaccacca tggcactctc
     1981 ctgttctccc ctgcacccct tcctcccaga ctccatcctg cctgaggatg atttggtcca
     2041 acttgaataa tagagtactc tctttccaca tctttccaca tcggaaagag tagtccggga
     2101 tccagtccac ctgcagccct gattaaagga aggaaggaag actagaggcg cagttctacc
     2161 tattatactt attttaaggt tcccaggtca gagatgcctc atggacattt ctccctaagg
     2221 caggtcctga ccagccctcc ccctgaatgg tgctgatctg tgacttgttg cttctattga
     2281 acaaatacag ctttgatttg tttccagcca gagtggttag ctgatgaatt gacaaaaact
     2341 aatcagcttt attgggaaac aggtttaagg gcacggacgt gtcaataacg ctcagcctga
     2401 ccccctcttc cattagctag gcaggctgat tagagtaaga agaccctctc gccgattgca
     2461 ccgaaattgc tttcataaac tcacaatatt agcagtcgat gtgagcagct aatttagaga
     2521 agacatccag gcccgtcaca tcgatcatcg ggtttatgta tcattatctc atttacagaa
     2581 tgtcgcagtg taatacatgt ttttggcaat aggcttggcc gctcgccgga gccgcgcgct
     2641 gttcctttcg gccacactgc aggggacttc ctcaacaatc tacgatgtct cttcgcgtga
     2701 acaaggaggc actttgcaca catatgcaca cttgcgccca ggctctcagt gcgcgctcag
     2761 tgcacacatg cacgtggaca catgctccga catactacct gagctgcctt tccttctcat
     2821 gcggggatgc tggcattgct ctctcctgcg agcccctggt ctgaagccca atgccggacg
     2881 ctcaccaacc ttggagtcaa cctcggagag ctcaaggttc ggttctgtca ccagcataca
     2941 gagatggtgg gtggcggcgc aatcagccac cttctctcaa ggctttggtc gcccagttgg
     3001 cgggtgtcca gaaatccaca ggcctgcgaa ggtccttctc cgccctccca ggctccagct
     3061 tgacctgccc tgccctgggc gggcctttct cgcaacactt ccaggctcag ccagatgcgc
     3121 ctggtcttag ggagtgggcc acctgttcct ttgggtaatt attttaatgt aaggggtcta
     3181 cttcaggcct cctgcagtga cagacggcag cggagctgca aagcgctctc agcctttcag
     3241 tggaatttcc cagatgggcc aaggggaggc cagggcgggt caccaggggc cctttaacga
     3301 gttcattagc caaattatct tgcctgcagc aaggacccca gagactgtag ggagagtagg
     3361 ggagaaggtg ggtctttctc cctccctgct agtctgcaag aacagttgtg ggaaggaaag
     3421 agaaaggaca tcctccccaa aggggcctag gaacactaaa ggcaaagatg gcatctctga
     3481 tgtgacttat gcgccagacc agcgaggtgg tggagtggtg ggaaggaatc ataatgatta
     3541 ggaaacacgg agattctaac aaccagggca ctgacccata ttgtggaaca aacctagtgc
     3601 agaaatcaaa gtcagagccc ctggaacttt attctgtgct tcttaagcaa agaacctgga
     3661 catcttggag ccttggtctg tccgtccatc tgtagagtag aaggtaggtg tgtttccttc
     3721 cacaatttgt gagatcactg ccctaagaaa agcaactctg ggaagagctt ctggcatgac
     3781 cctgttgggt ccttcgggcc cccttactcc tgtggtagga ggctttggca ggttgaggtg
     3841 agcctgatcc tagatgcctg tgctggaaaa tgaacccaac tctcacctgt cgtcgcgccc
     3901 cccaccccca ccccacagct gtgaggcgct gctctggagg tagctcagca tccccgcccc
     3961 tcctccttgt cctcactctg cctcctgtag tgttagcttc tgctacctca gcaagtagtg
     4021 agtgaataga ccaattagcc aggacttcct gccattatct gctgtagact tgggcattag
     4081 tacgccctga ttcaccataa tttcaagtca gaacacacta aaaaattcac tcaccgaggc
     4141 attaaaatgt tcccagacaa cgtctgtgtt tgtgtaactg ggtttcttcc cttcgtgttt
     4201 gatgcacacc agcatcatat aggtcagtgg tggagtccag gctaggcaga caatttttgt
     4261 cgctttttgt ttgtttttat atttttaaaa catgaattgc attgtttccc ggtggttgta
     4321 aactttgttt ctggggcttc agtattgcca gtgtaatctt cttctgcttg atcaggggca
     4381 aacaagtgac ttagaaagtt gtgggagtgc cctaagaaca gtagccagag cctttacaga
     4441 gccaggcttt gtgtcctaaa cgtacatcgc cccagctatg ggaagctggg ttatcacctt
     4501 caaagcagac cctttctgtg gatgacttac caagccctgc aggtgggtag tggaggtggg
     4561 gagagggctg cacagcctgc ttggagcttg cttctgttct ttgtcctgcc catgatatga
     4621 agctcctata caatgaattt gcagtaagat cttggagcaa aagtacaggt ttcctgagaa
     4681 gtctttcgtt tcttctgagg gtgctgtttt acttacacac acacacacac acacacacac
     4741 acacacacac acacacacac cctagctgat taaaagaact gaggtgacct ctcttagcct
     4801 tgtttctctt tatgattcat acctatggcc tctaggattg taagagaaat tgaaaatgtc
     4861 tttggcttcc tgggtcagaa agcatccagc gcagggctga gctcagtctc ttctctatgt
     4921 tctaagtaca cagcaaaaca aacaaaaacc cgaaaccttg agtaaaattt tcaaatatag
     4981 aatatatttt tgtgctggag tttttcaaat ttatatctta ctgtctttct ccctgtaccc
     5041 ctacaaccca ggtttgggct ggacttattt atttatttac aaggaggaat ggggccagtg
     5101 gagaagggca gccagtgaag atggcctgag ggtggaccag gcagtaggga gggcagagta
     5161 agctttctaa aaagggagga aggaaatcca agtgtgctgg gagaggtaga gaaggggggg
     5221 gggctggcaa agaaaaggac tggcttgtag tgtccttggc aagagcagtt cacctcaggt
     5281 tggaacactg ggtcttcagg tcacctgtct tctgagccac ctttgcaaac caccccttta
     5341 aagctgaact ggggatggag gtctgagaaa agggtggtag atcaaaggat ggtaataaag
     5401 gagctgatgg tacttttggg ctctgaaggg gctgtaatga gtgtgtgtgt gtgggggggg
     5461 actcatcaga catcccttct cctgatctca cctccctgtg accccaggct ccccattgga
     5521 ttctgtgtgt cctcaggctg gggtgggtgg acgggttatg tccccagaga cattccaagc
     5581 actgatttca gtctgacttg aaggaagttg gtctatgacc ttccaggtcc agcattcaca
     5641 cttcctgctt gctttctgtc ctcctgagta cttattcagc ctctgaaaaa taccaccatc
     5701 accacccctg tgggcttgtg tcctggacct cactggcctc actacatcct ccaggctcat
     5761 tttcctgaag ggaagcctta aaatgttttt ttttttaaag atttatttat ttattatatg
     5821 taagtacatt gtagctgtct tcagacacac cagaagaggg agtcagatct cgttacagat
     5881 ggttgtgagc caccatgtgg ttgctgggat ttgaactctg gaccttcgga agagcagtag
     5941 ggtgctctaa cccactgagc catctcacca gccccgcctc aaaatgttga tgtgagttaa
     6001 ctctcatgga gttcaaaagg gctgagaagt cagtcttcat gaacctttct ccatgaggct
     6061 gctgtgggca gggggttgct tgttttaagt tttggaaaca gccagacaaa tggcaggtgt
     6121 gtggagggag atgcagggaa tgctgccttc catccagtct cctagccata cctccaggct
     6181 cttggccttt ttgttaatgg ctattatata ttgtacctgc tgcattccac agaaagggat
     6241 gtgtttgtag gtgatcagtt atagaatcaa tgctgacctg acctgtgaat atgggtgcat
     6301 gtaggttttg ttctgagttc ttgtataatg tcaggggtaa aacttgtaga ttataacatc
     6361 tttgtaagtt ttctttttat gtttgcattg tgtgtatgtt tgtgtctatt tatgggaatg
     6421 cacgtaccca aagaggccat aagagggaat ccgatttcct gtagctggag ttggaggtgg
     6481 ttgtgagtgt gagtggtggg aacccaactt gggtcctcct caagagctgc actcactctt
     6541 aagcactgaa ctgtggaacc actatactcc ccaccccaac atcttatttc cttgtggtta
     6601 tttgtataaa tcaacacacc tttctcagta atgagaggag gctcaatcac aagttatatt
     6661 cagtaactta atgcaagacc caaaagaggg atcctggcta gacgcagaga aaaaaatgac
     6721 tgggagccag atgattaaat gtacaccggg ccttaaattc cagcctcttt tgggtaggag
     6781 aggtcttcct ctctgccctc caagagcctc atttggcccc tggggcttct ggagaaggga
     6841 gctaccttga ggcacaacaa tgggaatgct gtgctcactt cggcagcacc tatactaaaa
     6901 ctgaaccgat acagagaaga ttagcatggc ccctgcgcaa ggatgacatg caaatttgtg
     6961 aagcattcct tatttttttt tgccaggacc aggaatggga acaggtgggt tggggagcag
     7021 agggagggga gaggggatag gggatttggg gaggggaaac taggaaaggg gataacattt
     7081 gaaatgtaaa taaagaaaat atctaataaa aaaaaaaaaa agaataggca tgcttctggg
     7141 accagacctt tgatctctca agggtaccgt agtaaacccc cagctcagtc ccaggctgcc
     7201 ccagagccag ctgggctggg tcaatagacg ctgggaacag gaagagacgc agcatgctca
     7261 ctccactcgt aaaacacaca aagatcccag ttccagacga ctcccagtct ctctcccaac
     7321 tcccacctct caggatacaa gttcccgctc aaaccgaagc agaatatatg atcatattca
     7381 agccctttga cttcagagtc tttttggccc aacccccact ttgtcaaaca aagaaatata
     7441 tccatcaagt ctgatatgca aatttcccct tcgctgaagg ggctcttgat acaacctcac
     7501 tcagcccaca ggtggtgggg actggtgtgg gttagcctgg tggatggtca aacaccagac
     7561 gaagtgggaa gggtttgaaa gggatttaga agcttttgga tcacatcccc tacacaacaa
     7621 acaggaaatg aattcctctt ctcggatgac tttactaagg aaacctgtgc ctaaactcag
     7681 tagacctaat actgtatcat caccaaaaac aaccctggtt gtgtagttta gagatgattc
     7741 gggtccatga ggtccacccc aggaaaatct catctgcagc cacccttttt ttcctccttc
     7801 ttctcctttg tttgttttgt tgttgttttt ttcaagacag ggtatctctg tgtagcattt
     7861 ttggttgctg tcctggaact ggctctgtag actaggctgg cctagaactc acagacccca
     7921 cctgcctctg cctcctgagt gctgggatta gagacttgcc tcaccatggt taggttctct
     7981 ttcttctttg ggactctcat ctggaaaagg cccatgggcc ttaaacacaa gtttctgtaa
     8041 atgcaatggg gtcaagactg aatcctgggg atgggggtag ggcatcccag catccttact
     8101 tgccttaatt gggcttccaa gataggagaa ctggcaggga accactcatg gaacacaagt
     8161 gttcctttaa ccctctaaaa ggcagagctg cattttcatc accccaaaga gacagcacct
     8221 aaagacaaga gctatgtgct tgtactattt tcagactagc tggaggcctt tgcaaagctg
     8281 tgcacaactt actccaagat gttccccgct gggttccaga ttttccagcc aggaccaccc
     8341 tgtcataata aaaactccac tcagccgagc atggtggcgc acacctttaa tcccagcact
     8401 tgggaggcag aggcaggagg atttctgagt tcgaggccag cctggtctac agagtgagtt
     8461 ccaagacagc cagggctaga cagagaaacc ctgtctccaa aatcaaaaac aaaaacaaaa
     8521 caaaaacaac aaaaacaaaa aacctactca ctcatcccaa cagaatcact gcgttaaatc
     8581 tctcctaaac aaaacttgtt gacagggaaa atatagtcct tacttatatt tatcgttacc
     8641 ccccaccccc aatccctctt gaaacagctt tgcctgctac ttttaaattc acccaacaag
     8701 gattgtccta gcctcctggc cttccctggg ccatttccga tcttttatta acctctcacc
     8761 tggttgggaa gccgcgatcc agagacctgg aactccctcc cactttcccc tcgtgtttgg
     8821 ggaagatacc cgagaatgga gctagcgaga aagcttgatt tctcaaacta cttattgatt
     8881 cgttacatta aaaaaaaaaa gcccccggaa gcgtgtttac agtaaatatt tgatatgtgc
     8941 gcaggctgag gtctggattc acactttaat acggtctctg gaagcccttt cttgattttc
     9001 ctgaactcgg ccggcaggag gaggcgagag cctgatagtg gctcccttag cctgcgaact
     9061 ggctcagtcc gagggagcag tcctcggcca attcccgcaa agcctttttc ctacaccccc
     9121 acaaggcaag gctctaatag aacttggttc tcacctaggt ccggctcagt ggcacctgtg
     9181 caaacgacct tcaagtcaat gtgcatctct gagttggaaa actgctctct ctccactctg
     9241 actgaagatt tgtaggcttc aacctctaat accgtattta tacacaccga aaccctacaa
     9301 acaaacattt gcggcgcatg atacttttgg gggaggggca gataagaagg ttcgacctgc
     9361 tgagggttaa tgacagggac gcaaaccatc ttgaagcaat caacactcag gaacgcgaga
     9421 gattttggga aaccgaacag tcttcagtgt actaagctca gggtcccgtt ttgggtacct
     9481 agtggacttc aggggtgagg acccacctag ttcctcagtg cagaacattc cacaactccc
     9541 tcaagggagc gcgagtagct ggaggaagat aaggcgtccg cctctggagg aacaaggtgt
     9601 caaagggact cggggggcac tcactgggac ccagggttac tggcgctcac cactctccca
     9661 gcagcaagga agcagtaagc ttgtgttcag cctccaaact ctccaacctg cctcctccgg
     9721 ccacctacgc tgttcaccac cggctgctta ggacctgggg gcgcaggggc ggggcgtcag
     9781 gctaattgga ccgtgacagg tgagcccctc ggccaatcgc cgggcagaca gccctcctcg
     9841 ggcccccgct gagcgcgcca atcccagacc gccctgagct tcaagtgtgc ggaggggcgc
     9901 gggtccccac caaggcacca gtggcctcga tgcggtcgct tgagctcttg agagcagaga
     9961 gccggtctcc ccaacacaaa ctcgcaggag cgctccggtg gaagttcata ccagtgagtg
    10021 gagcgcggtc cccacgagga ccgcaaagag gaggcgcctg gacgatggtc gctctcagct
    10081 gagtttccgg ctgcgacttt attggcaaaa aatcgcagta acaataccgg ccccaaggct
    10141 ggccaccgcc acgctcagtc tccgtaagcc cgcacacgac tgcatcgccc acccttggac
    10201 cctgattgtc agctccaatc gagccggcgc gcctcgctgt ctgcgagggc cagctgttgg
    10261 ggtgagtact ccctctcaaa agcgggcatg acttctgcgc taagattgtc agtttccaaa
    10321 aacgaggagg atttgatatt cacctggccc gcggtgatgc ctttgagggt ggccgcgtcc
    10381 atctggtcag aaaagacaat ctttttgttg tcaagcttga ggtgtggcag gcttgagatc
    10441 tggccataca cttgagtgac aatgacatcc actttgcctt tctctccaca ggtgtccact
    10501 cccaggtcca actgcagtcc aagcggatca cccggtcgcc accatggtga gcaagggcga
    10561 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
    10621 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
    10681 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
    10741 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
    10801 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
    10861 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
    10921 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
    10981 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
    11041 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
    11101 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
    11161 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
    11221 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca gatctaagct
    11281 gaaccctcct gatgagagtg gccccggctg catgagctgc aagtgtgtgc tctcctgagg
    11341 atcccatggg ggcccgctag ccctagagct cgctgatcag cctcgactgt gccttctagt
    11401 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
    11461 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
    11521 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
    11581 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
    11641 tcgaaattgg gcgcgccgaa gttcctattc ttcaaaaggt ataggaactt cgggccgcca
    11701 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
    11761 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    11821 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    11881 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    11941 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    12001 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    12061 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    12121 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    12181 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    12241 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    12301 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    12361 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    12421 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    12481 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    12541 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    12601 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    12661 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    12721 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    12781 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    12841 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    12901 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    12961 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    13021 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    13081 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    13141 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    13201 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    13261 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    13321 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    13381 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    13441 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    13501 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    13561 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    13621 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    13681 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    13741 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    13801 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    13861 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gc
//
