LOCUS       sequence-82423-e        6328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ae aegypti hsp70Aa promoter driving firefly luciferase .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Gross TL, Myles KM, Adelman ZN
  TITLE     Identification and characterization of heat shock 70 genes in Aedes 
            aegypti (Diptera: Culicidae).
  JOURNAL   J Med Entomol. 2009 May;46(3):496-504.
  PUBMED    19496419
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Med 
            Entomol."; date: "2009-05"; volume: "46(3)"; pages: "496-504"
COMMENT     Sequence Label: AaHsp70Aa-1373-FFluc
FEATURES             Location/Qualifiers
     source          1..6328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     377..396
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(615..719)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      746..1201
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(833..852)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1043..1064
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    1332..1380
                     /note="synthetic polyadenylation signal"
     misc_feature    1394..1485
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1434..1453
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     CDS             3090..4742
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3156..3174)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    4783..4904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4794..4813)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4848..4867
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5152..5169)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5323..5911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5403..5422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6082..6328,1..545,546..614))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
       61 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
      121 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
      181 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
      241 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
      301 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
      361 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
      421 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
      481 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
      541 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
      601 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
      661 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
      721 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
      781 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      841 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      901 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
      961 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     1021 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     1081 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     1141 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     1201 tgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     1261 ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg ggaggtactt
     1321 ggagcggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
     1381 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa
     1441 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctatcga taggtaccga
     1501 gctctgtaca tgtccgcggt cgcgacgtac gcgtaattat gcagaataag aagaatgttc
     1561 gagagtgttt aagaacttca atcttgaagc tgaaaaacag gggagttata gattgtgcag
     1621 aatggtctgg aactttctag tatagcaatt tttcattggg tataaagatt ttttctccgc
     1681 aaaatatgca ggtgaggagt ttagatgctg tacggctcat ccctgcatga agatagtcca
     1741 gttctaacat tcattttttt tttaattttt tttcgtcaat tttccccaga tcgttatttg
     1801 ggggcatgcg atccaaacaa acaacttcta ctatggttct ctaactcaat atcgagatac
     1861 ggtatatcga gtaacgaaga gttgacttta taatgttttg tgctcctaga gttttagttc
     1921 taatttgaac gatttcgatc aaatgtaagg agttattgag ttatattaga tttttatgaa
     1981 aatgataggt ttctgattga ccaataaaac ctttacacac atttcatact aatcgaatcg
     2041 tttatttttg gacgcagatg tctttgatca cagatggaaa tataaaccaa tgagtcacat
     2101 tgcaataatt ttccgcaggc aaagtgtgtg caggatacat attctcgaaa catgatgcta
     2161 aaagtcgatt aaaaacaaaa catacgcacc cacacatgct agaatgttag cgaatgtcct
     2221 cgaattttct gctgaacgaa cgaaccatag tattagactg gtccaccagt tggatgttgc
     2281 tttaaagaaa agaatgatta ttttcgaatg atgtattccg gatggatttc ttacgcaaca
     2341 aatgtgcatc ctaaccaaaa gtatttaaaa tatttgtaga acaaaaattt tcaaatataa
     2401 aacttatatt atttcgaact atttcacata gaatccactt tctgacaggg gctgggaaaa
     2461 actcgaaatt tcataattaa aaagttgaaa ttttatttag atctgtgaaa acaaatgcaa
     2521 tgttcaaatg tttgaaaggg aacaaaacta aagcatgcca aatttagtat ggaaatcgac
     2581 tgtttttatg ttaaatgttt tttgaactca tgttgtaatt ttttcatcat tgttgtttta
     2641 taattccaat ccctttctag atataaaccg cttgtgtcaa cttcagaaat aatacatttt
     2701 atatttttga accagtctaa aacagcgtcc attgaacgaa cacattaaaa cagtatgaat
     2761 atgacctaac gaaaagcgaa gacaccgtca tcgttcgagc ccatactcat acgaaagtac
     2821 tcgaacaccc gtggaaagaa ctcgaacttt ctagatctgc tcgtttctac tatataagcg
     2881 cccggttcga gcagctctcg tcataacagt tcaaacaaga gaacgaatca caagcaagag
     2941 cgataaagcg ccaaaacgca aatctacgca agtgaaaagc aaatttcaac gtgaaacaaa
     3001 tcgaagcagt gaaataagtg aatacagtga aaaagtgtat catttcgaat caaaaagtgt
     3061 tgaacatcag tacgaaagca aaaatgtcca tggaagacgc caaaaacata aagaaaggcc
     3121 cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga
     3181 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca
     3241 tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg
     3301 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc
     3361 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg
     3421 aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa
     3481 aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta
     3541 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc
     3601 atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga
     3661 caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc
     3721 ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa
     3781 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta
     3841 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag
     3901 agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc
     3961 tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg
     4021 aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt
     4081 tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca gctattctga
     4141 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag
     4201 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt
     4261 gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct
     4321 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
     4381 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc
     4441 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag
     4501 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
     4561 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
     4621 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
     4681 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt
     4741 aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt
     4801 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     4861 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     4921 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     4981 tctacaaatg tggtaaaatc gataaggatc cgtcgaccga tgcccttgag agccttcaac
     5041 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     5101 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac
     5161 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5221 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5281 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5341 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5401 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5461 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5521 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5581 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5641 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5701 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5761 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5821 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5881 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5941 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6001 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6061 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6121 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6181 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6241 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6301 aactttatcc gcctccatcc agtctatt
//
