LOCUS       sequence-82446-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sfGFP under the PompC1157 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
  TITLE     Characterizing bacterial gene circuit dynamics with optically 
            programmed gene expression signals.
  JOURNAL   Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
  PUBMED    24608181
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884."
COMMENT     Sequence Label: pEO100c
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(31..88)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    89..109
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(136..849)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 gagcgaggaa gcctgcataa cgcgaagtaa tcttttcggt tttaaagaaa aagggcaggg
       61 tggtgacacc ttgccctttt ttgccggact gcagcggccg ctactagtat ccttacgcga
      121 aatacgggct catcatttgt acagttcatc cataccatgc gtgatgcccg ctgcggttac
      181 gaactccagc agaaccatat gatcgcgttt ctcgttcgga tctttagaca gaacgctttg
      241 cgtgctcaga tagtgattgt ctggcagcag aacaggacca tcaccgattg gagtgttttg
      301 ctggtagtga tcagccagct gcacgctgcc atcctccacg ttgtggcgaa ttttaaaatt
      361 cgctttaatg ccattttttt gtttatcggc ggtgatgtaa acattgtggc tgttaaaatt
      421 gtattccagc ttatggccca ggatattgcc gtcttcttta aagtcaatgc ctttcagctc
      481 aatgcggttt accagggtat cgccttcaaa tttcacttcc gcacgcgttt tgtacgtgcc
      541 gtcatcctta aaggaaatcg tgcgttcctg cacatagcct tccggcatgg cggacttgaa
      601 gaagtcatgc tgcttcatat ggtccggata acgagcaaag cactgaacac cataagtcag
      661 cgtcgttacc agagtcggcc aaggtaccgg cagtttacca gtagtacaga tgaacttcag
      721 cgtcagttta ccattagttg cgtcaccttc accctcgcca cgcacggaaa acttatgacc
      781 gttgacatca ccatccagtt ccaccagaat agggacgaca ccagtgaaca gctcttcgcc
      841 tttacgcata tattgaccta ccttatttta tttatataga cagatttccc ctcggtgct
//
