LOCUS       sequence-82445-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Historical plasmid. For inhibition of neuronal activity, note that 
            Addgene Plasmid #66709 is a light-gated chloride channel with 
            improved anion selectivity (iChloC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Wietek J, Wiegert JS, Adeishvili N, Schneider F, Watanabe H, Tsunoda
            SP, Vogt A, Elstner M, Oertner TG, Hegemann P
  TITLE     Conversion of Channelrhodopsin into a Light-Gated Chloride Channel.
  JOURNAL   Science. 2014 Mar 27.
  PUBMED    24674867
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2014 Mar 27."
COMMENT     Sequence Label: pAAV Syn ChR E90R-T159C 2A tDimer
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgtcgtgcct gagagcgcag tcgaattcaa gctgctagcc accatggatt atggaggcgc
       61 cctgagtgcc gttgggcgcg agctgctatt tgtaacgaac ccagtagtcg tcaatggctc
      121 tgtacttgtg cctgaggacc agtgttactg cgcgggctgg attgagtcgc gtggcacaaa
      181 cggtgcccaa acggcgtcga acgtgctgca atggcttgct gctggcttct ccatcctact
      241 gcttatgttt tacgcctacc aaacatggaa gtcaacctgc ggctgggagg agatctatgt
      301 gtgcgctatc aggatggtca aggtgattct cgagttcttc ttcgagttta agaacccgtc
      361 catgctgtat ctagccacag gccaccgcgt ccagtggttg cgttacgccg agtggcttct
      421 cacctgcccg gtcattctca ttcacctgtc aaacctgacg ggcttgtcca acgactacag
      481 caggcgcacc atgggtctgc ttgtgtctga tattggctgt attgtgtggg gcgccacttc
      541 cgccatggcc accggatacg tcaaggtcat cttcttctgc ctgggtctgt gttatggtgc
      601 taacacgttc tttcacgctg ccaaggccta catcgagggt taccacaccg tgccgaaggg
      661 ccggtgtcgc caggtggtga ctggcatggc ttggctcttc ttcgtatcat ggggtatgtt
      721 ccccatcctg ttcatcctcg gccccgaggg cttcggcgtc ctgagcgtgt acggctccac
      781 cgtcggccac accatcattg acctgatgtc gaagaactgc tggggtctgc tcggccacta
      841 cctgcgcgtg ctgatccacg agcatatcct catccacggc gacattcgca agaccaccaa
      901 attgaacatt ggtggcactg
//
