LOCUS       sequence-82411-e         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 
            DNA-binding domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Guilinger JP, Thompson DB, Liu DR
  TITLE     Fusion of catalytically inactive Cas9 to FokI nuclease improves the 
            specificity of genome modification.
  JOURNAL   Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909.
  PUBMED    24770324
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909."
COMMENT     Sequence Label: FokI-dCas9
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctatctcct gttcgctttt cgcgatcatc ttacggacgt cataaacttt gtaatcacca
       61 tacacaaact cactttctag cttcgggtat ttcttaatga gtgcggtccc tacgacggca
      121 ttaagataag cgtcgtgcgc atggtggtag ttatttatct ccctaacttt atagaattga
      181 aaatcctttc tgaagtccga caccaatttt gactttaaag tgattacttt gacttcccga
      241 atcagcttat cgttctcgtc gtatttcgta ttcattcggg aatctagtat ctgtgcaaca
      301 tgctttgtga tttggcgggt ttccacgagc tgacgtttaa taaatccggc cttgtcaagt
      361 tcagacaagc cacccctctc agctttagtt aagttatcga actttctttg cgttatcagt
      421 ttcgcattta ggagctgccg ccaatagttc ttcattttct ttacgacttc ctcgcttgga
      481 acattgtcac ttttccctcg gttcttatcc gagcgtgtaa gcactttatt gtcgattgaa
      541 tcgtccttca aaaaggattg gggtacaatg gcatcgacgt cgtaatcaga taaacggttt
      601 atgtccagtt cctgatcaac atacatgtcc cttccatttt gtaggtaata gaggt
//
