LOCUS       sequence-82400-e        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the endoplasmic reticulum (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Strack RL, Hein B, Bhattacharyya D, Hell SW, Keenan RJ, Glick BS
  TITLE     A rapidly maturing far-red derivative of DsRed-Express2 for 
            whole-cell labeling.
  JOURNAL   Biochemistry. 2009 Sep 8;48(35):8279-81.
  PUBMED    19658435
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2009-09-8"; volume: "48(35)"; pages: 
            "8279-81"
COMMENT     Sequence Label: pEF.myc.ER-E2-Crimson
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..65)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(75..749)
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(332..351)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     sig_peptide     complement(join(771..782,865..909))
                     /codon_start=1
                     /product="modified signal sequence from the mouse 
                     immunoglobulin heavy chain variable region"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     CDS             complement(join(771..781,864..909))
                     /codon_start=1
                     /product="signal sequence from the mouse immunoglobulin 
                     heavy chain variableregion"
                     /label=IgH signal sequence
                     /translation="MGWSCIILFLVATATGAHS"
     regulatory      906..915
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(955..975)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gactacagct cgtccttctc gcttgcggcc ccattcagat cctcttctga gatgagtttt
       61 tgttctgcgg ccgcctggaa caggtggtgg cgggcctcgg cgcgctcgta ctgctccacc
      121 acggtgtagt cctcgttgtg ggaggtgatg tcgagcttgt agtccacgta gtggtagccg
      181 ggcagcttca cgggcttctt ggccatgtag atggacttga actcacacag gtagtggccg
      241 ccgcccttca gcttcagcgc catgtggttc tcgcccttca gcacgccgtc gcgggggtag
      301 ttgcgctcag tggagggctc ccagcccaga gtcttcttct gcattacggg gccgtcggag
      361 gggaagttca cgccgatgaa cttcacgtgg tagatgaggg tgccgtcctg cagggaggag
      421 tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag
      481 ccctcgggga aggactgctt gaggtagtcg gggatgtcgg cggggtgctt gatgtacgcc
      541 ttggagccgt agaagaactg gggggacagg atgtcccagg cgaagggcag ggggccgccc
      601 ttggtcactt gcagcttggc ggtctgggtg ccctcgtagg gcttgccctc gcccacgccc
      661 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaag
      721 ggcttgatga cgttctcagt gctatccatg tcgacctgca gttggacctg ggagtgcgcg
      781 cctgtggaga gaaaggcaaa gtggatgtca ttgtcaccca tatatatgtc cagacctcaa
      841 gcctgctact gttaacccct tacctgtagc tgttgctacc aagaagagga tgatacagct
      901 ccatcccatg gtggccacgt gttcacgaca cctgaaatgg aagaaaaaaa ctttgaacca
      961 ctgtctgagg cttgagaatg aaccaagatc caaactcaaa aagggcaaat tccaaggaga
//
