LOCUS       sequence-82406-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Miyoshi H, Blomer U, Takahashi M, Gage FH, Verma IM
  TITLE     Development of a self-inactivating lentivirus vector.
  JOURNAL   J Virol. 1998 Oct . 72(10):8150-7.
  PUBMED    9733856
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1998 Oct . 72(10):8150-7."
COMMENT     Sequence Label: pLS-CG
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(77..280)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(106..126)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        281..584
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(908..952)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atggtggcga ccggtagcgc tagcggatct
       61 gacggttcac taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
      121 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
      181 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
      241 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
      301 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
      361 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
      421 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
      481 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
      541 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
      601 ggctatgaac taatgacccc gtaattgatt actattaatc gaattcctgc agcccggggg
      661 atccgttcac taatcgaatg gatctgtctc tgtctctctc tccaccttct tcttctattc
      721 cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat
      781 atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt aaacctacca
      841 agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt atgttaaacc
      901 aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc ttttcttgct
      961 ggttttgcga ttcttcaatt aaggagtgta ttaagc
//
