LOCUS       sequence-82437-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  JxOFF-pre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Kim J, Zhao T, Petralia RS, Yu Y, Peng H, Myers E, Magee JC
  TITLE     mGRASP enables mapping mammalian synaptic connectivity with light 
            microscopy.
  JOURNAL   Nat Methods. 2011 Dec 4;9(1):96-102. doi: 10.1038/nmeth.1784.
  PUBMED    22138823
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-4- 4"; volume: "9"; 
            issue: "1"; pages: "96-102"
COMMENT     Sequence Label: paavCAG-JxOFF-pre-mGRASP-mCerulean
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(86..119)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             227..274
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
       61 catcattttg gcaaagaatt ggatcataac ttcgtatagc atacattata cgaacggtaa
      121 agcttgatga tccatgccac cttctactag tcttttgttg cttgctgcac ttctcccctt
      181 cgctcttccc gcttcagatt ggaagacagg agaagtcacc gcttcccgcg accacatggt
      241 gctgcacgaa tatgtgaacg ctgctggcat cacaggcggt ggcggatctg gcggcggtgg
      301 tagcgtcgac tttcagaaag ctagctctat tgtttataag aaggaggggg aacaggtgga
      361 gttcagtttc ccattggcct tcacagtcga gaagctcaca ggatcaggag agttgtggtg
      421 gcaggccgaa agagcctcct ccagcaagag ttggatcact tttgatctga aaaacaaaga
      481 agtgagcgtg aagagagtca cacaggaccc taagctgcag atgggcaaga agttgccttt
      541 gcacttgaca ctcccccaag ccctgcccca gtatgctggc tccgggaacc tcaccctcgc
      601 cttggaggct aagaccggaa agttgcatca ggaggtcaac ctcgtggtga tgagggcaac
      661 acagctgcag aagaacctga cctgcgaagt gtggggtccc acctcaccta aactgatgct
      721 gtctctgaag ctggagaata aggaggctaa agtcagtaaa agagagaagg ctgtgtg
//
