LOCUS       sequence-82449-e        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
  TITLE     Characterizing bacterial gene circuit dynamics with optically 
            programmed gene expression signals.
  JOURNAL   Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
  PUBMED    24608181
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884."
COMMENT     Sequence Label: pJT119b
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgccggaagg ctatgtgcag gaacgcacga tttcctttaa ggatgacggc acgtacaaaa
       61 cgcgtgcgga agtgaaattt gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca
      121 ttgactttaa agaagacggc aatatcctgg gccataagct ggaatacaat tttaacagcc
      181 acaatgttta catcaccgcc gataaacaaa aaaatggcat taaagcgaat tttaaaattc
      241 gccacaacgt ggaggatggc agcgtgcagc tggctgatca ctaccagcaa aacactccaa
      301 tcggtgatgg tcctgttctg ctgccagaca atcactatct gagcacgcaa agcgttctgt
      361 ctaaagatcc gaacgagaaa cgcgatcata tggttctgct ggagttcgta accgcagcgg
      421 gcatcacgca tggtatggat gaactgtaca aatgatgatt tttttgacgg taaagccaag
      481 ctgaaggctg acaaaatcta tactgttata tccccctttc ctgggggact taggcggttt
      541 aataactggg gtttttgcta gaaatatttt ggcgtttaat tatttattct ctggtttaat
      601 atttcgagac tttgcagact ttgtatttga ggccagactc caatgttgaa actagccggt
      661 aaaaaagcct ctgccctgac ctgcaagaaa acgaaggcaa aaattggtat cctagaaatg
      721 gatcggcagt ggtatcaatc agtcaagcga tttttatgtc caagttttga aatttctgct
      781 atcaacttcc aagatatttt gtttaacaat gttgacgttg gagaatatca ttcgatccta
      841 gttggttgcg ggattaaata tgaatcaatt gatatttcag ctactttaga tttagtgacc
      901 attattaaaa aattgtctgc taagccgccg gccctactac taattacgga ttgtgccgac
      961 agtcagatca cggtacaagc tcgcagttac ctgccccaaa ttgacggcgt ttttgccaag
     1021 gatcatgatt tagctttatt gttaaaagtc atgaaaatta ttgccaaaca aaaatatttt
//
