LOCUS       sequence-82409-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 
            DNA-binding domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Guilinger JP, Thompson DB, Liu DR
  TITLE     Fusion of catalytically inactive Cas9 to FokI nuclease improves the 
            specificity of genome modification.
  JOURNAL   Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909.
  PUBMED    24770324
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909."
COMMENT     Sequence Label: FokI-dCas9
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..644
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 ccaagaagaa gaggaaggtg ggcattcacc gcggggtacc tggaggttct ggatcccaac
       61 tagtcaaaag tgaactggag gagaagaaat ctgaacttcg tcataaattg aaatatgtgc
      121 ctcatgaata tattgaatta attgaaattg ccagaaattc cactcaggat agaattcttg
      181 aaatgaaggt aatggaattt tttatgaaag tttatggata tagaggtaaa catttgggtg
      241 gatcaaggaa accggacgga gcaatttata ctgtcggatc tcctattgat tacggtgtga
      301 tcgtggatac taaagcttat agcggaggtt ataatctgcc aattggccaa gcagatgaaa
      361 tgcaacgata tgtcgaagaa aatcaaacac gaaacaaaca tatcaaccct aatgaatggt
      421 ggaaagtcta tccatcttct gtaacggaat ttaagttttt atttgtgagt ggtcacttta
      481 aaggaaacta caaagctcag cttacacgat taaatcatat cactaattgt aatggagctg
      541 ttcttagtgt agaagagctt ttaattggtg gagaaatgat taaagccggc acattaacct
      601 tagaggaagt cagacggaaa tttaataacg gcgagataaa ctttagcggc agcgagactc
      661 ccgggacctc agagtccgcc acacccgaaa gtgataaaa
//
