LOCUS       sequence-82448-e        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cph8 under a modified PLtetO-1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
  TITLE     Characterizing bacterial gene circuit dynamics with optically 
            programmed gene expression signals.
  JOURNAL   Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
  PUBMED    24608181
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884."
COMMENT     Sequence Label: pCph8
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..96)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
       61 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctctagatta cccttctttt
      121 gtcatgccct gcgcccgcgt taccggcact ggcagccagg cgcgaatgga aagcccgccc
      181 cgctcgctgg tgccaagctc cagcatcccg ttatggttat ccacgatacg ctgcacaatt
      241 gccagcccta atcccgtgcc gctaatggtg cgcgcactgt cgccgcggac aaacggctgg
      301 aacaggtgct tacgttgttc cggcgcaatt cccggaccgt catcttccac ctggaaccag
      361 gcgcgattcg gctccgttcc gctgctgact ttgacccagc cattgccata acgggcggcg
      421 ttgaccacca tattcgccac cgcgcgtttg atcgacagcg ggtgcatttt cacttcaatg
      481 ctgccggggt aaagcgcggt ttcaatttcc cgctcatagc cactttcggc agcaatcacc
      541 tcaccgagta ctgcattaag atccgccatt tccatcggca tctcctgccc ggtgcgcagg
      601 tagtcgataa actgctcaat gatggcgttg cactcttcga tatctttatt gatcgattct
      661 gccagatagc catcctgctc gctcatcatc tcagtcgcca ggcgaatacg cgtcagcggc
      721 gtgcgcaagt cgtgacttac ccccgccatc agcagcgtgc ggtcatccgc cagttgctta
      781 acaccagccg ccatatgcaa ttcttctgcc tggcgcaaaa tgaggttgac gatcgccttt
      841 ttcagggcca gggcactttg gatttccacc gattgccagg gcaaagattg gagtcggaca
      901 atttctttcc agaggtcaaa ggattggcgg ggatggagct cgattttacc gtcttcctgg
      961 gtagcttcgt aagcatgatt tgggtcaccg ccccaattaa ccgtttgcaa cacttcaggg
     1021 cgaaaccaga gcaaaaagtt gtgacgggca atgggaatgg ccaataagcc actggccacg
     1081 gattta
//
