LOCUS       sequence-82407-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive sfGFP expression plasmid for use in Geobacillus and E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Blanchard K, Robic S, Matsumura I
  TITLE     Transformable facultative thermophile Geobacillus stearothermophilus
            NUB3621 as a host strain for metabolic engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2014 May 2.
  PUBMED    24788326
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol. 2014 May 2."
COMMENT     Sequence Label: pIM1773-PRHIII-sfGFP-pNW33N
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    6..26
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(join(27..740,741..743,744..746))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     misc_feature    874..895
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 catgcctgca gcggccgcta ctagtattac ttgtataatt catccattcc gtgggtgatt
       61 ccggctgcgg tgacaaattc cagcagtacc atgtggtcgc gtttttcatt cggatctttg
      121 gacaggaccg actgtgtaga taagtagtgg ttatccggta gtaatacggg tccatcgccg
      181 atcggtgtat tttgctggta atgatccgct aattggacgc tgccgtcttc cacattatgg
      241 cggattttga agttggcttt gatcccgttt ttttgtttgt ccgcggttat atagacgtta
      301 tgcgagttga agttatactc caacttgtgc cctagtatgt tcccatcttc tttaaagtcg
      361 atgcctttaa gctcaattct gttgacaagc gtgtcgccct caaatttcac ttcggcacgt
      421 gttttatacg ttccatcgtc tttaaaagaa atcgtgcgct cttggacata gccctccggc
      481 atcgccgact tgaaaaagtc gtgccgtttc atgtgatcgg gataccgtga gaaacattgt
      541 actccatagg tcagcgttgt caccagcgtc ggccacggta ctggtaattt ccccgtagta
      601 cagataaatt tgagcgtcaa tttaccgttc gtggcatcgc cttctccttc accgcgcacg
      661 ctgaatttat gtccattaac atcgccgtcc aattctacca aaatcgggac aactcccgta
      721 aacaattcct cgcctttgga gcccatggcc attcactcct agtcaccatt gttttttctt
      781 ttgcatatca tagcagtcgt atcttttcat tatatcagca ttcatggtat gataaacaat
      841 aggattttgc agaatggggg aaatcagcat gctctctaga agcggccgcg aattcaattt
      901 cacaatcaga gagaacggga aagtaaaacg aatcccgata ttgataaaga acggtcacat
      961 gaaaattatg atttg
//
