LOCUS       sequence-82397-e        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of HA tagged HIF1alpha (full length) with 
            P402A,P564A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Kageyama Y, Koshiji M, To KK, Tian YM, Ratcliffe PJ, Huang LE
  TITLE     Leu-574 of human HIF-1alpha is a molecular determinant of prolyl 
            hydroxylation.
  JOURNAL   FASEB J. 2004 Jun;18(9):1028-30. Epub 2004 Apr 14.
  PUBMED    15084514
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J."; 
            date: "2004-06"; volume: "18(9)"; pages: "1028-30. Epub 2004 Apr 14"
COMMENT     Sequence Label: p(HA)HIF1alpha (P402A,P564A)
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             135..161
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             141..167
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccgg gccccccctc gaggtcgacc
      121 gattcaccat ggcgtaccca tacgacgtgc ctgactacgc ctccctcatg gagggcgccg
      181 gcggcgcgaa cgacaagaaa aagataagtt ctgaacgtcg aaaagaaaag tctcgagatg
      241 cagccagatc tcggcgaagt aaagaatctg aagtttttta tgagcttgct catcagttgc
      301 cacttccaca taatgtgagt tcgcatcttg ataaggcctc tgtgatgagg cttaccatca
      361 gctatttgcg tgtgaggaaa cttctggatg ctggtgattt ggatattgaa gatgacatga
      421 aagcacagat gaattgcttt tatttgaaag ccttggatgg ttttgttatg gttctcacag
      481 atgatggtga catgatttac atttctgata atgtgaacaa atacatggga ttaactcagt
      541 ttgaactaac tggacacagt gtgtttgatt ttactcatcc atgtgaccat gaggaaatga
      601 gagaaatgct tacacacaga aatggccttg tgaaaaaggg taaagaacaa aacacacagc
      661 gaagcttttt tctcagaatg aagtgtaccc taactagccg aggaagaact atgaacataa
      721 agtctgcaac atggaaggta ttgcactgca caggccacat tcacgtatat gataccaaca
      781 gtaaccaacc tcagtgtggg tataagaaac cacctatgac ctgcttggtg ctgatttgtg
      841 aacccattcc tcacccatca aatattgaaa ttcctttaga tagcaagact ttcctcagtc
      901 gacacagcct ggatatgaaa ttttcttatt gtgatgaaag aattaccgaa ttgatgggat
      961 atgagccaga agaactttta ggccgctcaa tttatgaata ttatcatgct ttggactctg
     1021 atcatctgac caaaactcat catgatatgt ttactaaagg ac
//
