LOCUS       sequence-82442-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Historical plasmid. For inhibition of neuronal activity, note that 
            Addgene Plasmid #66709 is a light-gated chloride channel with 
            improved anion selectivity (iChloC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Wietek J, Wiegert JS, Adeishvili N, Schneider F, Watanabe H, Tsunoda
            SP, Vogt A, Elstner M, Oertner TG, Hegemann P
  TITLE     Conversion of Channelrhodopsin into a Light-Gated Chloride Channel.
  JOURNAL   Science. 2014 Mar 27.
  PUBMED    24674867
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2014 Mar 27."
COMMENT     Sequence Label: pAAV Syn ChR E90R-D156N-T159C 2A tDimer
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgtcgtgcc tgagagcgca gtcgaattca agctgctagc caccatggat tatggaggcg
       61 ccctgagtgc cgttgggcgc gagctgctat ttgtaacgaa cccagtagtc gtcaatggct
      121 ctgtacttgt gcctgaggac cagtgttact gcgcgggctg gattgagtcg cgtggcacaa
      181 acggtgccca aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac
      241 tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg
      301 tgtgcgctat caggatggtc aaggtgattc tcgagttctt cttcgagttt aagaacccgt
      361 ccatgctgta tctagccaca ggccaccgcg tccagtggtt gcgttacgcc gagtggcttc
      421 tcacctgccc ggtcattctc attcacctgt caaacctgac gggcttgtcc aacgactaca
      481 gcaggcgcac catgggtctg cttgtgtcta atattggctg tattgtgtgg ggcgccactt
      541 ccgccatggc caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg
      601 ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccacacc gtgccgaagg
      661 gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca tggggtatgt
      721 tccccatcct gttcatcctc ggccccgagg gcttcggcgt cctgagcgtg tacggctcca
      781 ccgtcggcca caccatcatt gacctgatgt cgaagaactg ctggggtctg ctcggccact
      841 acctgcgcgt gctgatccac gagcatatcc tcatccacgg cgacattcgc aagaccacca
      901 aattgaacat tggtggcact gaaa
//
